Sophie Rochette

Contact

• workRoom C5 Medical School
  Queen's Medical Centre
  Nottingham
  NG7 2UH
  UK
• work01158230305
• Sophie.Rochette@nottingham.ac.uk

Biography

Having completed a Degree in Biochemistry, a Master in Environmental sciences, Chemistry and Health, and a PhD in Chemistry, I left the science for a couple of years and focused on its management. However my nurturing spirit brought me back into Research and Teaching by taking a research technical position at the School of Life Sciences at the Queen Medical Centre in Nottingham.

Expertise Summary

I am working at understanding the organization, regulation and function of genes. My technical expertise is based around Molecular Biology/Biochemistry. At the gene level, I can perform sub-cloning, site-directed mutagenesis, genotyping, gene expression. At the transcript level, utilizing cultured cells, I have recently trained to run and analyze qPCR experiments using common software packages. At the protein level, I am able to achieve protein/antibody purification, cell fractionation, immunoblotting, immunoprecipitation, immunocytochemistry, whole in situ hybridisation, bench flow cytometry. I am responsible for ensuring the running and safety of two busy teams, which includes teaching undergraduate and post-graduate students the basics of experimenting in a laboratory.

Teaching Summary

- Teaching of practical courses at the School of Pharmacy, University of Nottingham.

- Demonstration in the laboratory for UG/Msci/MedSci/PhD students.

- Participated to the Associate Teaching Program (ATP), awarded a Associate Fellow HEA.

Research Summary

Cancer and Heart Development.

Recent Publications

• PARNALL, MATTHEW, PERDIOS, CHRYSOSTOMOS, PANG, KAR LAI, ROCHETTE, SOPHIE and LOUGHNA, SIOBHAN, 2019. Characterisation of the developing heart in a pressure overloaded model utilising RNA sequencing to direct functional analysis JOURNAL OF ANATOMY.
• DUNAJOVÁ L, CASH E, MARKUS R, ROCHETTE S, TOWNLEY AR and WHEATLEY SP, 2016. The N-terminus of survivin is a mitochondrial-targeting sequence and Src regulator. Journal of cell science. 129(14), 2707-12
• MILLAN-CALENTI J.C., TUBIO J., PITA-FERNANDEZ S., ROCHETTE S., LORENZO T. and MASEDA A, 2012. Cognitive impairment as predictor of functional dependence in an elderly sample. Arch Gerontol Geriatr..
• MILLÁN-CALENTI JC, MASEDA A, ROCHETTE S and GARCÍA-MONASTERIO I, 2011. Relationship between sensory hearing loss and depression in elderly people: a literature review Rev Esp Geriatr Gerontol. 46(1), 30-5

• View all publications

Past Research

- School of Biosciences, Birmingham, England

Studies of cellular events after an incompatible response in the poppy pollen.

- Bayer Cropscience, Lyon, France

Development of a molecular tool, based on gene expression inhibition by RNA interference, to detect essential genes in the pathogenic rice fungus Magnaporthe grisea.

- INSERM, Marseille, France

Analysing gene expression profile by microarray in normal human melanocytes.

- Centre for Biomolecular Science, Nottingham, England

Structural studies of an enzyme with antibiotic properties.

- Grupo de investigación en Gerontología, A Coruña, Spain

Investigation of the factors associated to cognitive deterioration due to ageing so that to define the needs and improve the welfare resources of the elder ones.

- Queen Medical Center, Biomedical Sciences, Nottingham, England.

Working for Dr Sally Wheatley and Dr Siobhan Loughna

• PARNALL, MATTHEW, PERDIOS, CHRYSOSTOMOS, PANG, KAR LAI, ROCHETTE, SOPHIE and LOUGHNA, SIOBHAN, 2019. Characterisation of the developing heart in a pressure overloaded model utilising RNA sequencing to direct functional analysis JOURNAL OF ANATOMY.
• DUNAJOVÁ L, CASH E, MARKUS R, ROCHETTE S, TOWNLEY AR and WHEATLEY SP, 2016. The N-terminus of survivin is a mitochondrial-targeting sequence and Src regulator. Journal of cell science. 129(14), 2707-12
• MILLAN-CALENTI J.C., TUBIO J., PITA-FERNANDEZ S., ROCHETTE S., LORENZO T. and MASEDA A, 2012. Cognitive impairment as predictor of functional dependence in an elderly sample. Arch Gerontol Geriatr..
• MILLÁN-CALENTI JC, MASEDA A, ROCHETTE S and GARCÍA-MONASTERIO I, 2011. Relationship between sensory hearing loss and depression in elderly people: a literature review Rev Esp Geriatr Gerontol. 46(1), 30-5
• MILLAN-CALENTI J.C., MASEDA A., ROCHETTE S., VAZQUEZ G.A. and SANCHEZ A., 2011. Mental and psychological conditions, medical comorbidity and functional limitation: differential associations in older adults with cognitive impairment, depressive symptoms and co-existence of both. 26(10), 1071-9
• BARDELANG, P., VANKEMMELBEKE, M., ZHANG, Y., JARVIS, H., ANTONIADOU, E., ROCHETTE, S., THOMAS, N.R., PENFOLD, C.N. and JAMES, R., 2009. Design of a polypeptide FRET substrate that facilitates study of the antimicrobial protease lysostaphin Biochemical Journal. 418, 615-624
• WARFIELD, RACHEL, BARDELANG, PHILIP, SAUNDERS, HELEN, CHAN, WENG C, PENFOLD, CHRISTOPHER, JAMES, RICHARD and THOMAS, NEIL R, 2006. Internally quenched peptides for the study of lysostaphin: an antimicrobial protease that kills Staphylococcus aureus. Organic and Biomolecular Chemistry. 4(19), 3626-38
• BON B., BRUNEAU N., ROCHETTE S., LOMBARDO D., 2005. Bile salt-dependent lipase could target human skin melanocytes and modify their transcriptome independently of LOX-1 receptor. Journal of Investigative Dermatology.