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From: Malcolm Richardson
To: "'MARTIN LUCK'"
Subject: RE: Matrigel +/-
Date: Wed, 14 Jan 1998 17:12:11 -0000
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From: MARTIN LUCK[SMTP:sbzml@sbn3.phes.nottingham.ac.uk]
Sent: Tuesday, January 13, 1998 1:03 PM
To: ovary-science@nottingham.ac.uk
Subject: Matrigel +/-
In reply to above. It is true that Becton Dickinson do market a growth =
factor-reduced matrigel. I bought and used some several years ago and =
the values they gave at that time were
parameter matrigel complete gf-reduced
bFGF(pg/ml) 0-0.1 0-0.1
EGF(ng/ml) 0.5-1.3 <0.5
IGF-1(ng/ml) 15.6 5
PDGF(pg/ml) 12 <5
NGF(ng/ml) <0.2 <0.2
TGF-beta(ng/ml) 2.3 1.7
The overall morphology of the clusters of granulosa cells seemed to be =
very similar on the two sorts of matrigel. We did not do detailed =
comparisons. =20
You may be interested in a paper by Aharoni et al (Current Biology vol =
7, 43-51) where they have used an FGF free matrix constructed through a =
cell line that makes matrix but not FGF. Here they did find important =
differences between FGF free and complete matrix.
Best wishes=20
Malcolm Richardson =20
5
PDGF(pg/ml) 12 <5
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F vj ¯E \à F ì ¯E'\ÁF B Albertus Extra Bold interested in a paper by Aharoni et al (Current Biology vol =
7, 43-51) where they have used an FG