Contact

• workRoom B210 Biodiscovery Institute
  University Park
  Nottingham
  NG7 2RD
  UK
• Nick.Hannan@nottingham.ac.uk

Teaching Summary

Module convener (ONCG4013) Module 3 - Pluripotent Stem Cells (Semester 1)

Module convener (ONCG4015) Module 5 - Research Skills and Technology Exploitation (Semester 1 & 2)

Research Summary

My research interests are in mechanisms of endoderm development and disease. To study this we are using human stem cells to generate lung, liver, pancreatic and intestinal cells. We then use these… read more

Selected Publications

• SAMPAZIOTIS F, CARDOSO DE BRITO M, MADRIGAL P, BERTERO A, SAEB-PARSY K, SOARES FA, SCHRUMPF E, MELUM E, KARLSEN TH, BRADLEY JA, GELSON WT, DAVIES S, BAKER A, KASER A, ALEXANDER GJ, HANNAN NR and VALLIER L, 2015. Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nature biotechnology. 33(8), 845-52
• HANNAN NRF, SEGERITZ C, TOUBOUL T and VALLIER L, 2013. Production of hepatocyte-like cells from human pluripotent stem cells. Nature protocols. 8(2), 430-7
• HANNAN NRF, FORDHAM RP, SYED YA, MOIGNARD V, BERRY A, BAUTISTA R, HANLEY NA, JENSEN KB and VALLIER L, 2013. Generation of multipotent foregut stem cells from human pluripotent stem cells. Stem cell reports. 1(4), 293-306
• HANNAN NRF, SAMPAZIOTIS F, SEGERITZ C, HANLEY NA and VALLIER L, 2015. Generation of Distal Airway Epithelium from Multipotent Human Foregut Stem Cells. Stem cells and development. 24(14), 1680-90

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Current Research

My research interests are in mechanisms of endoderm development and disease. To study this we are using human stem cells to generate lung, liver, pancreatic and intestinal cells. We then use these cells to model early human development and understand mechanisms of cell fate choice. In addition to developmental studies we are also using these cells for disease modelling. We have also developed 3D culture platforms for the culture of primary human cells from the liver and intestines. Using this technology combined with our stem cell platforms we are modelling (with help from collaborators) several major diseases that affect endodermal organs including Crohn's disease, ulcerative colitis, idiopathic pulmonary fibrosis, cystic fibrosis, hepatitis B infection, drug induced liver injury and non-alcoholic fatty liver disease.

• RIMLAND, CASEY A, TILSON, SAMANTHA G, MORELL, CAROLA M, TOMAZ, RUTE A, LU, WEI-YU, ADAMS, SIMONE E, GEORGAKOPOULOS, NIKITAS, OTAIZO-CARRASQUERO, FRANCISCO, MYERS, TIMOTHY G, FERDINAND, JOHN R, GIESECK, RICHARD L 3RD, SAMPAZIOTIS, FOTIOS, TYSOE, OLIVIA C, WESLEY, BRANDON, MURARO, DANIELE, ONISCU, GABRIEL C, HANNAN, NICHOLAS RF, FORBES, STUART J, SAEB-PARSY, KOUROSH, WYNN, THOMAS A and VALLIER, LUDOVIC, 2020. Regional differences in human biliary tissues and corresponding in vitro derived organoids. Hepatology (Baltimore, Md.).
• DALLEYWATER, W. J., HANNAN, N., ROSE, F., WILDMAN, R. and ILYAS, M., 2019. A Novel 3D Cell Culture System Demonstrates Induced Pluripotent Stem Cells Driven to Intestinal Differentiation are Capable of Spontaneous Crypt Assembly JOURNAL OF PATHOLOGY. 249, S41-S41
• FORBESTER, JESSICA L, HANNAN, NICHOLAS, VALLIER, LUDOVIC and DOUGAN, GORDON, 2019. Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System. Methods in molecular biology (Clifton, N.J.). 1576, 157-169
• CHARIS-PATRICIA SEGERITZ, SHEIKH TAMIR RASHID, MIGUEL CARDOSO DE BRITO, MARIA PAOLA SERRA, ADRIANA ORDONEZ, CAROLA MARIA MORELL, JOSEPH E. KASERMAN, PEDRO MADRIGAL, NICHOLAS R.F. HANNAN, LAURENT GATTO, LU TAN, ANDREW A. WILSON, KATHRYN LILLEY, STEFAN J. MARCINIAK, BIBEK GOOPTU, DAVID A. LOMAS and LUDOVIC VALLIER, 2018. hiPSC hepatocyte model demonstrates the role of unfolded protein response and inflammatory networks in α1-antitrypsin deficiency Journal of Hepatology.
• DALLEYWATER, W. J., RAPOSO, T. PEREIRA, ROSE, F., HANNAN, N. and ILYAS, M., 2018. An Improved Monolayer Culture Technique for Driving Mouse Embryonic Stem Cells to Intestinal Differentiation JOURNAL OF PATHOLOGY. 246, S39-S39
• DALLEYWATER, W. J., RAPOSO, T. PEREIRA, ROSE, F., HANNAN, N. and ILYAS, M., 2018. A New Forced Aggregation Method for Generating Embryoid Bodies from Mouse Embryonic Stem Cells JOURNAL OF PATHOLOGY. 246, S40-S40
• FAIR, KATHRYN L., COLQUHOUN, JENNIFER and HANNAN, NICHOLAS R. F., 2018. Intestinal organoids for modelling intestinal development and disease PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES. 373(1750),
• LEWIS LC, LO PC, FOSTER JM, DAI N, CORRÊA IR, DURCZAK PM, DUNCAN G, RAMSAWHOOK A, AITHAL GP, DENNING C, HANNAN NR and RUZOV A, 2017. Dynamics of 5-carboxylcytosine during hepatic differentiation: Potential general role for active demethylation by DNA repair in lineage specification. Epigenetics. 12(4), 277-286
• SAMPAZIOTIS F, DE BRITO MC, GETI I, BERTERO A, HANNAN NR and VALLIER L, 2017. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells. Nature protocols. 12(4), 814-827
• SAMPAZIOTIS F, JUSTIN AW, TYSOE OC, SAWIAK S, GODFREY EM, UPPONI SS, GIESECK RL, DE BRITO MC, BERNTSEN NL, GÓMEZ-VÁZQUEZ MJ, ORTMANN D, YIANGOU L, ROSS A, BARGEHR J, BERTERO A, ZONNEVELD MCF, PEDERSEN MT, PAWLOWSKI M, VALESTRAND L, MADRIGAL P, GEORGAKOPOULOS N, PIRMADJID N, SKELDON GM, CASEY J, SHU W, MATEREK PM, SNIJDERS KE, BROWN SE, RIMLAND CA, SIMONIC I, DAVIES SE, JENSEN KB, ZILBAUER M, GELSON WTH, ALEXANDER GJ, SINHA S, HANNAN NRF, WYNN TA, KARLSEN TH, MELUM E, MARKAKI AE, SAEB-PARSY K and VALLIER L, 2017. Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids. Nature medicine.
• BERTERO A, PAWLOWSKI M, ORTMANN D, SNIJDERS K, YIANGOU L, CARDOSO DE BRITO M, BROWN S, BERNARD WG, COOPER JD, GIACOMELLI E, GAMBARDELLA L, HANNAN NR, IYER D, SAMPAZIOTIS F, SERRANO F, ZONNEVELD MC, SINHA S, KOTTER M and VALLIER L, 2016. Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs. Development (Cambridge, England). 143(23), 4405-4418
• FORBESTER JL, HANNAN N, VALLIER L and DOUGAN G, 2016. Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System. Methods in molecular biology (Clifton, N.J.). (In Press.)
• FORBESTER JL, GOULDING D, VALLIER L, HANNAN N, HALE C, PICKARD D, MUKHOPADHYAY S and DOUGAN G, 2015. Interaction of Salmonella enterica Serovar Typhimurium with Intestinal Organoids Derived from Human Induced Pluripotent Stem Cells. Infection and immunity. 83(7), 2926-34
• HANNAN NRF, SAMPAZIOTIS F, SEGERITZ C, HANLEY NA and VALLIER L, 2015. Generation of Distal Airway Epithelium from Multipotent Human Foregut Stem Cells. Stem cells and development. 24(14), 1680-90
• GIESECK RL, COLQUHOUN J and HANNAN NRF, 2015. Disease modeling using human induced pluripotent stem cells: lessons from the liver. Biochimica et biophysica acta. 1851(1), 76-89
• SAMPAZIOTIS F, CARDOSO DE BRITO M, MADRIGAL P, BERTERO A, SAEB-PARSY K, SOARES FA, SCHRUMPF E, MELUM E, KARLSEN TH, BRADLEY JA, GELSON WT, DAVIES S, BAKER A, KASER A, ALEXANDER GJ, HANNAN NR and VALLIER L, 2015. Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nature biotechnology. 33(8), 845-52
• GIESECK RL, VALLIER L and HANNAN NR, 2015. Generation of Hepatocytes from Pluripotent Stem Cells for Drug Screening and Developmental Modeling. Methods in molecular biology (Clifton, N.J.). 1250, 123-42
• GIESECK RL, HANNAN NRF, BORT R, HANLEY NA, DRAKE RAL, CAMERON GWW, WYNN TA and VALLIER L, 2014. Maturation of induced pluripotent stem cell derived hepatocytes by 3D-culture. PloS one. 9(1), e86372
• HANNAN NRF, FORDHAM RP, SYED YA, MOIGNARD V, BERRY A, BAUTISTA R, HANLEY NA, JENSEN KB and VALLIER L, 2013. Generation of multipotent foregut stem cells from human pluripotent stem cells. Stem cell reports. 1(4), 293-306
• FORDHAM RP, YUI S, HANNAN NRF, SOENDERGAARD C, MADGWICK A, SCHWEIGER PJ, NIELSEN OH, VALLIER L, PEDERSEN RA, NAKAMURA T, WATANABE M and JENSEN KB, 2013. Transplantation of expanded fetal intestinal progenitors contributes to colon regeneration after injury. Cell stem cell. 13(6), 734-44
• HANNAN NRF, SEGERITZ C, TOUBOUL T and VALLIER L, 2013. Production of hepatocyte-like cells from human pluripotent stem cells. Nature protocols. 8(2), 430-7
• CHO CH, HANNAN NR, DOCHERTY FM, DOCHERTY HM, JOÅO LIMA M, TROTTER MWB, DOCHERTY K and VALLIER L, 2012. Inhibition of activin/nodal signalling is necessary for pancreatic differentiation of human pluripotent stem cells. Diabetologia. 55(12), 3284-95
• YUSA K, RASHID ST, STRICK-MARCHAND H, VARELA I, LIU P, PASCHON DE, MIRANDA E, ORDÓÑEZ A, HANNAN NRF, ROUHANI FJ, DARCHE S, ALEXANDER G, MARCINIAK SJ, FUSAKI N, HASEGAWA M, HOLMES MC, DI SANTO JP, LOMAS DA, BRADLEY A and VALLIER L, 2011. Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells. Nature. 478(7369), 391-4
• BROWN S, TEO A, PAUKLIN S, HANNAN N, CHO CH, LIM B, VARDY L, DUNN NR, TROTTER M, PEDERSEN R and VALLIER L, 2011. Activin/Nodal signaling controls divergent transcriptional networks in human embryonic stem cells and in endoderm progenitors. Stem cells (Dayton, Ohio). 29(8), 1176-85
• RASHID ST, CORBINEAU S, HANNAN N, MARCINIAK SJ, MIRANDA E, ALEXANDER G, HUANG-DORAN I, GRIFFIN J, AHRLUND-RICHTER L, SKEPPER J, SEMPLE R, WEBER A, LOMAS DA and VALLIER L, 2010. Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells. The Journal of clinical investigation. 120(9), 3127-36
• TOUBOUL T, HANNAN NRF, CORBINEAU S, MARTINEZ A, MARTINET C, BRANCHEREAU S, MAINOT S, STRICK-MARCHAND H, PEDERSEN R, DI SANTO J, WEBER A and VALLIER L, 2010. Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development. Hepatology (Baltimore, Md.). 51(5), 1754-65
• HANNAN NRF, JAMSHIDI P, PERA MF and WOLVETANG EJ, 2009. BMP-11 and myostatin support undifferentiated growth of human embryonic stem cells in feeder-free cultures. Cloning and stem cells. 11(3), 427-35
• VALLIER L, TOUBOUL T, CHNG Z, BRIMPARI M, HANNAN N, MILLAN E, SMITHERS LE, TROTTER M, RUGG-GUNN P, WEBER A and PEDERSEN RA, 2009. Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. PloS one. 4(6), e6082
• HANNAN NRF and WOLVETANG EJ, 2009. Adipocyte differentiation in human embryonic stem cells transduced with Oct4 shRNA lentivirus. Stem cells and development. 18(4), 653-60
• GAO Y, HANNAN NRF, WANYONYI S, KONSTANTOPOLOUS N, PAGNON J, FENG HC, JOWETT JBM, KIM K, WALDER K and COLLIER GR, 2006. Activation of the selenoprotein SEPS1 gene expression by pro-inflammatory cytokines in HepG2 cells. Cytokine. 33(5), 246-51