CROSS-PALMGRENS METHOD

SECTIONS

8 micron paraffin wax sections

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SOLUTIONS

1. Reducer

• Pyrogallol 10g
  
• Distilled water 450ml
  
• Absolute alcohol 550ml
  
• 1% Nitric acid 2ml
  

2. 1% ammonia

3. Silver Solution

• Silver nitrate 15g
  
• Potassium nitrate 10g
  
• Distilled water 100ml
  
• 5% Acetic acid 1ml
  

4. 0.2% Gold Chloride

5. 5% Sodium Thiosulphate

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METHOD

1. Place reducer in 60C incubator for use at stage 5
2. Take sections to water
3. Place in 1% ammonia for 5 minutes
4. Rinse in distilled water
5. Impregnate with silver solution for 30-45 minutes
6. Drain excess silver solution from the slides and without rinsing, pour on the warm reducer until the sections turn brown. Slight agitation prevents deposit formation
7. Wash in distilled water
   
8. Tone in 0.2% gold chloride for 2 minutes
   
9. Wash in water
   
10. Fix in 5% sodium thiosulphate solution
    
11. Wash in water
    
12. Dehydrate, clear . Mount sections in DPX
    

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RESULTS

• Neurofibrillary Tangles - Black
• Senile plaque neurites - Black
• Background - Grey

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

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© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk