RUBEANIC ACID TECHNIQUE

1. Sections to water.

2. Place in staining solution overnight at 37ªc.

3. Wash in water.

4. Counterstain in neutral red or nuclear fast red.

5. Rinse in absolute alcohol.

6. Clear in xylene. Mount sections in DPX

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RESULTS Copper green/black Granules

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CONTROLS

Essential

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SOLUTIONS

RUBEANIC ACID STOCK

--Rubeanic acid 50 mg

--Absolute alcohol 50 mls

STAINING SOLUTION

--Rubeanic acid stock 2.5 mls

--10% sodium acetate 50 mls

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

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© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk