GRIMELIUS

1. Sections to water.

2. Treat sections at 65ªc with silver solution for 3 hours

3. Drain silver solution from slide.

4. Treat with freshly prepared reducing solution at 45ªc for 1 minute.

5. Wash in distilled water

6. Repeat step 4 until the desired result is achieved.

7. Wash in distilled water.

8. Dehydrate, clear . Mount sections in DPX

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RESULTS

--Argyrophil material black

--Background yellow

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SOLUTIONS

SILVER STAINING SOLUTION

-Acetate buffer ph 5.6 10 mls

--Distilled water 87 mls

--1% silver nitrate 3 mls

REDUCING SOLUTION

--Sodium sulphite (hydrated) 2.5 g

--Hydroquinone (quinol) 0.5 g

--Distilled water 50 mls

ACETATE BUFFER pH 5.6

--0.2M acetic acid 4.8 mls

--0.2M sodium acetate 45.2 mls

--Distilled water 50 mls

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

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© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk