Contact

• workRoom C213 Biodiscovery Institute
  University Park
  Nottingham
  NG7 2RD
  UK
• workx87556
• amanda.tatler@nottingham.ac.uk

Biography

Deputy Director of the Biodiscovery Institute

I am a Principal Research Fellow (Associate Prof.) within the Centre for Respiratory Research/ Nottingham BRC in Translational Medical Sciences. My research is focused upon understanding the underlying mechanisms driving tissue remodelling in chronic lung diseases (including idiopathic pulmonary fibrosis, asthma, COPD) and viral infections (such as influenza, SARS-CoV-2). My expertise span in vitro, ex vivo and in vivo models of respiratory disease.

During my post-doctoral years I spent two periods of research working in the USA at UCSF and Harvard Medical School, which were funded by a Royal Society Travel Fellowship (UCSF) and an NC3Rs David Sainsbury Fellowship (Harvard). During these periods of research I perfected techniques not currently employed within Nottingham including isolation of Type II alveolar epithelial cells from human lung, and the use of precision cut lung slices. Following the completion of the NC3Rs Fellowship in 2015 I was awarded a subsequent mid-career fellowship funded jointly by Asthma UK and the Medical Resaerch Foundation.

Ongoing work within my lab group is focused upon:

- understanding the role that matrix cross linking and stiffness plays in promoting tissue remodelling in chronic lung diseases

- investigating how biomechanical cues from breathing may influence pro-remodelling pathways to promote disease

- identifying potential pathways to be exploited to enhance alveolar repair in the lung

I have been actively involved in the British Association for Lung Research (www.balr.co.uk) since 2010: from 2017-2021 I served as Secretary and am currently the BALR Treasurer. I am also a member of the Early Career Working Group of the Respiratory Cell and Molecular Biology Assembly of the American Thoracic Society and sit on the Scientific Committee of the International Colloquium for Lung and Airways Fibrosis.

I am an Associate Editor for Pharmacology and Therapeutics and a Review Editor for Frontiers in Allergy.

Expertise Summary

In vivo and ex vivo models of respiratory diseases including development of novel models to impact the 3Rs;

Molecular biology including chromatin immunoprecipitation and investigating regulation of gene expression;

Functional assays to assess activation of TGF<beta> in vitro and in vivo;

Isolation, culture and characterisation of a variety of primary cells from human and animal tissue;

Flow cytometry;

Immunohistochemistry, immunocytochemistry, immunofluorescence and histological staining

Western blotting and electrophoresis

DNA cloning and mutagenesis

Transfections including plasmids and siRNA constructs

Research Summary

* Developing a novel "breathing" lung slice model by imposing cyclic stretch on precision cut lung slices

* Investigating the role of bronchoconstriction on TGFbeta activation and the developement of asthmatic airway remodelling

* Delineating the role of Elk1 as a master regulator of fibrogenesis

Recent Publications

• JOPETH RAMIS, ROBERT MIDDLEWICK, FRANCESCO PAPPALARDO, JENNIFER T. CAIRNS, IAIN D. STEWART, ALISON E. JOHN, SHAMS-UN-NISA NAVEED, RAMASWAMY KRISHNAN, SUZANNE MILLER, DOMINICK E. SHAW, CHRISTOPHER E. BRIGHTLING, LEE BUTTERY, FELICITY ROSE, GISLI JENKINS, SIMON R. JOHNSON and AMANDA L. TATLER, 2022. Lysyl oxidase-like 2 is increased in asthma and contributes to asthmatic airway remodelling ERJ.
• JOSEPH, CHITRA and TATLER, AMANDA L., 2022. Pathobiology of Airway Remodeling in Asthma: The Emerging Role of Integrins JOURNAL OF ASTHMA AND ALLERGY. 15, 595-610
• RAMIS, JOPETH, MIDDLEWICK, ROBERT, PAPPALARDO, FRANCESCO, CAIRNS, JENNIFER T, STEWART, IAIN D, JOHN, ALISON E, NAVEED, SHAMS-UN-NISA, KRISHNAN, RAMASWAMY, MILLER, SUZANNE, SHAW, DOMINICK E, BRIGHTLING, CHRISTOPHER E, BUTTERY, LEE, ROSE, FELICITY, JENKINS, GISLI, JOHNSON, SIMON R and TATLER, AMANDA L, 2022. Lysyl oxidase like 2 is increased in asthma and contributes to asthmatic airway remodelling. The European respiratory journal. 60(1),
• JOHN, ALISON E., JOSEPH, CHITRA, JENKINS, GISLI and TATLER, AMANDA L., 2021. COVID-19 and pulmonary fibrosis: A potential role for lung epithelial cells and fibroblasts IMMUNOLOGICAL REVIEWS.

• View all publications

Joan Bending, Evelyn Bending, Mervyn Stephens and Olive Stephens Memorial Fellowship - Oct 2015 - Mar 2019 (£300k)

NC3Rs David Sainsbury Fellowship - Oct 2012 to Sept 2015 (£195k)

Royal Society Travel Fellowship - Jan 2011 to Apr 2011 (£4k)

MRF Project Grant (PI) - Oct 2016 to Sept 2019 (£240k)

MRC Project Grant (Co-I) - Oct 2014 to Sept 2018 (£640k)

• JOPETH RAMIS, ROBERT MIDDLEWICK, FRANCESCO PAPPALARDO, JENNIFER T. CAIRNS, IAIN D. STEWART, ALISON E. JOHN, SHAMS-UN-NISA NAVEED, RAMASWAMY KRISHNAN, SUZANNE MILLER, DOMINICK E. SHAW, CHRISTOPHER E. BRIGHTLING, LEE BUTTERY, FELICITY ROSE, GISLI JENKINS, SIMON R. JOHNSON and AMANDA L. TATLER, 2022. Lysyl oxidase-like 2 is increased in asthma and contributes to asthmatic airway remodelling ERJ.
• JOSEPH, CHITRA and TATLER, AMANDA L., 2022. Pathobiology of Airway Remodeling in Asthma: The Emerging Role of Integrins JOURNAL OF ASTHMA AND ALLERGY. 15, 595-610
• RAMIS, JOPETH, MIDDLEWICK, ROBERT, PAPPALARDO, FRANCESCO, CAIRNS, JENNIFER T, STEWART, IAIN D, JOHN, ALISON E, NAVEED, SHAMS-UN-NISA, KRISHNAN, RAMASWAMY, MILLER, SUZANNE, SHAW, DOMINICK E, BRIGHTLING, CHRISTOPHER E, BUTTERY, LEE, ROSE, FELICITY, JENKINS, GISLI, JOHNSON, SIMON R and TATLER, AMANDA L, 2022. Lysyl oxidase like 2 is increased in asthma and contributes to asthmatic airway remodelling. The European respiratory journal. 60(1),
• JOHN, ALISON E., JOSEPH, CHITRA, JENKINS, GISLI and TATLER, AMANDA L., 2021. COVID-19 and pulmonary fibrosis: A potential role for lung epithelial cells and fibroblasts IMMUNOLOGICAL REVIEWS.
• ALGHAMDI, ABDULRHMAN, ALQARNI, ABDULLAH, ALAHMARI, MUSHABBAB, ALQARNI, ABDULLAH, TATLER, AMANDA and PANG, LINHUA, 2021. Effect of cigarette smoke extract on Th17 inflammation and the anti-inflammatory effect of glucocorticoids in human airway smooth muscle cells EUROPEAN RESPIRATORY JOURNAL. 58,
• ALAHMARI, MUSHABBAB, ALGHAMDI, ABDULRHMAN, ALQARNI, ABDULLAH, TATLER, AMANDA and PANG, LINHUA, 2021. Effect of cigarette smoke extract on inflammatory cytokine and chemokine production in human airway smooth muscle cells EUROPEAN RESPIRATORY JOURNAL. 58,
• ALGHAMDI, ABDULRHMAN, ALQARNI, ABDULLAH, ALAHMARI, MUSHABBAB, EDWARDS-PRITCHARD, ROCHELLE, TATLER, AMANDA and PANG, LINHUA, 2021. Effect of cigarette smoke extract and Th17 cytokine on production of inflammatory cytokines and chemokines in human airway smooth muscle cells EUROPEAN RESPIRATORY JOURNAL. 58,
• ALAHMARI, MUSHABBAB, ALGHAMDI, ABDULRHMAN, ALQARNI, ABDULLAH, TATLER, AMANDA and PANG, LINHUA, 2021. Effect of cigarette smoke extract on IL-8 production and anti-inflammatory effect of corticosteroids in human airway smooth muscle cells EUROPEAN RESPIRATORY JOURNAL. 58,
• CAIRNS, J. T., MIDDLEWICK, R. and TATLER, A. L., 2021. CYCLICAL MECHANICAL STRETCHING OF PRECISION CUT LUNG SLICES TO MIMIC BREATHING RESULTS IN ACTIVATION OF TGFB THORAX. 76, A49-A50
• CALVER, J., JOSEPH, C., JOHN, A. E., ORGAN, L., FAINBERG, H., PORTE, J., MUKHOPADHYAY, S., BARTON, L., STROBERG, E., DUVAL, E., COPIN, M., POISSY, J., STEINESTREL, K., TATLER, A. L. and JENKINS, G., 2021. THE NOVEL CORONAVIRUS SARS-COV-2 BINDS RGD INTEGRINS AND UPREGULATES AVB3 INTEGRINS IN COVID-19 INFECTED LUNGS THORAX. 76, A22-A23
• CALTHORPE, REBECCA, SMYTH, ALAN and TATLER, AMANDA, 2021. The role of transforming growth factor beta isoforms in the development of bronchopulmonary dysplasia PEDIATRIC RESEARCH. 90(SUPPL 1), 40-40
• PORTE, JOANNE, JENKINS, GISLI and TATLER, AMANDA L., 2021. Myofibroblast TGF-beta Activation Measurement In Vitro MYOFIBROBLASTS. 2299, 99-108
• PYBUS, HANNAH J., TATLER, AMANDA L., EDGAR, LOWELL T., O'DEA, REUBEN D. and BROOK, BINDI S., 2021. Reduced biomechanical models for precision-cut lung-slice stretching experiments JOURNAL OF MATHEMATICAL BIOLOGY. 82(5),
• CAIRNS, JENNIFER T., HABGOOD, ANTHONY, EDWARDS-PRITCHARD, ROCHELLE C., JOSEPH, CHITRA, JOHN, ALISON E., WILKINSON, CHLOE, STEWART, IAIN D., LESLIE, JACK, BLAXALL, BURNS C., SUSZTAK, KATALIN, ALBERTI, SIEGFRIED, NORDHEIM, ALFRED, OAKLEY, FIONA, JENKINS, GISLI and TATLER, AMANDA L., 2020. Loss of ELK1 has differential effects on age-dependent organ fibrosis INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY. 120,
• TATLER, AMANDA L., 2019. Recent advances in the non-invasive assessment of fibrosis using biomarkers CURRENT OPINION IN PHARMACOLOGY. 49, 110-115
• TATLER, AMANDA L., 2019. MAP(kinase)-ing a Link between Obesity and Inflammation in Severe Asthma AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY. 60(5), 497-498
• HILL, MICHAEL R, PHILP, CHRISTOPHER J, BILLINGTON, CHARLOTTE K, TATLER, AMANDA L, JOHNSON, SIMON R, O'DEA, REUBEN D and BROOK, BINDI S, 2018. A theoretical model of inflammation- and mechanotransduction-driven asthmatic airway remodelling. Biomechanics and modeling in mechanobiology. 17(5), 1451-1470
• HILL, MICHAEL R., PHILP, CHRISTOPHER J., BILLINGTON, CHARLOTTE K., TATLER, AMANDA L., JOHNSON, SIMON R., O'DEA, REUBEN D. and BROOK, BINDI S., 2018. A theoretical model of inflammation- and mechanotransduction-driven asthmatic airway remodelling BIOMECHANICS AND MODELING IN MECHANOBIOLOGY. 17(5), 1451-1470
• JENKINS RG, MOORE BB, CHAMBERS RC, EICKELBERG O, KÖNIGSHOFF M, KOLB M, LAURENT GJ, NANTHAKUMAR CB, OLMAN MA, PARDO A, SELMAN M, SHEPPARD D, SIME PJ, TAGER AM, TATLER AL, THANNICKAL VJ, WHITE ES and , 2017. An Official American Thoracic Society Workshop Report: Use of Animal Models for the Preclinical Assessment of Potential Therapies for Pulmonary Fibrosis. American journal of respiratory cell and molecular biology. 56(5), 667-679
• JOHN AE, PORTE J, JENKINS G and TATLER AL, 2017. Methods for the Assessment of Active Transforming Growth Factor-β in Cells and Tissues. Methods in molecular biology (Clifton, N.J.). 1627, 351-365
• TATLER, AMANDA L., HABGOOD, ANTHONY, PORTE, JOANNE, JOHN, ALISON E., STAVROU, ANASTASIOS, HODGE, EMILY, KERAMA-LIKOKO, CHERYL, VIOLETTE, SHELIA M., WEINREB, PAUL H., KNOX, ALAN J., LAURENT, GEOFFREY, PARFREY, HELEN, WOLTERS, PAUL JOHN, WALLACE, WILLIAM, ALBERTI, SIEGFRIED, NORDHEIM, ALFRED and JENKINS, GISLI, 2016. Reduced Ets Domain-containing Protein Elk1 Promotes Pulmonary Fibrosis via Increased Integrin alpha v beta 6 Expression JOURNAL OF BIOLOGICAL CHEMISTRY. 291(18), 9540-9553
• HABGOOD AN, TATLER AL, PORTE J, WAHL SM, LAURENT GJ, JOHN AE, JOHNSON SR and JENKINS G, 2016. Secretory leukocyte protease inhibitor gene deletion alters bleomycin-induced lung injury, but not development of pulmonary fibrosis. Laboratory investigation; a journal of technical methods and pathology. 96(6), 623-31
• TATLER AL, BARNES J, HABGOOD A, GOODWIN A, MCANULTY RJ and JENKINS G, 2016. Caffeine inhibits TGFβ activation in epithelial cells, interrupts fibroblast responses to TGFβ, and reduces established fibrosis in ex vivo precision-cut lung slices. Thorax. 71(6), 565-7
• TATLER AL, GOODWIN AT, GBOLAHAN O, SAINI G, PORTE J, JOHN AE, CLIFFORD RL, VIOLETTE SM, WEINREB PH, PARFREY H, WOLTERS PJ, GAULDIE J, KOLB M and JENKINS G, 2016. Amplification of TGFβ Induced ITGB6 Gene Transcription May Promote Pulmonary Fibrosis. PloS one. 11(8), e0158047
• JOHN AE, WILSON MR, HABGOOD A, PORTE J, TATLER AL, STAVROU A, MIELE G, JOLLY L, KNOX AJ, TAKATA M, OFFERMANNS S and JENKINS RG, 2016. Loss of epithelial Gq and G11 signaling inhibits TGFβ production but promotes IL-33-mediated macrophage polarization and emphysema. Science signaling. 9(451), ra104
• CLIFFORD RL, PATEL JK, JOHN AE, TATLER AL, MAZENGARB L, BRIGHTLING CE and KNOX AJ, 2015. CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET. American journal of physiology. Lung cellular and molecular physiology. 308(9), L962-72
• TATLER AL and JENKINS G, 2015. Reducing affinity of αvβ8 interactions with latent TGFβ: dialling down fibrosis. Annals of translational medicine. 3(Suppl 1), S31
• DAVID M LILBURN, AMANDA L TATLER, JOSEPH S SIX, CLEMENTINE LESBATS, ANTHONY HABGOOD, JOANNE PORTE, THEODORE HUGHES-RILEY, DOMINICK E SHAW, GISLI JENKINS and THOMAS MEERSMAN, 2015. Investigating Lung Responses with Functional Hyperpolarized Xenon-129 in an Ex Vivo Rat Model of Asthma Magnetic Resonance in Medicine. (In Press.)
• AMANDA L TATLER, 2015. Sphingosine-1-Phosphate Metabolism: Can its Enigmatic Lyase Promote the Autophagy of Fibrosis Thorax. (In Press.)
• NOBLE PB, PASCOE CD, LAN B, ITO S, KISTEMAKER LEM, TATLER AL, PERA T, BROOK BS, GOSENS R and WEST AR, 2014. Airway smooth muscle in asthma: Linking contraction and mechanotransduction to disease pathogenesis and remodelling. Pulmonary pharmacology & therapeutics. 29(2), 96-107
• JOLLY L, STAVROU A, VANDERSTOKEN G, MELIOPOULOS VA, HABGOOD A, TATLER AL, PORTE J, KNOX A, WEINREB P, VIOLETTE S, HUSSELL T, KOLB M, STAMPFLI MR, SCHULTZ-CHERRY S and JENKINS G, 2014. Influenza promotes collagen deposition via αvβ6 integrin-mediated transforming growth factor β activation. The Journal of biological chemistry. 289(51), 35246-63
• JOHN AE, LUCKETT JC, TATLER AL, AWAIS RO, DESAI A, HABGOOD A, LUDBROOK S, BLANCHARD AD, PERKINS AC, JENKINS RG and MARSHALL JF, 2013. Preclinical SPECT/CT imaging of αvβ6 integrins for molecular stratification of idiopathic pulmonary fibrosis. Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 54(12), 2146-52
• BEDKE N, SAMMUT D, GREEN B, KEHAGIA V, DENNISON P, JENKINS G, TATLER A, HOWARTH PH, HOLGATE ST and DAVIES DE, 2012. Transforming growth factor-beta promotes rhinovirus replication in bronchial epithelial cells by suppressing the innate immune response. PloS one. 7(9), e44580
• TATLER, A.L. and JENKINS, G., 2012. TGF-β activation and lung fibrosis Proceedings of the American Thoracic Society. 9(3), 130-136
• TATLER, A.L., JOHN, A.E., JOLLY, L., HABGOOD, A., PORTE, J., BRIGHTLING, C., KNOX, A.J., PANG, L., SHEPPARD, D., HUANG, X. and JENKINS, G., 2011. Integrin avβ5-mediated TGF-β activation by airway smooth muscle cells in asthma Journal of Immunology. 187(11), 6094-6107
• MULLAN, C.S., RILEY, M., CLARKE, D., TATLER, A., SUTCLIFFE, A., KNOX, A.J. and PANG, L., 2008. β-tryptase regulates IL-8 expression in airway smooth muscle cells by a PAR-2-independent mechanism American Journal of Respiratory Cell and Molecular Biology. 38(5), 600-608
• TATLER, A.L., PORTE, J., KNOX, A., JENKINS, G. and PANG, L., 2008. Tryptase activates TGFβ in human airway smooth muscle cells via direct proteolysis Biochemical and Biophysical Research Communications. 370(2), 239-242