PERLS TECHNIQUE

SECTIONS

4µm paraffin wax sections

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SOLUTIONS

1. INCUBATING SOLUTION

--2% Potassium ferrocyanide 10mls

--2% Hydrochloric acid 10mls

--Prepare fresh before use

2. 0.1% Nuclear fast red

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METHOD

1. Take sections to water

2. Place in freshly prepared incubating solution for 10 minutes

3. Wash well in water

4. Counterstain in 0.1% nuclear fast red for 5 minutes

5. Wash in water

6. Dehydrate, clear . Mount sections in DPX

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RESULTS

--Ferric iron - Blue

--Nuclei - Red

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

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© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk