Joëlle Goulding

Contact

• workRoom C101 The University of Nottingham Medical School
  Queen's Medical Centre
  Nottingham
  NG7 2UH
  UK
• work0115 82 30458
• joelle.goulding@nottingham.ac.uk

Biography

Joëlle Goulding (née Alcock) graduated from the University of Nottingham with a BSc (Hons) in Genetics (2001) and went on to complete a PhD within the Institute of Genetics (2005). Subsequent postdoctoral work involved investigations into adult stem cells of the cerebellum. Joëlle then joined the Cell Signalling Research Group within the University of Nottingham (2007) changing research fields and prompting a strong focus on Molecular Pharmacology. Through studies of endogenous and transgenic expression systems of the adenosine and adrenergic receptors utilising protein-tags, biosensors and fluorescent ligands, Joëlle has built up a wide technological knowledge base. In 2009 Joëlle visited the laboratory of Prof. Carsten Hoffman, Wurzberg Germany, to learn a FlAsh-based FRET technique to study GPCR activation. In 2015 she was awarded a Diploma in Advanced Pharmacology by the British Pharmacological Society. More recently Joëlle has gained an Associate Fellowship of the Higher Education Academy.

In April 2017 Joëlle took up the position of Research Fellow in Advanced Microscopy within COMPARE at Nottingham University. She will be developing research within fluorescence correlation spectroscopy alongside testing methodologies for the study of membrane receptors with the newly acquired Olympus LV200 and PhaseFocus Livecyte.

Expertise Summary

Joëlle has a passion for imaging techniques and has implemented this passion by developing and optimising strategies to study receptor function.

Imaging expertise

· Light, confocal and wide-field microscopy (Zeiss LSM 510, 710, 880, Exciter, DeltaVision Elite)

· TIRF (total internal reflection fluorescence microscopy)

· FCS (Fluorescence correlation spectroscopy)

· High-throughput microscopy (Molecular Devices Ultra and Micro confocal plate readers)

· Perfusion - as used in http://dx.doi.org/10.1124/mol.110.064493

· Data analysis - MetaXpress, Volocity, GraphPad Prism

Additional laboratory-based expertise

· Cell Signalling assays/biosensors - including cAMP, Ca²⁺, ERK; techniques including FLEX, FRET and BRET

· Cell culture - Generation of primary cell lines, transient transfection and generation of stable cell lines

· Radioligand binding, competition and accumulation assays

Teaching Summary

Main responsibilities:

Workshop Convenor

Maths in the Lab - Essential maths skills in day-to-day lab work (Graduate school course)

Key Contributor

A13GPD - GPCR polymorphisms, disease and personalised medicine (Year 3, BMedSci)

B33PPM - GPCR based personalised medicine (Year 3, MPharm)

Research Summary

Joëlle is the Microscope Officer for the Nottingham side of COMPARE and is currently developing techniques around Fluorescence Correlation Spectroscopy with particular reference to the study of… read more

Selected Publications

• STODDART, LEIGH A., JOHNSTONE, ELIZABETH K. M., WHEAL, AMANDA J., GOULDING, JOELLE, ROBERS, MATTHEW B., MACHLEIDT, THOMAS, WOOD, KEITH V., HILL, STEPHEN J. and PFLEGER, KEVIN D. G., 2015. Application of BRET to monitor ligand binding to GPCRs NATURE METHODS. 12(7), 661-663
• ALCOCK, J., WARREN, A.Y., GOODSON, Y.J., HILL, S.J., KHAN, R.N. and LYMN, J.S., 2010. Inhibition of tissue transglutaminase 2 attenuates contractility of pregnant human myometrium Biology of Reproduction. 84(4), 646-653
• ALCOCK, J. and SOTTILE, V., 2009. Dynamic distribution and stem cell characteristics of Sox1-expressing cells in the cerebellar cortex Cell Research. 19(12), 1324-1333
• ALCOCK, JOELLE, LOWE, JAMES, ENGLAND, TIM, BATH, PHILIP and SOTTILE, VIRGINIE, 2009. Expression of Sox1, Sox2 and Sox9 is maintained in adult human cerebellar cortex. Neuroscience Letters. 450(2), 114-6

• View all publications

Current Research

Joëlle is the Microscope Officer for the Nottingham side of COMPARE and is currently developing techniques around Fluorescence Correlation Spectroscopy with particular reference to the study of endogenously expressed receptors.

Check out https://www.imagingscientist.com/ ( https://www.imagingscientist.com/joelle-goulding.html ) for a feel for the role of the Imaging Scientist in todays scientific research.

Microscopy Resources:-

https://www.ibiology.org/online-biology-courses/microscopy-series/

https://www.olympus-lifescience.com/en/microscope-resource/

https://www.microscopyu.com/

http://olympus.magnet.fsu.edu/index.html

http://micro.magnet.fsu.edu/index.html

• KOO, CHEK ZIU, HARRISON, NEALE, NOY, PETER J, SZYROKA, JUSTYNA, MATTHEWS, ALEXANDRA L, HSIA, HUNG-EN, MULLER, STEPHAN A, TUSHAUS, JOHANNA, GOULDING, JOELLE, WILLIS, KATIE, APICELLA, CLARA, CRAGOE, BETHANY, DAVIS, EDWARD, KELES, MURAT, MALINOVA, ANTONIA, MCFARLANE, THOMAS A, MORRISON, PHILIP R, NGUYEN, HANH T H, SYKES, MICHAEL C, AHMED, HAROON, DI MAIO, ALESSANDRO, SEIPOLD, LISA, SAFTIG, PAUL, CULL, ELEANOR, PLIOTAS, CHRISTOS, RUBINSTEIN, ERIC, POULTER, NATALIE S, BRIDDON, STEPHEN J, HOLLIDAY, NICHOLAS D, LICHTENTHALER, STEFAN F and TOMLINSON, MICHAEL G, 2020. The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex. The Journal of biological chemistry.
• SOAVE, MARK, GOULDING, JOELLE, MARKUS, ROBERT, HILL, STEPHEN J and STODDART, LEIGH A, 2020. Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy. Methods in molecular biology (Clifton, N.J.). 2041, 163-181
• GOULDING J, MAY LT and HILL SJ, 2017. Characterisation of endogenous A2A and A2B receptor-mediated cyclic AMP responses in HEK 293 cells using the GloSensor™ biosensor: Evidence for an allosteric mechanism of action for the A2B-selective antagonist PSB 603. Biochemical pharmacology. (In Press.)
• STODDART, LEIGH A., JOHNSTONE, ELIZABETH K. M., WHEAL, AMANDA J., GOULDING, JOELLE, ROBERS, MATTHEW B., MACHLEIDT, THOMAS, WOOD, KEITH V., HILL, STEPHEN J. and PFLEGER, KEVIN D. G., 2015. Application of BRET to monitor ligand binding to GPCRs NATURE METHODS. 12(7), 661-663
• ALCOCK, J., WARREN, A.Y., GOODSON, Y.J., HILL, S.J., KHAN, R.N. and LYMN, J.S., 2010. Inhibition of tissue transglutaminase 2 attenuates contractility of pregnant human myometrium Biology of Reproduction. 84(4), 646-653
• ALCOCK, JOELLE, LOWE, JAMES, ENGLAND, TIM, BATH, PHILIP and SOTTILE, VIRGINIE, 2009. Expression of Sox1, Sox2 and Sox9 is maintained in adult human cerebellar cortex. Neuroscience Letters. 450(2), 114-6
• ALCOCK, J. and SOTTILE, V., 2009. Dynamic distribution and stem cell characteristics of Sox1-expressing cells in the cerebellar cortex Cell Research. 19(12), 1324-1333
• ALCOCK J, WARREN AY, GOODSON JY, HILL SJ, KHAN RN, LYMN JS, 2009. Oxytocin-induced contractility in human myometrium is inhibited by inhibitors of the enzyme tissue transglutaminase Reproductive Sciences. 16(3), P758
• ALCOCK J, GOODSON JY, WARREN AY, HILL SJ, KHAN RN, LYMN JS, 2009. Phospholipase C isoform expression in the human myometrium is differentially regulated in pregnancy Reproductive Sciences. 16(3), P757
• THOMSON NL, ALCOCK J, GOODSON JY, WARREN AY, HILL SJ, KHAN RN, LYMN JS, 2009. Expression and cellular localisation of tissue transglutaminase II in human myometrium Reproductive Sciences. 16(3), P802
• ALCOCK,, SCOTTING, and SOTTILE,, 2007. Bergmann glia as putative stem cells of the mature cerebellum. Medical Hypotheses.
• SOAVE M, GOULDING J, MARKUS R, HILL SJ and STODDART LA, Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy. Methods in molecular biology (Clifton, N.J.). 2041, 163-181