Cynthia Bosquillon

Contact

• workRoom B05 Boots Science Building
  University Park
  Nottingham
  NG7 2RD
  UK
• work0115 8466078
• cynthia.bosquillon@nottingham.ac.uk

Biography

I graduated as a pharmacist from the Université catholique de Louvain, Belgium in 1998 and then pursued post-graduate studies under the co-supervision of Profs. Rita Vanbever and Véronique Préat. I gained my PhD in September 2003 and in 2004, I moved to London to work on a post-doctoral project directed by Dr.Ben Forbes (King's College London) and Dr.Nicola Clear (Pfizer R&D). In 2005, I was awarded a University of London Maplethorpe fellowship, which gave me, in addition to a valuable teaching experience, the opportunity to develop my own research area and supervise both undergraduate and postgraduate research students at King's. I was appointed to a lectureship in Drug Delivery at Nottingham in 2007.

My research is mainly focused on drug inhalation, of which I've explored different aspects, from the formulation and characterisation of dry powders for systemic delivery of therapeutic proteins to the development and validation of in vitro and ex vivo models of the lungs as tools to predict pulmonary drug absorption in vivo.

Expertise Summary

pulmonary drug delivery; biopharmaceutics; in vitro models; drug transport mechanisms; drug transporters; pro-drugs

Research Summary

My current research interests include:

• the use of respiratory epithelial cell culture models to investigate drug disposition in the lungs, including the impact of drug transporters and lung pathological conditions.
• the development of in vitro systems to study the interactions between drugs/drug particles and airway mucus.
• the development of strategies based on drug design or formulation to optimise drug retention in the lungs.

Recent Publications

• MAHRI, SOHAIB, RONDON, AURELIE, WILMS, TOBIAS, BOSQUILLON, CYNTHIA and VANBEVER, RITA, 2020. Biodistribution and elimination pathways of PEGylated recombinant human deoxyribonuclease I after pulmonary delivery in mice. Journal of controlled release : official journal of the Controlled Release Society.
• M MUKHERJEE, E CINGOLANI, D I PRITCHARD and C BOSQUILLON, 2017. Enhanced expression of Organic Cation Transporters in bronchial epithelial cell layers following insults associated with asthma – Impact on salbutamol transport European Journal of Pharmaceutical Sciences. 106, 62-70
• PANDUGA V, STOCKS MJ and BOSQUILLON C, 2017. Ipratropium is 'luminally recycled' by an inter-play between apical uptake and efflux transporters in Calu-3 bronchial epithelial cell layers. International journal of pharmaceutics. (In Press.)
• BOSQUILLON C, MADLOVA M, PATEL N, CLEAR N and FORBES B, 2017. A Comparison of Drug Transport in Pulmonary Absorption Models: Isolated Perfused rat Lungs, Respiratory Epithelial Cell Lines and Primary Cell Culture. Pharmaceutical Research. 1-9

• View all publications

• MAHRI, SOHAIB, RONDON, AURELIE, WILMS, TOBIAS, BOSQUILLON, CYNTHIA and VANBEVER, RITA, 2020. Biodistribution and elimination pathways of PEGylated recombinant human deoxyribonuclease I after pulmonary delivery in mice. Journal of controlled release : official journal of the Controlled Release Society.
• M MUKHERJEE, E CINGOLANI, D I PRITCHARD and C BOSQUILLON, 2017. Enhanced expression of Organic Cation Transporters in bronchial epithelial cell layers following insults associated with asthma – Impact on salbutamol transport European Journal of Pharmaceutical Sciences. 106, 62-70
• PANDUGA V, STOCKS MJ and BOSQUILLON C, 2017. Ipratropium is 'luminally recycled' by an inter-play between apical uptake and efflux transporters in Calu-3 bronchial epithelial cell layers. International journal of pharmaceutics. (In Press.)
• BOSQUILLON C, MADLOVA M, PATEL N, CLEAR N and FORBES B, 2017. A Comparison of Drug Transport in Pulmonary Absorption Models: Isolated Perfused rat Lungs, Respiratory Epithelial Cell Lines and Primary Cell Culture. Pharmaceutical Research. 1-9
• LUO T, MAGNUSSON J, PRÉAT V, FRÉDÉRICK R, ALEXANDER C, BOSQUILLON C and VANBEVER R, 2016. Synthesis and In Vitro Evaluation of Polyethylene Glycol-Paclitaxel Conjugates for Lung Cancer Therapy. Pharmaceutical research. 33(7), 1671-81
• LUO T, LOIRA-PASTORIZA C, PATIL HP, UCAKAR B, MUCCIOLI GG, BOSQUILLON C and VANBEVER R, 2016. PEGylation of paclitaxel largely improves its safety and anti-tumor efficacy following pulmonary delivery in a mouse model of lung carcinoma. Journal of controlled release : official journal of the Controlled Release Society. 239, 62-71
• 2016. Drug transporters in the lung: Expression and potential impact on pulmonary drug disposition RSC Drug Discovery Series. 2016-January(54), 184-227
• HUTTER V, CHAU DY, HILGENDORF C, BROWN A, COOPER A, ZANN V, PRITCHARD DI, BOSQUILLON C., CHAU DY,, HILGENDORF C,, BROWN A,, COOPER A,, ZANN V,, PRITCHARD DI, and BOSQUILLON C., 2014. Digoxin net secretory transport in bronchial epithelial cell layers is not exclusively mediated by P-glycoprotein/MDR1. European Journal of Pharmaceutics and Biopharmaceutics. 86(1), 74-82
• MUKHERJEE, M., LATIF, M.L., PRITCHARD, D.I. and BOSQUILLON, C., 2013. In-cell Western™ detection of organic cation transporters in bronchial epithelial cell layers cultured at an air-liquid interface on Transwell® inserts. J Pharmacol Toxicol Methods. 68(2), 184-189
• BAYARD, F.J.C., THIELEMANS, W., PRITCHARD, D.I., PAINE, S.W., YOUNG, S.S., BÄCKMAN, P., EWING, P. and BOSQUILLON, C., 2013. Polyethylene glycol-drug ester conjugates for prolonged retention of small inhaled drugs in the lung Journal of Controlled Release. 171(2), 234-240
• STEWART, C.E., TORR, E.E., MOHD JAMILI, N.H., BOSQUILLON, C. and SAYERS, I., 2012. Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research Journal of Allergy. 2012, 943982
• MUKHERJEE M, PRITCHARD DI and BOSQUILLON C, 2012. Evaluation Of Air-Interfaced Calu-3 Cell Layers For Investigation Of Inhaled Drug Interactions With Organic Cation Transporters In Vitro. International Journal Of Pharmaceutics. 426(1-2), 7-14
• REDHEAD, M., MANTOVANI, G., NAWAZ, S., CARBONE, P., GORECKI, D.C., ALEXANDER, C. and BOSQUILLON, C., 2012. Relationship between the affinity of PEO-PPO-PEO block copolymers for biological membranes and their cellular effects Pharmaceutical Research. 29(7), 1908-1918
• NAWAZ, S., REDHEAD, M., MANTOVANI, G., ALEXANDER, C., BOSQUILLON, C. and CARBONE, P., 2012. Interactions of PEO-PPO-PEO block copolymers with lipid membranes: a computational and experimental study linking membrane lysis with polymer structure Soft Matter. 8(25), 6744-6754
• HUTTER V, HILGENDORF C, COOPER A, ZANN V, PRITCHARD DI and BOSQUILLON C, 2012. Evaluation of layers of the rat airway epithelial cell line rl-65 for permeability screening of inhaled drug candidates. European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences. 47(2), 481-9
• IEONG, N.S., REDHEAD, M., BOSQUILLON, C., ALEXANDER, C., KELLAND, M. and O'REILLY, R.K., 2011. The missing lactam-thermoresponsive and biocompatible poly(N-vinylpiperidone) polymers by xanthate-mediated RAFT polymerization Macromolecules. 44(4), 886-893
• RYMER, S.J., TENDLER, S.J.B., BOSQUILLON, C., WASHINGTON, C and ROBERTS, C.J.R., 2011. Self-assembling peptides and their potential applications in biomedicine Therapeutic Delivery. 2(8), 1043-1056
• MUKHERJEE, M, PRITCHARD, DI and BOSQUILLON, C, 2011. Variations in the expression of Organic Cation Transporters (OCT) in bronchial epithelial cells following exposure to house dust components In: Annual Meeting of the British-Society-for-Allergy-and-Clinical-Immunology (BSACI). 1832-1832
• AL GHANAMI, R.C., SAUNDERS, B.R., BOSQUILLON, C., SHAKESHEFF, K.M. and ALEXANDER, C., 2010. Responsive particulate dispersions for reversible binding and deconstruction of 3D cell environments Soft Matter. 6(20), 5037-5044
• BOSQUILLON CYNTHIA, 2010. Assessing the Role of Transporters in the Disposition of Inhaled Drugs Using a Respiratory Epithelial Cell Culture Model Proceedings of the Respiratory Drug Delivery Conference. 1, 83-90
• BOSQUILLON, CYNTHIA, 2010. Drug transporters in the lung--do they play a role in the biopharmaceutics of inhaled drugs? Journal of pharmaceutical sciences. 99(5), 2240-55
• DOS SANTOS, MA, BOSQUILLON, C, RUSSOMANO, T, SUNDARESAN, A, FALCAO, F, MARRIOTT, C and FORBES, B, 2010. Modelling The Effects Of Microgravity On The Permeability Of Air Interface Respiratory Epithelial Cell Layers Advances In Space Research. 46(6), 712-718
• MUKHERJEE, M, CALANDE, T, CHEUNG, J, PRITCHARD, DI and BOSQUILLON, C, 2010. Organic Cation Transporters are Functional in Layers of the Human Bronchial Epithelial Cell Line Calu-3 Journal of Pharmacy & Pharmacology. 62, 1289-1290
• HUTTER, V, HILGENDORF, C, BROWN, A, ZANN, V, COOPER, A, PRITCHARD, D and BOSQUILLON, C, 2010. Characterisation of ATP-binding cassette (ABC) transporters in the Calu-3 human bronchial epithelial cell culture model Journal of Pharmacy & Pharmacology. 62, 1225-1226
• HUTTER, V, TANG, N, ZANN, V, COOPER, A, PRITCHARD, DI and BOSQUILLON, C, 2010. Evaluation of the rat airway epithelial RL65 cell line as a novel in-vitro model for screening inhaled drug candidates Journal of Pharmacy & Pharmacology. 62, 1230-1232
• BAYARD, F, CALANDE, T, PRITCHARD, DI, THIELEMANS, W, YOUNG, S, PAINE, S and BOSQUILLON, C, 2010. Esterase activity in the Human Bronchial Epithelial Calu-3 Cell Line Journal of Pharmacy & Pharmacology. 62, 1316-1317
• MADLOVA, MICHAELA, BOSQUILLON, CYNTHIA, ASKER, DAN, DOLEZAL, PAVEL and FORBES, BEN, 2009. In-vitro respiratory drug absorption models possess nominal functional P-glycoprotein activity. Journal of Pharmacy and Pharmacology. 61(3), 293-301
• SALEM, L.B., BOSQUILLON, C., DAILEY, L.A., DELATTRE, L., MARTIN, G.P., EVRARD, B. and FORBES, B., 2009. Sparing methylation of β-cyclodextrin mitigates cytotoxicity and permeability induction in respiratory epithelial cell layers in vitro Journal of Controlled Release. 136(2), 110-116
• MADLOVA, M., BOSQUILLON, C. and FORBES, B., 2007. P-Glycoprotein inhibitor, GF120918A, does not affect substrate absorption in the isolated perfused rat lung AEROSOLS -CONFERENCE- AEROSOL SOCIETY-. Conf 18th, 274-277
• BOSQUILLON, C., MADLOVA, M., CLEAR, N. and FORBES, B., 2007. A Simple Ex-vivo Lung Model for Screening Inhaled Drug Products AEROSOLS -CONFERENCE- AEROSOL SOCIETY-. Conf 18th, 13-16
• PATEL, J., SALEM, L. B., MARTIN, G. P., DELATTRE, L., EVRARD, B., FORBES, B. and BOSQUILLON, C., 2006. Use of the MTT assay to evaluate the biocompatibility of beta-cyclodextrin derivatives with respiratory epithelial cells JOURNAL OF PHARMACY AND PHARMACOLOGY. VOL 58(SUPP/1), 172
• BOSQUILLON, C., PRÉAT, V. and VANBEVER, R., 2004. Pulmonary delivery of growth hormone using dry powders and visualization of its local fate in rats Journal of Controlled Release. 96(2), 233-244
• BOSQUILLON, C., ROUXHET, P.G., AHIMOU, F., SIMON, D., CULOT, C., PRÉAT, V. and VANBEVER, R., 2004. Aerosolization properties, surface composition and physical state of spray-dried protein powders Journal of Controlled Release. 99(3), 357-367
• LOMBRY, C., BOSQUILLON, C., PRÉAT, V. and VANBEVER, R., 2002. Confocal imaging of rat lungs following intratracheal delivery of dry powders or solutions of fluorescent probes Journal of Controlled Release. 83(3), 331-341
• BOSQUILLON, C., LOMBRY, C., PRÉAT, V. and VANBEVER, R., 2001. Influence of formulation excipients and physical characteristics of inhalation dry powders on their aerosolization performance Journal of Controlled Release. 70(3), 329-339
• BOSQUILLON, C, LOMBRY, C, PREAT, V and VANBEVER, R, 2001. Comparison of particle sizing techniques in the case of inhalation dry powders. Journal of Pharmaceutical Sciences. 90(12), 2032-41