MELANIN BLEACH

SECTIONS
4µm paraffin wax sections

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SOLUTIONS
a) 0.5% acidified potassium permanganate
b) 1% oxalic acid

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METHOD

1. Take sections to water
   
2. Treat sections with 0.5% acidified potassium permanganate for 30 minutes
   
3. Wash in water
   
4. Treat sections with 1% oxalic acid for 5-10 minutes
   
5. Wash, examine and re-bleach if necessary
   
6. When bleached, counterstain as desired eg H&E
   
7. Dehydrate, clear and mount in DPX
   

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

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© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk