Tim Self

Contact

• workRoom C89A The University of Nottingham Medical School
  Queen's Medical Centre
  Nottingham
  NG7 2UH
  UK
• work0115 82 30090
• tim.self@nottingham.ac.uk
• http://nottingham.ac.uk/life-sciences/facilities/slim/index.aspx

Biography

I have spent my professional life applying microscopy and preparation techniques to answer complex scientific and biological research problems. I have roles in academia and for the Medical Research Council where I have supported full time research projects alongside setting up and developing high end imaging facilities at each institution. I have act as advisor for Zeiss, Molecular Devices, Phasefocus, Nikon, BBSRC on a number of occasions.

Founder member of UK Light Microscope Facility Managers Group and UkBioImaging. I am also Chair of the University of Nottingham Facility Manager Group and organised the inaugural Zeiss Elyra Super Resolution user group meeting in 2017.

Expertise Summary

My expertise gained from 35 years working with microscopes in biomedical research spans a wide range of imaging and preparation techniques. I can determine and apply on the most appropriate preparation technique for samples to visualise and study at the highest resolution such as Electron Microscopy to more simple light microscopy. I have a particular skills in live cell and fluorescence imaging gained from the last 17 years building and developing the facilities and applications at University of nottingham. I have a long track record of peer reviewed publications that cover a wide range of scientific research areas.

My expertise has been instrumental in the development and implementation of a number of successful multi million pound proramme grants awarded by MRC, BBSRC, Wellcome and SRIF. I am instrumental in the application and development of novel technology to answer important scientific questions through microscopy.

Teaching Summary

I am an Associate Fellow of the HEA and with my team run regular workshops for staff, Doctoral Training Programmes, post graduate students, undergraduate student practical classes. I also lecture to… read more

Research Summary

Applying microscopy and imaging to answer complex research questions.

Developing light microscope techniques with School of Life Sciences Imaging (SLIM) to bring understanding of microscopy and research to schools and the public through outreach activities and programmes.

Selected Publications

• SELF, T, MAHONY, M, FLEMING, J, WALSH, J, BROWN, S D and STEEL, K P, 1998. Shaker-1 mutations reveal roles for myosin VIIA in both development and function of cochlear hair cells. Development (Cambridge). 125(4), 557-66
• SELF, T.J., OAKLEY, S.M. and HILL, S.J., 2005. Clathrin-independent internalization of the human histamine H1-receptor in CHO-K1 cells British Journal of Pharmacology. 146(4), 612-24
• KROS, C J, MARCOTTI, W, VAN NETTEN, S M, SELF, T J, LIBBY, R T, BROWN, S D M, RICHARDSON, G P and STEEL, K P, 2002. Reduced climbing and increased slipping adaptation in cochlear hair cells of mice with Myo7a mutations. Nature Neuroscience. 5(1), 41-7
• ORIHUELA, C.J., MAHDAVI, J., THORNTON, J., MANN, B., WOOLDRIDGE, K.G., ABOUSEADA, N., OLDFIELD, N.J., SELF, T., ALA'ALDEEN, D.A.A. and TUOMANEN, E.I., 2009. Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. Journal of Clinical Investigation. 119(6), 1638-1646

• View all publications

I am an Associate Fellow of the HEA and with my team run regular workshops for staff, Doctoral Training Programmes, post graduate students, undergraduate student practical classes. I also lecture to MSC Stem cell students on fundamentals of light and fluorescence microscopy. Post graduate teaching is delivered through a number of two day workshops and can be summarised:

The aims of the course are to introduce the students to fundamentals of microscopy, preparation and image analysis. The course will involve a mix of theory talks and hands-on workshops using state of the art microscopes. By the end of the course the students will understand the principles of fluorescence, resolution, operation of the microscope and be able to analyse images acquired on some of the systems available in Life Sciences. The two-day workshop with consist of a series of lectures followed by hands on practical sessions.

• BRAULIO MARTINEZ DE LA CRUZ, ROBERT MARKUS, SUNIR MALLA,, MARIA ISABEL HAIG, CHRIS GELL, FEI SANG, ELEANOR BELLOWS, MAHMOUD AWAD SHERIF, DENISE MCLEAN, ANBARASU LOURDUSAMY, TIM SELF, ZSUZSANNA BODI, STUART SMITH, MICHAEL FAY, IAN A. MACDONALD, RUPERT FRAY and HELEN MIRANDA KNIGHT, 2021. Modifying the m6A brain methylome by ALKBH5-mediated demethylation: a new contender for synaptic tagging Molecular Psychiatry. open,
• STEPHEN WOODHAMS, ROBERT MARKUS, PETER GOWLER and TIMOTHY SELF, 2019. Cell type-specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at peptidergic primary afferent terminals as a spinal anatomical correlate of inflammatory pain PAIN: ISAP. (In Press.)
• SEEMA RAJANI, ROBERT MARKUS, IAN WARD, DENISE MCLEAN, CHRIS GELL and TIM SELF, 2017. Build your own Raspberry pi microscope The Proceeding of the Royal microscopical Society: InFocus. June 2017
• TIM SELF and ROBERT MARKUS, 2015. Super resolution microscopy- the bright side of molecular biology Pharmacology Matters. 8(3), 5-7
• AHMED KHAIRALLA, S.OMER, J MAHDAVI, A ASLAM, O DUFAILU, TIM SELF, A JONSSON, H SJOLINDER, L MARTINEZ-POMARES, A GHAEMMAGHAMI, K WOOLDRIDGE and N OLDFIELD, 2015. Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransporters Cellular Microbiology. 17(7), 1008-1020
• MAHDAVI J, PIRINCCIOGLU N, OLDFIELD NJ, CARLSOHN E, STOOF J, ASLAM A, SELF T, CAWTHRAW SA, PETROVSKA L, COLBORNE N, SIHLBOM C, BORÉN T, WOOLDRIDGE KG and ALA'ALDEEN DAA, 2014. A novel O-linked glycan modulates Campylobacter jejuni major outer membrane protein-mediated adhesion to human histo-blood group antigens and chicken colonization Open Biology. 4, 130202
• ROSS CORRIDEN, TIM SELF, KATHRYN AKONG-MOORE, VICTOR NIZET, BARRY KELLAM and STEPHEN J BRIDDON, 2013. Adenosine-A3 receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes EMBO Reports. 14, 726-732
• MAHDAVI, J., ROYER, P.-J., SJÖLINDER, H.S., AZIMI, S., SELF, T., STOOF, J., WHELDON, L.M., BRÄNNSTRÖM, K., WILSON, R., MORETON, J., MOIR, J.W.B., SIHLBOM, C., BORÉN, T., JONSSON, A.-B., SOULTANAS, P. and ALA'ALDEEN, D.A.A., 2013. Pro-inflammatory cytokines can act as intracellular modulators of commensal bacterial virulence Open Biology. 3, 130048
• KAY, C., WOODWARD, K.D., LAWLER, K., SELF, T.J., DYALL, S.D. and KERR, I.D., 2012. The ATP-binding cassette proteins of the deep-branching protozoan parasite Trichomonas vaginalis PLoS Neglected Tropical Diseases. 6(6), e1693
• KAY C, LAWLER K, SELF TIM, DYALL S and KERR I, 2012. Localisation of a family of complex-forming β-barrels in the T. vaginalis hydrogenosomal membrane Febs Letters. 586, 4038-4045
• HAIDER, A.J., BRIGGS, D., SELF, T.J., CHILVERS, H.L., HOLLIDAY, N.D. and KERR, I.D., 2011. Dimerization of ABCG2 analysed by bimolecular fluorescence complementation PLoS ONE. 6(10), e25818
• JAMES, V., ZHANG, Y., FOXLER, D.E., DE MOOR, C.H., KONG, Y.W., WEBB, T.M., SELF, T.J., FENG, Y., LAGOS, D., CHU, C.-Y., RANA, T.M., MORLEY, S.J., LONGMORE, G.D., BUSHELL, M. and SHARP, T.V., 2010. LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing Proceedings of the National Academy of Sciences of the United States of America. 107(28), 12499-12504
• MAY, L.T., SELF, T.J., BRIDDON, S.J. and HILL, S.J., 2010. The effect of allosteric modulators on the kinetics of agonist-G protein-coupled receptor interactions in single living cells Molecular Pharmacology. 78(3), 511-523
• GHOSH, T.K., SONG, F.F., PACKHAM, E.A., BUXTON, S., ROBINSON, T.E., RONKSLEY, J., SELF, T., BONSER, A.J. and BROOK, J.D., 2009. Physical interaction between TBX5 and MEF2C is required for early heart development Molecular and Cellular Biology. 29(8), 2205-2218
• ORIHUELA, C.J., MAHDAVI, J., THORNTON, J., MANN, B., WOOLDRIDGE, K.G., ABOUSEADA, N., OLDFIELD, N.J., SELF, T., ALA'ALDEEN, D.A.A. and TUOMANEN, E.I., 2009. Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. Journal of Clinical Investigation. 119(6), 1638-1646
• ANDERSON, D., SELF, T., MELLOR, I.R., GOH, G., HILL, S.J. and DENNING, C., 2007. Transgenic enrichment of cardiomyocytes from human embryonic stem cells. Molecular Therapy. 15(11), 2027-2036
• DENNING, C., ALLEGRUCCI, C., PRIDDLE, H., BARBADILLO-MUÑOZ, M.D., ANDERSON, D., SELF, T., SMITH, N.M., PARKIN, C.T. and YOUNG, L.E., 2006. Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES-7. International Journal of Developmental Biology. 50(1), 27-37
• SELF, T.J., OAKLEY, S.M. and HILL, S.J., 2005. Clathrin-independent internalization of the human histamine H1-receptor in CHO-K1 cells British Journal of Pharmacology. 146(4), 612-24
• GOH, G., SELF, T., BARBADILLO MUÑOZ, M.D., HALL, I.P., YOUNG, L. and DENNING, C., 2005. Molecular and phenotypic analyses of human embryonic stem cell-derived cardiomyocytes: opportunities and challenges for clinical translation Thrombosis and Haemostasis. 94(4), 728-737
• QAZI, S.N.A., HARRISON, S.E., SELF, T., WILLIAMS, P. and HILL, P.J., 2004. Real-time monitoring of intracellular Staphylococcus aureus replication Journal of Bacteriology. 186(4), 1065-1077
• KROS, C J, MARCOTTI, W, VAN NETTEN, S M, SELF, T J, LIBBY, R T, BROWN, S D M, RICHARDSON, G P and STEEL, K P, 2002. Reduced climbing and increased slipping adaptation in cochlear hair cells of mice with Myo7a mutations. Nature Neuroscience. 5(1), 41-7
• MAKANYA, A N, SELF, T J, WARUI, C N and MWANGI, D K, 2001. Gut morphology and morphometry in the epauletted Wahlberg's fruit bat (Epomophorus wahlbergi, Sundevall, 1846). Acta Biologica Hungarica. 52(1), 75-89
• SELF, T, SOBE, T, COPELAND, N G, JENKINS, N A, AVRAHAM, K B and STEEL, K P, 1999. Role of myosin VI in the differentiation of cochlear hair cells. Developmental Biology. 214(2), 331-41
• SELF, T, MAHONY, M, FLEMING, J, WALSH, J, BROWN, S D and STEEL, K P, 1998. Shaker-1 mutations reveal roles for myosin VIIA in both development and function of cochlear hair cells. Development (Cambridge). 125(4), 557-66
• STEEL, K P, MBURU, P, GIBSON, F, WALSH, J, VARELA, A, BROWN, K, SELF, T, MAHONY, M, FLEMING, J, PEARCE, A, HARVEY, D, CABLE, J and BROWN, S D, 1997. Unravelling the genetics of deafness. Annals of Otology, Rhinology and Laryngology (Supplement). 168, 59-62
• MAYHEW, T M, MWAMENGELE, G L, SELF, T J and TRAVERS, J P, 1994. Stereological studies on red corpuscle size produce values different from those obtained using haematocrit- and model-based methods. British Journal of Haematology. 86(2), 355-60
• LASZLO, L, LOWE, J, SELF, T, KENWARD, N, LANDON, M, MCBRIDE, T, FARQUHAR, C, MCCONNELL, I, BROWN, J and HOPE, J, 1992. Lysosomes as key organelles in the pathogenesis of prion encephalopathies. Journal of Pathology. 166(4), 333-41
• LáSZLó, L, DOHERTY, F J, WATSON, A, SELF, T, LANDON, M, LOWE, J and MAYER, R J, 1991. Immunogold localisation of ubiquitin-protein conjugates in primary (azurophilic) granules of polymorphonuclear neutrophils. FEBS Letters. 279(2), 175-8
• LáSZLó, L, TUCKWELL, J, SELF, T, LOWE, J, LANDON, M, SMITH, S, HAWTHORNE, J N and MAYER, R J, 1991. The latent membrane protein-1 in Epstein-Barr virus-transformed lymphoblastoid cells is found with ubiquitin-protein conjugates and heat-shock protein 70 in lysosomes oriented around the microtubule organizing centre. Journal of Pathology. 164(3), 203-14
• WALLER, D A, THOMAS, N W and SELF, T J, 1988. Epithelial restitution in the large intestine of the rat following insult with bile salts. Virchows Archiv. A: Pathological anatomy and histopathology. 414(1), 77-81