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Biography
Having completed a Degree in Biochemistry, a Master in Environmental sciences, Chemistry and Health, and a PhD in Chemistry, I left the science for a couple of years and focused on its management. However my nurturing spirit brought me back into Research and Teaching by taking a research technical position at the School of Life Sciences at the Queen Medical Centre in Nottingham.
Expertise Summary
I am working at understanding the organization, regulation and function of genes. My technical expertise is based around Molecular Biology/Biochemistry. At the gene level, I can perform sub-cloning, site-directed mutagenesis, genotyping, gene expression. At the transcript level, utilizing cultured cells, I have recently trained to run and analyze qPCR experiments using common software packages. At the protein level, I am able to achieve protein/antibody purification, cell fractionation, immunoblotting, immunoprecipitation, immunocytochemistry, whole in situ hybridisation, bench flow cytometry. I am responsible for ensuring the running and safety of two busy teams, which includes teaching undergraduate and post-graduate students the basics of experimenting in a laboratory.
Teaching Summary
- Teaching of practical courses at the School of Pharmacy, University of Nottingham.
- Demonstration in the laboratory for UG/Msci/MedSci/PhD students.
- Participated to the Associate Teaching Program (ATP), awarded a Associate Fellow HEA.
Research Summary
Cancer and Heart Development.
Recent Publications
DUNAJOVÁ L, CASH E, MARKUS R, ROCHETTE S, TOWNLEY AR and WHEATLEY SP, 2016. The N-terminus of survivin is a mitochondrial-targeting sequence and Src regulator. Journal of cell science. 129(14), 2707-12 MILLAN-CALENTI J.C., TUBIO J., PITA-FERNANDEZ S., ROCHETTE S., LORENZO T. and MASEDA A, 2012. Cognitive impairment as predictor of functional dependence in an elderly sample. Arch Gerontol Geriatr.. MILLÁN-CALENTI JC, MASEDA A, ROCHETTE S and GARCÍA-MONASTERIO I, 2011. Relationship between sensory hearing loss and depression in elderly people: a literature review Rev Esp Geriatr Gerontol. 46(1), 30-5
Past Research
- School of Biosciences, Birmingham, England
Studies of cellular events after an incompatible response in the poppy pollen.
- Bayer Cropscience, Lyon, France
Development of a molecular tool, based on gene expression inhibition by RNA interference, to detect essential genes in the pathogenic rice fungus Magnaporthe grisea.
- INSERM, Marseille, France
Analysing gene expression profile by microarray in normal human melanocytes.
- Centre for Biomolecular Science, Nottingham, England
Structural studies of an enzyme with antibiotic properties.
- Grupo de investigación en Gerontología, A Coruña, Spain
Investigation of the factors associated to cognitive deterioration due to ageing so that to define the needs and improve the welfare resources of the elder ones.
- Queen Medical Center, Biomedical Sciences, Nottingham, England.
Working for Dr Sally Wheatley and Dr Siobhan Loughna