1. Sections to water.
2. Stain in toluidine blue for 10 seconds.
3. Wash in water.
4. Differentiate in 0.2% uranyl nitrate until the background appears colourless.
5. Dehydrate rapidly. Clear . Mount sections in DPX
Mast cells and some mucins purple/red
Nuclei pale blue
Uranyl nitrate is radioactive. Use P.P.E.
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otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.
© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997
with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk