Simon Dawson

Contact

• workRoom D76 (within D75) University of Nottingham Medical School
  Queen's Medical Centre
  Nottingham
  NG7 2UH
  UK
• work0115 82 30121
• fax0115 82 30103
• simon.dawson@nottingham.ac.uk

Research Summary

Research Interests Research interests include the role of the gankyrin oncoprotein in hepatocellular carcinogenesis; the roll of ubiquitin-protein ligase enzymes (E3s) in cell biology particularly… read more

Recent Publications

• REZVANI, K, BAALMAN, K, TENG, Y, MEE, M.P., DAWSON, S.P., WANG, H, DE BIASI, M and MAYER, R.J., 2012. Proteasomal degradation of the metabotropic glutamate receptor 1α is mediated by Homer-3 via the proteasomal S8 ATPase: Signal transduction and synaptic transmission Journal of Neurochemistry. 122(1), 24-37
• DAWSON, S.P., 2008. Hepatocellular carcinoma and the ubiquitin-proteasome system Biochimica et Biophysica Acta: Molecular Basis of Disease. 1782(12), 775-784
• MARX, FP, SOEHN, AS, BERG, D, MELLE, C, SCHIESLING, C, LANG, M, KAUTZMANN, S, STRAUSS, KM, FRANCK, T, ENGELENDER, S, PAHNKE, J, DAWSON, S, VON EGGELING, F, SCHULZ, JB, RIESS, O and KRUGER, R, 2007. The proteasomal subunit S6 ATPase is a novel synphilin-1 interacting protein--implications for Parkinson's disease. FASEB Journal. 21(8), 1759-67
• DAWSON, S., HIGASHITSUJI, H., WILKINSON, A.J., FUJITA, J. and MAYER, R.J., 2006. Gankyrin: a new oncoprotein and regulator of pRb and p53 Trends in Cell Biology. 16(5), 229-233

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Current Research

Research Interests Research interests include the role of the gankyrin oncoprotein in hepatocellular carcinogenesis; the roll of ubiquitin-protein ligase enzymes (E3s) in cell biology particularly the NEDD4-family of E3s within intracellular protein trafficking, signalling and the identification of potential substrates thereof; ubiquitin-like proteins. Techniques • Protein-protein interaction analysis - two-hybrid screening and analysis via 'classical' and SOS/ras yeast and bacterial systems, co-immunoprecipitation, fluorescence microscopy • Cell tissue culture • General molecular biology - PCR, in vitro mutagenesis, cloning etc • Recombinant protein expression via bacterial and Pichia pastoris systems • Reporter gene analysis

• REZVANI, K, BAALMAN, K, TENG, Y, MEE, M.P., DAWSON, S.P., WANG, H, DE BIASI, M and MAYER, R.J., 2012. Proteasomal degradation of the metabotropic glutamate receptor 1α is mediated by Homer-3 via the proteasomal S8 ATPase: Signal transduction and synaptic transmission Journal of Neurochemistry. 122(1), 24-37
• DAWSON, S.P., 2008. Hepatocellular carcinoma and the ubiquitin-proteasome system Biochimica et Biophysica Acta: Molecular Basis of Disease. 1782(12), 775-784
• MARX, FP, SOEHN, AS, BERG, D, MELLE, C, SCHIESLING, C, LANG, M, KAUTZMANN, S, STRAUSS, KM, FRANCK, T, ENGELENDER, S, PAHNKE, J, DAWSON, S, VON EGGELING, F, SCHULZ, JB, RIESS, O and KRUGER, R, 2007. The proteasomal subunit S6 ATPase is a novel synphilin-1 interacting protein--implications for Parkinson's disease. FASEB Journal. 21(8), 1759-67
• DAWSON, S., HIGASHITSUJI, H., WILKINSON, A.J., FUJITA, J. and MAYER, R.J., 2006. Gankyrin: a new oncoprotein and regulator of pRb and p53 Trends in Cell Biology. 16(5), 229-233
• HIGASHITSUJI, H., HIGASHITSUJI, H., ITOH, K., SAKURAI, T., NAGAO, T., SUMITOMO, H., MASUDA, T., DAWSON, S., SHIMADA, Y., MAYER, R. J. and FUJITA, J, 2005. The oncoprotein gankyrin binds to MDM2/HDM2, enhancing ubiquitylation and degradation of p53 Cancer Cell. 8(1), 75-87
• APCHER, G-S, MAITLAND, J, DAWSON, S, SHEPPARD, P and MAYER, R, 2004. The alpha4 and alpha7 subunits and assembly of the 20S proteasome. FEBS Letters. 569(1-3), 211-216
• KRZYWDA, S., BRZOZOWSKI, A. M., HIGASHITSUJI, H., FUJITA, J., WELCHMAN, R., DAWSON, S., MAYER, R. J. and WILKINSON, A. J., 2004. The crystal structure of gankyrin, an oncoprotein found in complexes with cyclin-dependent kinase 4, a 19 S proteasomal ATPase regulator, and the tumor suppressors Rb and p53 Journal of Biological Chemistry. 279(2), 1541-1545
• REZVANI, K, MEE, M, DAWSON, S, MCILHINNEY, J, FUJITA, J and MAYER, R J, 2003. Proteasomal interactors control activities as diverse as the cell cycle and glutaminergic neurotransmission. Biochemical Society Transactions. 31(2), 470-3
• NAGAO, T., HIGASHITSUJI, H., NONOGUCHI, K., SAKURAI, T., DAWSON, S., MAYER, R. J., ITOH, K. and FUJITA, J., 2003. MAGE-A4 interacts with the liver oncoprotein gankyrin and suppresses its tumorigenic activity Journal of Biological Chemistry. 278(12), 10668-10674
• DOHERTY, F. J., DAWSON, S. and MAYER, R. J., 2002. The ubiquitin-proteasome pathway of intracellular proteolysis Essays in Biochemistry. VOL 38, 51-64
• DAWSON, S., APCHER, S., MEE, M., HIGASHITSUJI, H., BAKER, R., UHLE, S., DUBIEL, W., FUJITA, J. and MAYER, R. J., 2002. Gankyrin is an ankyrin-repeat oncoprotein that interacts with CDK4 kinase and the S6 ATPase of the 26 S proteasome Journal of Biological Chemistry. 277(13), 10893-10902
• LAYFIELD, R, TOOTH, D, LANDON, M, DAWSON, S, MAYER, J and ALBAN, A, 2001. Purification of poly-ubiquitinated proteins by S5a-affinity chromatography. Proteomics. 1(6), 773-7