TOLUIDINE BLUE

1. Sections to water.

2. Stain in toluidine blue for 10 seconds.

3. Wash in water.

4. Differentiate in 0.2% uranyl nitrate until the background appears colourless. 5. Dehydrate rapidly. Clear . Mount sections in DPX

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RESULTS

Mast cells and some mucins purple/red

Nuclei pale blue

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SAFETY

Uranyl nitrate is radioactive. Use P.P.E.

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

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© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk