METHYL GREEN PYRONIN

1. Bring sections to water.

2. Rinse with Acetate Buffer pH 4.8.

3. Stain with the MGP solution 30 mins.

4. Wash with buffer and blot dry.

5. Rinse in equal parts acetone/xylene, then xylene.

6. Mount sections in DPX

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RESULTS

--DNA (Nuclei) Green or Green/Blue

--RNA (Plasma cell cytoplasm, nissl substance, bacteria) Red

--Some mucins Red

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SOLUTIONS

--2% Aqueous methyl green (extract with chloroform until methyl violet contaminant is removed) 9 ml

--2% Aqueous pyronin Y or G 4ml

--Glycerol 14ml

--pH 4.8 buffer 23ml

--MIX BEFORE USE

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

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© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk