School of Life Sciences
 

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Sophie Rochette

Research Technician, Faculty of Medicine & Health Sciences

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Biography

Having completed a Degree in Biochemistry, a Master in Environmental sciences, Chemistry and Health, and a PhD in Chemistry, I left the science for a couple of years and focused on its management. However my nurturing spirit brought me back into Research and Teaching by taking a research technical position at the School of Life Sciences at the Queen Medical Centre in Nottingham.

Expertise Summary

I am working at understanding the organization, regulation and function of genes. My technical expertise is based around Molecular Biology/Biochemistry. At the gene level, I can perform sub-cloning, site-directed mutagenesis, genotyping, gene expression. At the transcript level, utilizing cultured cells, I have recently trained to run and analyze qPCR experiments using common software packages. At the protein level, I am able to achieve protein/antibody purification, cell fractionation, immunoblotting, immunoprecipitation, immunocytochemistry, whole in situ hybridisation, bench flow cytometry. I am responsible for ensuring the running and safety of two busy teams, which includes teaching undergraduate and post-graduate students the basics of experimenting in a laboratory.

Teaching Summary

- Teaching of practical courses at the School of Pharmacy, University of Nottingham.

- Demonstration in the laboratory for UG/Msci/MedSci/PhD students.

- Participated to the Associate Teaching Program (ATP), awarded a Associate Fellow HEA.

Research Summary

Cancer and Heart Development.

Past Research

- School of Biosciences, Birmingham, England

Studies of cellular events after an incompatible response in the poppy pollen.

- Bayer Cropscience, Lyon, France

Development of a molecular tool, based on gene expression inhibition by RNA interference, to detect essential genes in the pathogenic rice fungus Magnaporthe grisea.

- INSERM, Marseille, France

Analysing gene expression profile by microarray in normal human melanocytes.

- Centre for Biomolecular Science, Nottingham, England

Structural studies of an enzyme with antibiotic properties.

- Grupo de investigación en Gerontología, A Coruña, Spain

Investigation of the factors associated to cognitive deterioration due to ageing so that to define the needs and improve the welfare resources of the elder ones.

- Queen Medical Center, Biomedical Sciences, Nottingham, England.

Working for Dr Sally Wheatley and Dr Siobhan Loughna

School of Life Sciences

University of Nottingham
Medical School
Queen's Medical Centre
Nottingham NG7 2UH

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