RESIN STAINING METHODS

HAEMATOXYLIN AND EOSIN

1. Stain in Gill's double strength haematoxylin 10-20 minutes

2. Wash in distilled water.

3. Blue in Scotts tap water substitute.

4. Wash in water for 15 minutes.

5. Stain in 1% eosin in filtered 1% calcium chloride for 2-4 minutes.

6. Wash in water for 30 seconds.

7. Blot dry.

8. Wash in 100% alcohol for 20 seconds.

9. Rinse in xylene.

10. Mount in DPX.

RESULTS

--Nuclei Blue

--Cytoplasm Pink

--Muscle Red

--Collagen Pink

--RBCs Orange/red

---

GILLS DOUBLE STRENGTH HAEMATOXYLIN

--Haematoxylin 4g

--Distilled water 730ml

--Ethylene glycol 250ml

--Aluminium sulphate (Hydrated) 35g

--Acetic acid 20ml

Then add sodium iodate 0.4g

SCOTT'S TAP WATER SUBSTITUTE

--Potassium bicarbonate 1g

--Magnesium sulphate 10g

--Distilled water 500ml

---

PERIODIC ACID SCHIFF (PAS)

1. Treat solutions with 1% periodic acid for 20 minutes.

2. Rinse in distilled water.

3. Treat sections with Schiff's reagent for 30 minutes.

4. Wash in distilled water for 20 minutes.

5. Counterstain in Gill's haematoxylin for 20 seconds.

6. Blue.

7. Wash in water.

8. DCM.

RESULTS

-PAS positive material Magenta

--Nuclei Blue

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

---

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk