HAEMATOXYLIN AND EOSIN TECHNIQUE

SOLUTIONS

(i) Gills Haematoxylin

• Haematoxylin 6.0g
  
• Alluminium Sulphate 4.2g
  
• Citric Acid 1.4g
  
• Sodium Iodate 0.6g
  
• Ethylene Glycol 269ml
  
• Distilled Water 680ml
  

(ii) Eosin

• Eosin Yellowish 1.0g
  
• Distilled Water 100ml
  

(iii) Lithium Carbonate 1%

• Lithium Carbonate 1g
  
• Distilled Water 100g
  

(iv) Acid Alcohol 1%

• 70% Alcohol 99ml
  
• conc. Hydrochloric Acid 1ml
  

(v)Scott's tap water

• In a beaker containing 1L distilled water, add 20g sodium bicarbonate and 3.5g magnesium sulphate. add a magnetic stirrer and mix thoroughly to dissolve the salts. Using a filter funnel, pour the solution into a labelled bottle
  

METHOD

1. Take sections to water.
   
2. Place sections in haematoxylin for 5 minutes.
   
3. Wash in tap water.
   
4. 'Blue' sections in lithium carbonate or Scott's tap water.
   
5. Wash in tap water.
   
6. Place sections in 1% acid alcohol for a few seconds.
   
7. Wash in tap water.
   
8. Place sections in eosin for 5 minutes.
   
9. Wash in tap water.
   
10. Dehydrate, clear . Mount sections in DPX
    

RESULTS

• Nuclei Blue-black
  
• Cytoplasm Varying shades of pink
  
• Muscle fibres Deep pinky red.
  
• Fibrin Deep pink
  
• Red blood cells Orange/red
  

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

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© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk