John Brameld

Contact

• workRoom B29 North Laboratory
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• work0115 951 6133
• john.brameld@nottingham.ac.uk

Biography

Professor John Brameld

Professor of Nutritional Biochemistry

John's research interests fall into three main areas: molecular nutrition, development of body composition and regulation of energy balance. Current research projects include:

• Effects of vitamin D on cell growth, metabolism and gene expression.
• Regulation of tissue (muscle, fat and liver) growth and metabolism, combining metabolomics, transcriptomics and systems biology.
• Novel regulators of energy expenditure and appetite.
• Alternative protein sources and gut digestibility, including in vitro digestion models

Expertise Summary

Skeletal muscle; adipose tissue; liver; hypothalamus; metabolism; growth; energy expenditure; appetite.

Gene expression; microarrays; transcriptomics.

In vitro and in vivo studies.

Teaching Summary

I am the module convenor for the 2nd year undergraduate module, BIOS2036 (Nutrition, metabolism & disease), and the MSc modules BIOS4069/ 4089 (Fundamentals of Nutrition) and BIOS4068 (Nutrition… read more

Research Summary

My Research interests are predominantly the study of interactions between hormones and nutrients in the regulation of growth, development and metabolism, particularly of liver, skeletal muscle and… read more

Selected Publications

• HAWKEY, KERENSA J., LOPEZ-VISO, CARLOS, BRAMELD, JOHN M., PARR, TIM and SALTER, ANDREW M., 2021. Insects: A Potential Source of Protein and Other Nutrients for Feed and Food ANNUAL REVIEW OF ANIMAL BIOSCIENCES, VOL 9, 2021. 9, 333-354
• ALLIBAND, KATHRYN H, KOZHEVNIKOVA, SOFIA V, PARR, TIM, JETHWA, PREETI H and BRAMELD, JOHN M, 2021. In vitro Effects of Biologically Active Vitamin D on Myogenesis: A Systematic Review. Frontiers in physiology. 12, 736708
• BREARLEY-SHOLTO, MADELAINE C., LOCZENSKI-BROWN, DAVID M., JONES, SARAH, DANIEL, ZOE C. T. R., EBLING, FRANCIS J. P., PARR, TIM and BRAMELD, JOHN M., 2021. Effect of AAV-mediated overexpression of ATF5 and downstream targets of an integrated stress response in murine skeletal muscle SCIENTIFIC REPORTS. 11(1),
• YIN Q, BRAMELD JM, PARR T and MURTON AJ, 2020. Leucine and mTORc1 act independently to regulate 2-deoxyglucose uptake in L6 myotubes. Amino acids. 52(3), 477-486

• View all publications

I am the module convenor for the 2nd year undergraduate module, BIOS2036 (Nutrition, metabolism & disease), and the MSc modules BIOS4069/ 4089 (Fundamentals of Nutrition) and BIOS4068 (Nutrition & metabolism).

I teach on the following modules throughout the 3 years of the undergraduate BSc degree courses:

1. BIOS1062 - Fundamentals in Food Science and Nutrition
2. BIOS1067 - Sustainable Agriculture, Food and Nutrition
3. BIOS2036 - Nutrition, Metabolism & Disease
4. BIOS2038 - Nutritional Regulation, Physiology and Endocrinology
5. BIOS2034 - Principles of Animal Nutrition
6. BIOS3029 - Animal Nutrition
7. BIOS3030 - Molecular Nutrition

I also teach on the following MSc Nutritional Sciences/ Animal Nutrition modules:

1. BIOS4069/ BIOS4087 Fundamentals of Nutrition
2. BIOS4068 Nutrition and Metabolism
3. BIOS4067/ BIOS4088 Molecular Nutrition

Current Research

My Research interests are predominantly the study of interactions between hormones and nutrients in the regulation of growth, development and metabolism, particularly of liver, skeletal muscle and adipose tissue. I previously spent some time in the pharmaceutical industry and this has influenced the direction of my studies, which fall into 3 broad areas:

1) Molecular Nutrition.

These are all cell culture-based studies investigating the mechanisms by which specific nutrients influence gene expression.

• Past studies investigated the effects of glucose and amino acids on expression of genes of the growth hormone-insulin like growth factor (GH-IGF) axis. We demonstrated interactions between various hormones (insulin, thyroid hormones, glucocorticoids and growth hormone) and specific nutrients (glucose and individual amino acids) in the regulation of expression of growth hormone receptor (GHR) and IGF-I mRNA using cultured pig hepatocytes.
• Other studies in this area include i) effects of fatty acids on expression of key fat metabolism genes (e.g. stearoyl coA desaturase, SCD); ii) effects of plant-derived polyphenols (e.g. flavonoids) on vascular endothelial cell growth and gene expression, including microarray analyses; and iii) effects of fatty acids and oxidants/antioxidants on proliferation and differentiation of cultured muscle cells.
• More recent studies are investigating the effects of nuclear hormones/vitamins (e.g. vitamin A and D) on gene expression in different cell types, as well as effects on differentiation of muscle cells, including the conversion (so called trans-differentiation) to other cell types (e.g. adipose and bone).

2) Development of body composition.

The GH-IGF axis plays a key role in regulating the growth and differentiation of virtually all cell types, but particularly skeletal muscle and adipose tissue. The effects of nutrients on the GH-IGF axis may be particularly important during fetal life when skeletal muscle (myoblast/myofibre) and fat cell (preadipocyte/adipocyte) determination, proliferation and/or differentiation takes place.

• Previous studies investigated the effects of maternal nutrition on muscle and fat cell growth and development, and the long-term consequences for growth, development and metabolism of the offspring.
• Of particular interest has been the effect of maternal nutrition on body composition, muscle fibre number and fibre type composition of the offspring, combining molecular and histochemical methodologies in a variety of animal model systems.
• This was extended to investigate the regulation of skeletal muscle fibre type transitions during postnatal development, using a combined transcriptomic and metabolomic approach. This work has been in collaboration with a major pharmaceutical company.

3) Energy balance and efficiency.

The regulation of energy balance (intake vs expenditure) and nutrient partitioning is important both in terms of body composition and efficiency of growth in farmed animals, but also humans developing obesity and associated co-morbidities (insulin resistance, hypertension, etc).

• In vitro studies include the effects of hormones and nutrients (e.g. glucose and polyphenols) on fat breakdown (lipolysis) using cultured fat explants from pigs, rats and humans.
• In vivo studies investigate the regulation of appetite and whole body energy expenditure (oxygen consumption) using closed- and open-circuit calorimeters (e.g. CLAMS), including the effects of hypothalamic neuropeptides such as TRH, AgRP and VGF, as well as dietary polyphenols and plant extracts. The role of skeletal muscle characteristics (e.g. fibre number and type) in whole body energy expenditure is of particular interest, including the peripheral and central effects of nuclear hormone receptors and associated ligands (e.g. thyroid hormone, vitamin A and D and PPARs).

4) Alternative protein sources and gut digestibility

As part of the Future Protein Platform, we have been evaluating the nutritional value and digestibility of various plant (e.g. Winged Bean and Bambara Groundnut), single cell proteins (e.g. methanogenic bacteria) and insects (e.g. mealworms, crickets and black soldier fly larvae).

• Nutritional value includes protein, energy and fat contents, amino acid and fatty acid profiles and mineral contents, as well as levels of anti-nutritional factors (ANFs), such as phytate, tannins, polyphenols and digestive enzyme. inhibitors.
• The INFOGEST in vitro digestion model is used to evaluate protein and amino acid digestibilities, including comparisons with in vivo measurements

• ALLIBAND, KATHRYN H, KOZHEVNIKOVA, SOFIA V, PARR, TIM, JETHWA, PREETI H and BRAMELD, JOHN M, 2021. In vitro Effects of Biologically Active Vitamin D on Myogenesis: A Systematic Review. Frontiers in physiology. 12, 736708
• BREARLEY-SHOLTO, MADELAINE C., LOCZENSKI-BROWN, DAVID M., JONES, SARAH, DANIEL, ZOE C. T. R., EBLING, FRANCIS J. P., PARR, TIM and BRAMELD, JOHN M., 2021. Effect of AAV-mediated overexpression of ATF5 and downstream targets of an integrated stress response in murine skeletal muscle SCIENTIFIC REPORTS. 11(1),
• BREARLEY, MADELAINE C., LOCZENSKI-BROWN, DAVID M., LOUGHNA, PAUL T., PARR, TIM and BRAMELD, JOHN M., 2021. Response of the porcine MYH4-promoter and MYH4-expressing myotubes to known anabolic and catabolic agents in vitro BIOCHEMISTRY AND BIOPHYSICS REPORTS. 25, 100924
• HAWKEY, KERENSA J., LOPEZ-VISO, CARLOS, BRAMELD, JOHN M., PARR, TIM and SALTER, ANDREW M., 2021. Insects: A Potential Source of Protein and Other Nutrients for Feed and Food ANNUAL REVIEW OF ANIMAL BIOSCIENCES, VOL 9, 2021. 9, 333-354
• DALE, T., HANNAY, I, BEDFORD, M. R., TUCKER, G. A., BRAMELD, J. M. and PARR, T., 2020. The effects of exogenous xylanase supplementation on thein vivogeneration of xylooligosaccharides and monosaccharides in broilers fed a wheat-based diet BRITISH POULTRY SCIENCE. 61(4), 471-481
• BREARLEY, MADELAINE C., DANIEL, ZOE C. T. R., LOUGHNA, PAUL T., PARR, TIM and BRAMELD, JOHN M., 2020. The phosphoenolpyruvate carboxykinase (PEPCK) inhibitor, 3-mercaptopicolinic acid (3-MPA), induces myogenic differentiation in C2C12 cells SCIENTIFIC REPORTS. 10(1), 22177
• YIN Q, BRAMELD JM, PARR T and MURTON AJ, 2020. Leucine and mTORc1 act independently to regulate 2-deoxyglucose uptake in L6 myotubes. Amino acids. 52(3), 477-486
• BREARLEY MC, LI CC, DANIEL ZCTR, LOUGHNA PT, PARR T and BRAMELD JM, 2019. Changes in expression of serine biosynthesis and integrated stress response genes during myogenic differentiation of C2C12 cells Biochemistry and Biophysics Reports. 20, 100694
• DALE, TOM N., PARR, TIM, KING, JULIE and BRAMELD, JOHN, 2019. Effects of exogenous fibrolytic enzymes on the release of xylo-oligosaccharides from six varieties of wheat in-vitro JOURNAL OF ANIMAL SCIENCE. 97, 360-361
• LOCZENSKI-BROWN DM, JONES S, LUCKETT J, DANIEL Z, BREARLEY MC, EBLING FJP, PARR T and BRAMELD JM, 2019. Effect of adeno-associated virus (AAV)-mediated overexpression of PEPCK-M (Pck2) on Clenbuterol-induced muscle growth. PloS one. 14(6), e0218970
• MACFADYEN MA, DANIEL Z, KELLY S, PARR T, BRAMELD JM, MURTON AJ and JONES SW, 2019. The commercial pig as a model of spontaneously-occurring osteoarthritis. BMC musculoskeletal disorders. 20(1), 70
• HUGGETT, Z. J., BRAMELD, J. M., BENNETT, A. J. and SALTER, A. M., 2019. Comparison of primary human hepatocytes and HepG2 cells as models to study the development of hepatic steatosis PROCEEDINGS OF THE NUTRITION SOCIETY. 78(OCE1), E14-E14
• SLINGER KR, STEWART AH, DANIEL ZCTR, HALL H, MASEY O'NEILL HV, BEDFORD MR, PARR T and BRAMELD JM, 2019. The association between faecal host DNA or faecal calprotectin and feed efficiency in pigs fed yeast-enriched protein concentrate. Animal : an international journal of animal bioscience. 13(11), 2483-2491
• BROWN DM, JONES S, DANIEL ZCTR, BREARLEY MC, LEWIS JE, EBLING FJP, PARR T and BRAMELD JM, 2018. Effect of sodium 4-phenylbutyrate on Clenbuterol-mediated muscle growth. PloS one. 13(7), e0201481
• BROWN D, RYAN K, DANIEL Z, MAREKO M, TALBOT R, MORETON J, GILES TCB, EMES R, HODGMAN C, PARR T and BRAMELD JM, 2018. The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program. Scientific reports. 8(1), 15915
• ALSHAMMARI G, KHAN R, BRAMELD J, AMER S and LOMAX MA, 2017. Gene expression of inflammatory markers in adipose tissue between obese women with polycystic ovary and normal obese women. European review for medical and pharmacological sciences. 21(5), 1099-1105
• MOHAMMED, RABEEA HAZIM, ANDERTON, HELEN, BRAMELD, JOHN MICHAEL and SWEETMAN, DYLAN, 2017. Effects of insulin like growth factors on early embryonic chick limb myogenesis PLOS ONE. 12(10),
• BRAMELD, JOHN M., BROWN, DAVID M. and PARR, TIM, 2017. The use of growth promoters in pig nutrition ACHIEVING SUSTAINABLE PRODUCTION OF PIG MEAT, VOL 2: ANIMAL BREEDING AND NUTRITION. 24, 231-254
• LEWIS JE, BRAMELD JM, HILL P, COCCO C, NOLI B, FERRI GL, BARRETT P, EBLING FJ and JETHWA PH, 2017. Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain. PloS one. 12(2), e0172724
• BROWN, DM, WILLIAMS, H, RYAN, KJP, WILSON, TL, DANIEL, ZCTR, MAREKO, MHD, EMES, RD, HARRIS, DW, JONES, S, WATTIS, JAD, DRYDEN, IL, HODGMAN, TC, BRAMELD, JM & PARR, T, 2016. Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth. Scientific Reports. 6, 28693
• BRAMELD, JOHN M. and PARR, TIM, 2016. Improving efficiency in meat production PROCEEDINGS OF THE NUTRITION SOCIETY. 75(3), 242-246
• LEWIS JE, BRAMELD JM, HILL P, WILSON, D, BARRETT P, EBLING FJP and JETHWA PH, 2016. Thyroid hormone and vitamin D regulate VGF expression and promoter activity. Journal of Molecular Endocrinology. 56(2), 123-134
• PARR T, MAREKO MH, RYAN KJ, HEMMINGS KM, BROWN DM and BRAMELD JM, 2016. The impact of growth promoters on muscle growth and the potential consequences for meat quality. Meat science. 120, 93-9
• SINCLAIR, KD, RUTHERFORD, KMD, WALLACE, JM, BRAMELD, JM and ET AL., 2016. Epigenetics and developmental programming of welfare and production traits in farm animals. Reproduction, fertility, and development. 28(10), 1443-1478
• JING, LU, HOU, YE, WU, HUI, MIAO, YUANXIN, LI, XINYUN, CAO, JIANHUA, BRAMELD, JOHN MICHAEL, PARR, TIM and ZHAO, SHUHONG, 2015. Transcriptome analysis of mRNA and miRNA in skeletal muscle indicates an important network for differential Residual Feed Intake in pigs SCIENTIFIC REPORTS. 5, 11953
• PARR, T., AL-DOSKI, S., HEMMINGS, K., DANIEL, Z., BROWN, D., LU, C., HODGMAN, C., MAY, S. and BRAMELD, J., 2015. Increased expression of serine biosynthetic pathway genes is associated with skeletal muscle hypertrophy in sheep PROCEEDINGS OF THE NUTRITION SOCIETY. 74(OCE2), E183-E183
• AL-HARBI, L., BRAMELD, J. M. and PARR, T., 2015. The active form of vitamin D, Calcitriol, induces vasodilation in the rat aorta PROCEEDINGS OF THE NUTRITION SOCIETY. 74(OCE5), E293-E293
• MAY, K., O'SULLIVAN, S. E., BRAMELD, J. M., O'NEILL, H. V. MASEY, PARR, T. and WISEMAN, J., 2015. Xylanase supplementation in feed reduces incretin and PYY levels in piglets PROCEEDINGS OF THE NUTRITION SOCIETY. 74(OCE5), E294-E294
• LEWIS JE, BRAMELD JM, HILL P, BARRETT P, EBLING FJP and JETHWA PH, 2015. The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo. Journal of neuroscience methods. 256, 22-29
• HEMMINGS, K. M., DANIEL, Z. C. T. R., BUTTERY, P. J., PARR, T. and BRAMELD, J. M., 2015. Differential effects of short-term beta agonist and growth hormone treatments on expression of myosin heavy chain IIB and associated metabolic genes in sheep muscle ANIMAL. 9(2), 285-294
• LEWIS JE, BRAMELD JM and JETHWA PH, 2015. Neuroendocrine Role for VGF. Frontiers in endocrinology. 6, 3
• BROWN DM, BRAMELD JM and PARR T, 2014. Expression of the myosin heavy chain IIB gene in porcine skeletal muscle: the role of the CArG-Box promoter response element. PloS one. 9(12), e114365
• MURPHY, M., SAMMS, R., WARNER, A., BOLBOREA, M., BARRETT, P., FOWLER, M.J., BRAMELD, J.M., TSINTZAS, K., KHARITONENKOV, A., ADAMS, A.C., COSKUN, T. and EBLING, F.J.P., 2013. Increased responses to the actions of fibroblast growth factor 21 on energy balance and body weight in a seasonal model of adiposity Journal of Neuroendocrinology. 25(2), 180-189
• RYAN, KJP, DANIEL, ZCTR, CRAGGS, LJL, PARR, T AND BRAMELD, JM, 2013. Dose-dependent effects of Vitamin D on transdifferentiation of muscle to adipose cells.: Conversion of muscle to fat cells by vitamin D Journal of Endocrinology. 217(1), 45-58
• BROWN DM, PARR T and BRAMELD JM, 2012. Myosin Heavy Chain mRNA Isoforms Are Expressed In Two Distinct Cohorts During C2C12 Myogenesis. Journal Of Muscle Research And Cell Motility. 32(6), 383-390
• MURPHY, M., JETHWA, P.H., WARNER, A., BARRETT, P., NILAWEERA, K.N., BRAMELD, J.M. and EBLING, F.J.P., 2012. Effects of manipulating hypothalamic triiodothyronine concentrations on seasonal body weight and torpor cycles in Siberian hamsters Endocrinology. 153(1), 101-112
• REHFELDT, C, PAS, MFWT, WIMMERS, K, BRAMELD, JM, NISSEN, PM, BERRI, C, VALENTE, LMP, POWER, DM, PICARD, B, STICKLAND, NC and OKSBJERG, N, 2011. Advances In Research On The Prenatal Development Of Skeletal Muscle In Animals In Relation To The Quality Of Muscle-Based Food. I. Regulation Of Myogenesis And Environmental Impact Animal. 5(5), 703-717
• REHFELDT, C, PAS, MFWT, WIMMERS, K, BRAMELD, JM, NISSEN, PM, BERRI, C, VALENTE, LMP, POWER, DM, PICARD, B, STICKLAND, NC and OKSBJERG, N, 2011. Advances In Research On The Prenatal Development Of Skeletal Muscle In Animals In Relation To The Quality Of Muscle-Based Food. Ii - Genetic Factors Related To Animal Performance And Advances In Methodology Animal. 5(5), 718-730
• BRAMELD, J M, HOLD, R and BROUGHTON PIPKIN, F, 2011. Regional variation in angiotensin converting enzyme activity in the human placenta. Placenta. 32(11), 906-908
• GRAHAM, N.S., MAY, S.T., DANIEL, Z.C.T.R., EMMERSON, Z.F., BRAMELD, J.M. and PARR, T., 2011. Use of the Affymetrix Human GeneChip array and genomic DNA hybridisation probe selection to study ovine transcriptomes Animal. 5(6), 861-866
• ZULKIFLI, R.M., PARR, T., SALTER, A.M. and BRAMELD, J.M., 2010. Regulation of ovine and porcine stearoyl coenzyme A desaturase gene promoters by fatty acids and sterols. Journal of Animal Science. 88(8), 2565-75
• JETHWA, P.H., WARNER, A., FOWLER, M.J., MURPHY, M., DE BACKER, M.W., ADAN, R.A.H., BARRETT, P., BRAMELD, J.M. and EBLING, F.J.P., 2010. Short-days induce weight loss in Siberian hamsters despite overexpression of the agouti-related peptide gene. Journal of Neuroendocrinology. 22(6), 564-575
• WARNER, A., JETHWA, P.H., WYSE, C.A., I'ANSON, H., BRAMELD, J.M. and EBLING, F.J.P., 2010. Effects of photoperiod on daily locomotor activity, energy expenditure and feeding behavior in a seasonal mammal American Journal of Physiology: Regulatory, Integrative and Comparative Physiology. 298(5), R1409-R1416
• NICHOLSON, S.K., TUCKER, G.A. and BRAMELD, J.M., 2010. Physiological concentrations of dietary polyphenols regulate vascular endothelial cell expression of genes important in cardiovascular health British Journal of Nutrition. 103(10), 1398-1403
• HEMMINGS, K.M., PARR, T., DANIEL, Z.C.T.R., PICARD, B., BUTTERY, P.J. and BRAMELD, J.M., 2009. Examination of myosin heavy chain isoform expression in ovine skeletal muscles Journal of Animal Science. 87(12), 3915-3922
• BRAMELD, J.M. and DANIEL, Z.C.T.R., 2008. In utero effects on livestock muscle development and body composition Australian Journal of Experimental Agriculture. 48(6-7), 921-929
• NICHOLSON, SONJA K, TUCKER, GREGORY A and BRAMELD, JOHN M, 2008. Effects of dietary polyphenols on gene expression in human vascular endothelial cells. Proceedings of the Nutrition Society. 67(1), 42-7
• SCHUHLER, S., WARNER, A., FINNEY, N, BENNETT, G.W., EBLING, F.J.P. and BRAMELD, J.M., 2007. Thyrotrophin-releasing hormone decreases feeding and increases body temperature, activity and oxygen consumption in Siberian hamsters. Journal of Neuroendocrinology. 19(4), 239-49
• JETHWA, P.H., WARNER, A., NILAWEERA, K.N., BRAMELD, J.M., KEYTE, J.W., CARTER, W.G., BOLTON, N., BRUGGRABER, M., MORGAN, P.J., BARRETT, P. and EBLING, F.J.P., 2007. VGF-derived peptide, TLQP-21, regulates food intake and body weight in Siberian hamsters Endocrinology. 148(8), 4044-4055
• MALLINSON, J.E., SCULLEY, D.V., CRAIGON, J., PLANT, R., LANGLEY-EVANS, S.C. and BRAMELD, J.M., 2007. Fetal exposure to a maternal low-protein diet during mid-gestation results in muscle-specific effects on fibre type composition in young rats British Journal of Nutrition. 98(2), 292-299
• DANIEL, Z. C. T. R., BRAMELD, J. M., CRAIGON, J., SCOLLAN, N. D. and BUTTERY, P. J., 2007. Effect of maternal dietary restriction during pregnancy on lamb carcass characteristics and muscle fiber composition JOURNAL OF ANIMAL SCIENCE. 85(6), 1565-1576
• DANIEL, ZCTR, BRAMELD, JM, CRAIGON, J, SCOLLAN, ND and BUTTERY, PJ, 2007. Effect Of Maternal Dietary Restriction During Pregnancy On Lamb Carcass Characteristics And Muscle Fiber Composition Journal Of Animal Science. 85(6), 1565-1576
• JETHWA, P. H., WARNER, A., BRAMELD, J. M., KEYTE, J. W., NILAWEERA, N. K., MORGAN, P. J., BARRETT, P. and EBLING, F. J., 2006. The role of a VGF derived peptide in the regulation of food intake in a seasonal rodent FRONTIERS IN NEUROENDOCRINOLOGY. VOL 27(NUMBER 1), 5-6
• BRAMELD, J., DANIEL, Z., FAHEY, A., PARR, T., SCOLLAN, N. and BUTTERY, P., 2006. Effect of maternal undernutrition on ruminant carcass and meat quality Archiv Für Tierzucht. 49(SPI), 25
• HURLEY, M.S., FLUX, C., SALTER, A.M. and BRAMELD, J.M., 2006. Effects of fatty acids on skeletal muscle cell differentiation in vitro British Journal of Nutrition. 95(3), 623-630
• FAHEY, A.J., BRAMELD, J.M., PARR, T. and BUTTERY, P.J., 2005. Ontogeny of factors associated with proliferation and differentiation of muscle in the ovine fetus Journal of Animal Science. 83(10), 2330-2338
• FAHEY, A.J., BRAMELD, J.M., PARR, T. and BUTTERY, P.J., 2005. The effect of maternal undernutrition before muscle differentiation on the muscle fiber development of the newborn lamb Journal of Animal Science. 83(11), 2564-2571
• BRAMELD, J. M., 2004. The influence of undernutrition on skeletal muscle development British Journal of Nutrition. VOL 91(PART 3), 327-328
• HEADLAND, K. R., SENSKY, P. L., BUTTERY, P. J., DICKINSON, K. and BRAMELD, J. M., 2004. Short- and long-term effects of growth hormone on basal and catecholamine-induced lipolysis in pigs PROCEEDINGS- NUTRITION SOCIETY OF LONDON. VOL 63(SPI/OCB), 100A
• PARR, T., JEWELL, K.K., SENSKY, P.L., BRAMELD, J.M., BARDSLEY, R.G. and BUTTERY, P.J., 2004. Expression of calpastatin isoforms in muscle and functionality of multiple calpastatin promoters Archives of Biochemistry and Biophysics. 427(1), 8-15
• BRAMELD, J. M. and BUTTERY, P. J., 2003. Certain amino acids regulate growth hormone (GH) binding to cultured pig hepatocytes PUBLICATION- EUROPEAN ASSOCIATION FOR ANIMAL PRODUCTION. VOL 109, 761-764
• BRAMELD JM, FAHEY AJ, LANGLEY-EVANS SC, BUTTERY PJ, 2003. Nutritional and hormonal control of muscle growth and fat deposition Archives of Animal Breeding. 46, 143-156
• FAHEY, A.J., BRAMELD, J.M., PARR, T. and BUTTERY, P.J., 2003. The effect of reducing protein and energy intake of pregnant ewes on the subsequent muscle development of the offspring. In: SOUFFRANT, W.B. and METGES, C.C., eds., Progress in research on energy and protein metabolism Wageningen: Wageningen Academic Publishers. 151-154
• SENSKY, P.L., BARDSLEY, R.G., BRAMELD, J.M., DANIEL, Z.C., FAHEY, A., JEWELL, K.K., LEE, G.K., PARR, T., SALTER, A.M., SAZILI, A.Q., BUTTERY, P.J., WISEMAN, J., VARLEY, M.A. and KEMP, B., 2003. The biochemical basis for aspects of meat quality. In: WISEMAN, J., VARLEY, M.A. and B. KEMP, eds., Perspectives in pig science Nottingham: Nottingham University Press. 133
• BRAMELD, J. M. and BUTTERY, P. J., 2002. Regulation of growth hormone (GH) binding to cultured pig hepatocytes by certain amino acids PROCEEDINGS- NUTRITION SOCIETY OF LONDON. VOL 61(SPI/OCA), 48A
• BISPHAM, J., DANDREA, J., MOSTYN, A., BRAMELD, J.M., BUTTERY, P.J., STEPHENSON, T. and SYMONDS, M.E., 2002. Impact of maternal nutrient restriction in early to mid gestation on leptin, insulin-like growth factor-1 (IGF-1) and growth hormone receptor (GHR) mRNA abundance in adipose tissue of the fetal lamb. Early Human Development. 68, 135
• BUTTERY, P.J., BRAMELD, J.M. and DAWSON, J.M., 2000. Control and manipulation of hyperplasia and hypertrophy in muscle tissue. In: CRONJÉ, P. and BOOMKER, E.A., eds., Ruminant physiology: digestion, metabolism, growth and reproduction Wallingford: CABI Publishing. 237-254
• HEASMAN, L, BRAMELD, J, MOSTVN, A, BUDGE, H, DAWSON, J, BUTTERY, P, STEPHENSON, T and SYMONDS, M E, 2000. Maternal nutrient restriction during early to mid gestation alters the relationship between insulin-like growth factor I and bodyweight at term in fetal sheep. Reproduction, fertility, and development. 12(7-8), 345-50
• BRAMELD, J.M., MOSTYN, A., DANDREA, J., STEPHENSON, T.J., DAWSON, J.M., BUTTERY, P.J. and SYMONDS, M.E., 2000. Maternal nutrition alters the expression of insulin-like growth factors in fetal sheep liver and skeletal muscle Journal of Endocrinology. 167(3), 429-37
• TAYLOR, P.M. and BRAMELD, J.M., 1999. Mechanisms and regulation of transcription and translation (Invited Review). In: Protein metabolism and nutrition: Proceedings of the VIIIth International Symposium on Protein Metabolism and Nutrition 25-50
• BRAMELD, J.M., SMAIL, H., IMRAM, N., MILLARD, N. and BUTTERY, P.J., 1999. Changes in expression of IGF-I, IGF-II and GH-receptor (GHR) mRNA during differentiation of cultured muscle cells derived from adult and fetal sheep South African Journal of Animal Science. 29 (ISRP)(Addendum), 307-310
• BRAMELD, J.M., GILMOUR, R.S. and BUTTERY, P.J., 1999. Glucose and amino acids interact with hormones to control expression of insulin-like growth factor-I and growth hormone receptor mRNA in cultured pig hepatocytes Journal of Nutrition. 129(7), 1298-1306
• BRAMELD, J.M. and BUTTERY, P.J., 1998. Effects of nutrients on the expression of IGF-1 and GH-receptor (GHR) mRNA by cultured pig hepatocytes I. Glucose Biotechnology, Agronomy Society and Environment. 2, 44
• BRAMELD, J.M. and BUTTERY, P.J., 1998. Effects of nutrients on the expression of IGF-1 and GH-receptor (GHR) mRNA by cultured pig hepatocytes II. Amino acids Biotechnology, Agronomy Society and Environment. 2, 44
• BRAMELD, J.M., BUTTERY, P.J., DAWSON, J.M. and HARPER, J.M., 1998. Nutritional and hormonal control of skeletal-muscle cell growth and differentiation In: Proceedings of the Nutrition Society. 207-217
• BRAMELD, J.M., SENSKY, P.L., BUDD, T., HARPER, J.M., PARR, T., WARD, R., WRIGHT, M., BARDSLEY, R.G., DAWSON, J., SALTER, A.M. and BUTTERY, P.J., 1998. Protein and fat metabolism in muscle and adipose tissue: its influence on meat quality. In: WISEMAN, J., VARLEY, M.A. and CHADWICK, J.P., eds., Progress in pig science Nottingham: Nottingham University Press. 443-460
• BRAMELD, J.M., 1997. Molecular mechanisms involved in the nutritional and hormonal regulation of growth in pigs In: Proceedings of the Nutrition Society. 607-619
• GUAN, X.F., BRAMELD, J.M., TROTTIER, N.L. and BUTTERY, P.J., 1997. Nutritional and hormonal regulation of insulin-like growth factor binding protein production in cultured porcine hepatocytes Journal of Animal Science. 75(Supplement 1), 77
• GUAN, X.F., BRAMELD, J.M. and BUTTERY, P.J., 1997. Effects of dietary protein levels and porcine growth hormone administration on plasma concentrations of insulin-like growth factor binding proteins in growing pigs In: Proceedings of the Nutrition Society. 168A
• BRAMELD, J.M., ATKINSON, J.L., SAUNDERS, J.C., PELL, J.M., BUTTERY, P.J. and GILMOUR, R.S., 1996. Effects of growth hormone administration and dietary protein intake on insulin-like growth factor-I and growth hormone receptor mRNA expression in porcine liver, skeletal muscle and adipose tissue Journal of Animal Science. 74, 1832-1841
• BRAMELD, J.M., ATKINSON, J.L., BUDD, T.J., SAUNDERS, J.C., PELL, J.M., SALTER, A.M., GILMOUR, R.S. and BUTTERY, P.J., 1996. Expression of insulin-like growth factor-1 (IGF-1) and growth hormone-receptor (GHR) mRNA in liver, skeletal muscle and adipose tissue of different breeds of pig Animal Science. 62(3), 555-560
• BRAMELD, J.M., WELLER, P.A., SAUNDERS, J.C., BUTTERY, P.J. and GILMOUR, R.S., 1995. Hormonal control of insulin-like growth factor-1 and growth hormone receptor mRNA expression by porcine hepatocytes in culture Journal of Endocrinology. 146, 239-245
• BUTTERY, P.J., BRAMELD, J.M. and DAWSON, J.M., 1995. Regulation of growth and carcass composition in farm animals In: Proceedings of the 2nd Dummerstorf muscle workshop (Rostock, Germany). 175-182
• BRAMELD, J.M., ATKINSON, J.L., BUDD, T.J., SAUNDERS, J.C., SALTER, A.M., GILMOUR, R.S. and BUTTERY, P.J., 1995. Expression of IGF-I and GH-receptor (GHR) mRNA in liver and skeletal muscle of different pig breeds Animal Science. 60, 561
• BRAMELD, J.M., ATKINSON, J.L., SAUNDERS, J.C., PELL, J.M., BUTTERY, P.J. and GILMOUR, R.S., 1995. Effects of GH administration and dietary protein intake on IGF-I and GH-receptor (GHR),mRNA expression in pigs (Abstract) In: Proceedings of the 2nd Dummerstorf muscle workshop (Rostock, Germany).
• BRAMELD, J.M., WELLER, P.A., PELL, J.M., BUTTERY, P.J. and GILMOUR, R.S., 1995. Ontogenic study of insulin-like growth factor-1 and growth hormone receptor mRNA expression in porcine liver and skeletal muscle Animal Science. 61, 333-339
• BLACHOWSKI, S., CHARLTON, D., BRAMELD, J., BUTTERY, P.J. and MOTYL, T., 1994. Comparison of metabolic effects of EGF, IGF-I, IGF-II and insulin in culture of fetal sheep myoblasts and fetal sheep fibroblasts. In: The developing of digestive and metabolic processes in new born and growing ruminants 69-70
• BLACHOWSKI, S., BUTTERY, P.J., BRAMELD, J., CHARLTON, D. and MOYTL, T., 1994. Inhibition of proliferation and differentiation of fetal sheep myoblasts by an inhibitor of ornithine decarboxylase-DFMO In: Proceedings of the Society of Nutrition Physiology. 291
• WELLER, P.A., DAUNCEY, M.J., BATES, P.C., BRAMELD, J.M., BUTTERY, P.J. and GILMOUR, R.S., 1994. Regulation of porcine insulin-like growth-factor-1 and growth hormone receptor-RNA expression by energy status American Journal of Physiology. 266, E776-E785
• BRAMELD, J.M., WELLER, P.A., GILMOUR, R.S. and BUTTERY, P.J., 1993. Ontogenic study of expression of IGF-I and GH-receptor (GHR) mRNA in pig liver and skeletal muscle Animal Production. 56, 457
• BRAMELD, J M, BROUGHTON PIPKIN, F and SYMONDS, E M, 1990. Studies on the human ovarian renin-angiotensin system: optimization of assay methodology and effects of follicular stimulants. Journal of Endocrinology. 127(3), 513-21
• HESSLEWOOD, S R, THORNBACK, J R and BRAMELD, J M, 1988. Indium-111 in breast milk following administration of indium-111-labeled leukocytes. The Journal of Nuclear Medicine. 29(7), 1301-2