Ingrid Dreveny

Contact

• workRoom C97 Biodiscovery Institute
  University Park
  Nottingham
  NG7 2RD
  UK
• work0115 8468015
• ingrid.dreveny@nottingham.ac.uk

Biography

I received my training as a chemist at the University of Graz. As a Masters student at the University Louis Pasteur, Strasbourg, I studied the folding and structure of synthetic peptides. This work sparked my interest and fascination for protein structure from which I pursued a Ph.D. in protein crystallography under the supervision of Christoph Kratky, working on the structure and mechanism of an unusual flavoprotein that has wide-ranging applications in industrial biocatalysis.

During my postdoctoral years at Imperial College London in the laboratory of Paul Freemont and Xiaodong Zhang I uncovered how the multi-functional molecular machine, the AAA ATPase p97, that has been associated with neurodegenerative diseases and cancer, recognizes adaptor proteins. After a brief period as research fellow in the School of Pharmacy, University of Nottingham, I was appointed lecturer in protein crystallography.

Research Summary

I am interested in how macromolecular structure determines function and can help explain cellular events. My main area of expertise is macromolecular X-ray crystallography, which I combine with other… read more

Recent Publications

• SANCHEZ-BAILON, MARIA PILAR, CHOI, SOO-YOUN, DUFFICY, ELIZABETH R., SHARMA, KARAN, MCNEE, GAVIN S., GUNNELL, EMMA, CHIANG, KELLY, SAHAY, DEBASHISH, MASLEN, SARAH, STEWART, GRANT S., SKEHEL, J. MARK, DREVENY, INGRID and DAVIES, CLARE C., 2021. Arginine methylation and ubiquitylation crosstalk controls DNA end-resection and homologous recombination repair NATURE COMMUNICATIONS. 12(1),
• OLIVA, MARIA LUIZA VILELA, DREVENY, INGRID and EMSLEY, JONAS, 2020. Exosites expedite blood coagulation JOURNAL OF BIOLOGICAL CHEMISTRY. 295(45), 15208-15209
• KAIRA, BUBACARR G., SLATER, ALEXANDRE, MCCRAE, KEITH R., DREVENY, INGRID, SUMYA, UMMAY, MUTCH, NICOLA J., SEARLE, MARK and EMSLEY, JONAS, 2020. Factor XII and kininogen asymmetric assembly with gC1qR/C1QBP/P32 is governed by allostery BLOOD. 136(14), 1685-1697
• GUNNELL, EMMA A., AL-NOORI, ALAA, MUHSEN, USAMA, DAVIES, CLARE C., DOWDEN, JAMES and DREVENY, INGRID, 2020. Structural and biochemical evaluation of bisubstrate inhibitors of protein arginine N-methyltransferases PRMT1 and CARM1 (PRMT4) BIOCHEMICAL JOURNAL. 477(4), 787-800

• View all publications

Current Research

I am interested in how macromolecular structure determines function and can help explain cellular events. My main area of expertise is macromolecular X-ray crystallography, which I combine with other biophysical and biochemical techniques. My research is aimed at:

• Establishing protein structure - function relationships
• Characterising protein-protein and protein-ligand interactions
• Uncovering the molecular basis of human diseases

Current research topics include:

• Structure and function of proteases in the ubiquitin pathway
• Molecular mechanism of enzymes in posttranslational modification pathways
• Protein complexes in ciliary trafficking
• Enzymes for pharmaceutical applications

For further details please visit the Structural Biology group website.

Memberships of Committees & Professional Bodies

Biochemical Society; British Crystallographic Association

• SANCHEZ-BAILON, MARIA PILAR, CHOI, SOO-YOUN, DUFFICY, ELIZABETH R., SHARMA, KARAN, MCNEE, GAVIN S., GUNNELL, EMMA, CHIANG, KELLY, SAHAY, DEBASHISH, MASLEN, SARAH, STEWART, GRANT S., SKEHEL, J. MARK, DREVENY, INGRID and DAVIES, CLARE C., 2021. Arginine methylation and ubiquitylation crosstalk controls DNA end-resection and homologous recombination repair NATURE COMMUNICATIONS. 12(1),
• OLIVA, MARIA LUIZA VILELA, DREVENY, INGRID and EMSLEY, JONAS, 2020. Exosites expedite blood coagulation JOURNAL OF BIOLOGICAL CHEMISTRY. 295(45), 15208-15209
• KAIRA, BUBACARR G., SLATER, ALEXANDRE, MCCRAE, KEITH R., DREVENY, INGRID, SUMYA, UMMAY, MUTCH, NICOLA J., SEARLE, MARK and EMSLEY, JONAS, 2020. Factor XII and kininogen asymmetric assembly with gC1qR/C1QBP/P32 is governed by allostery BLOOD. 136(14), 1685-1697
• GUNNELL, EMMA A., AL-NOORI, ALAA, MUHSEN, USAMA, DAVIES, CLARE C., DOWDEN, JAMES and DREVENY, INGRID, 2020. Structural and biochemical evaluation of bisubstrate inhibitors of protein arginine N-methyltransferases PRMT1 and CARM1 (PRMT4) BIOCHEMICAL JOURNAL. 477(4), 787-800
• LI C, VOOS KM, PATHAK M, HALL G, MCCRAE KR, DREVENY I, LI R and EMSLEY J, 2019. Plasma kallikrein structure reveals apple domain disc rotated conformation compared to factor XI. Journal of thrombosis and haemostasis : JTH. (In Press.)
• PADROSA, DAVID ROURA, ALAUX, RAPHAEL, SMITH, PHILLIP, DREVENY, INGRID, LOPEZ-GALLEGO, FERNANDO and PARADISI, FRANCESCA, 2019. Enhancing PLP-Binding Capacity of Class-III omega-Transaminase by Single Residue Substitution FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY. 7,
• PATHAK, MONIKA, MANNA, ROSA, LI, CHAN, KAIRA, BUBACARR G., HAMAD, BADRALDIN KAREEM, BELVISO, BENNY DANILO, BONTURI, CAMILA R., DREVENY, INGRID, FISCHER, PETER M., DEKKER, LODEWIJK V., VILELA OLIVA, MARIA LUIZA and EMSLEY, JONAS, 2019. Crystal structures of the recombinant -factor XIIa protease with bound Thr-Arg and Pro-Arg substrate mimetics ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY. 75, 578-591
• RIEGLER-BERKET L, LEITMEIER A, ASCHAUER P, DREVENY I and OBERER M, 2018. Identification of lipases with activity towards monoacylglycerol by criterion of conserved cap architectures. Biochimica et biophysica acta. 1863(7), 679-687
• WARD SJ, GRATTON HE, INDRAYUDHA P, MICHAVILA C, MUKHOPADHYAY R, MAURER SK, CAULTON SG, EMSLEY J and DREVENY I, 2018. The structure of the deubiquitinase USP15 reveals a misaligned catalytic triad and an open ubiquitin-binding channel. The Journal of biological chemistry. 293(45), 17362-17374
• SPILIOTOPOULOS A, BLOKPOEL FERRERAS L, DENSHAM RM, CAULTON SG, MADDISON BC, MORRIS JR, DIXON JE, GOUGH KC and DREVENY I, 2018. Discovery of peptide ligands targeting a specific ubiquitin-like domain-binding site in the deubiquitinase USP11. The Journal of biological chemistry. 294(2), 424-436
• DREES SL, LI C, PRASETYA F, SALEEM M, DREVENY I, WILLIAMS P, HENNECKE U, EMSLEY J and FETZNER S, 2016. PqsBC, a Condensing Enzyme in the Biosynthesis of the Pseudomonas aeruginosa Quinolone Signal: CRYSTAL STRUCTURE, INHIBITION, AND REACTION MECHANISM. J Biol Chem. 291(13), 6610-24
• PATHAK M, WILMANN P, AWFORD J, LI C, HAMAD BK, FISCHER PM, DREVENY I, DEKKER LV and EMSLEY J, 2015. Coagulation factor XII protease domain crystal structure. Journal of Thrombosis and Haemostasis. 13(4), 580-591
• SPILIOTOPOULOS A, OWEN JP, MADDISON BC, DREVENY I, REES HC and GOUGH KC, 2015. Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets. Journal of immunological methods. 420, 50-5
• DREVENY, I., DEEVES, S.E., FULTON, J., YUE, B., MESSMER, M., BHATTACHARYA, A., COLLINS, H.M. and HEERY, D.M., 2014. The double PHD finger domain of MOZ/MYST3 induces α-helical structure of the histone H3 tail to facilitate acetylation and methylation sampling and modification Nucleic Acids Research. 42(2), 822-835
• DEMPSTER S, HARPER S, MOSES JE and DREVENY I, 2014. Structural characterization of the apo form and NADH binary complex of human lactate dehydrogenase. Acta crystallographica. Section D, Biological crystallography. 70(Pt 5), 1484-90
• HARPER S, GRATTON HE, CORNACIU I, OBERER M, SCOTT DJ, EMSLEY J and DREVENY I, 2014. Structure and Catalytic Regulatory Function of Ubiquitin Specific Protease 11 N-Terminal and Ubiquitin-like Domains. Biochemistry. 53(18), 2966-78
• PATHAK, M., WONG, S.S., DREVENY, I. and EMSLEY, J., 2013. Structure of plasma and tissue kallikreins Thrombosis and Haemostasis. 110(3), 423-433
• RENGACHARI S, ASCHAUER P, SCHITTMAYER M, MAYER N, GRUBER K, BREINBAUER R, BIRNER-GRUENBERGER R, DREVENY I and OBERER M, 2013. Conformational plasticity and ligand binding of bacterial monoacylglycerol lipase. The Journal of Biological Chemistry. 288(43), 31093-104
• RENGACHARI, S., BEZERRA, G.A., RIEGLER-BERKET, L., GRUBER, C.C., STURM, C., TASCHLER, U., BOESZOERMENYI, A., DREVENY, I., ZIMMERMANN, R., GRUBER, K. and OBERER, M., 2012. The structure of monoacylglycerol lipase from Bacillus sp. H257 reveals unexpected conservation of the cap architecture between bacterial and human enzymes Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids. 1821(7), 1012-1021
• HARPER, S., BESONG, T.M.D., EMSLEY, J., SCOTT, D.J. and DREVENY, I., 2011. Structure of the USP15 N-terminal domains: a β-hairpin mediates close association between the DUSP and UBL domains Biochemistry. 50(37), 7995-8004
• DREVENY, I., ANDRYUSHKOVA, A.S., GLIEDER, A., GRUBER, K. and KRATKY, C., 2009. Substrate binding in the FAD-dependent hydroxynitrile lyase from almond provides insight into the mechanism of cyanohydrin formation and explains the absence of dehydrogenation activity Biochemistry. 48(15), 3370-3377
• BEURON, FABIENNE, DREVENY, INGRID, YUAN, XUEMEI, PYE, VALERIE E, MCKEOWN, CIARAN, BRIGGS, LOUISE C, CLIFF, MATTHEW J, KANEKO, YAYOI, WALLIS, RUSSELL, ISAACSON, RIVKA L, LADBURY, JOHN E and MATTHEWS, STEV, 2006. Conformational changes in the AAA ATPase p97-p47 adaptor complex. EMBO Journal. 25(9), 1967-76
• UCHIYAMA, KEIJI, TOTSUKAWA, GO, PUHKA, MAIJA, KANEKO, YAYOI, JOKITALO, EIJA, DREVENY, INGRID, BEURON, FABIENNE, ZHANG, XIAODONG, FREEMONT, PAUL and KONDO, HISAO, 2006. p37 is a p97 adaptor required for Golgi and ER biogenesis in interphase and at the end of mitosis. Developmental Cell. 11(6), 803-16
• PYE, VALERIE E, DREVENY, INGRID, BRIGGS, LOUISE C, SANDS, CAROLINE, BEURON, FABIENNE, ZHANG, XIAODONG and FREEMONT, PAUL S, 2006. Going through the motions: the ATPase cycle of p97. Journal of Structural Biology. 156(1), 12-28
• DREVENY, INGRID, KONDO, HISAO, UCHIYAMA, KEIJI, SHAW, ANTHONY, ZHANG, XIAODONG and FREEMONT, PAUL S, 2004. Structural basis of the interaction between the AAA ATPase p97/VCP and its adaptor protein p47. EMBO Journal. 23(5), 1030-9
• YUAN, XUEMEI, SIMPSON, PETER, KONDO, HISAO, MCKEOWN, CIARAN, DREVENY, INGRID, ZHANG, XIAODONG, FREEMONT, PAUL S and MATTHEWS, STEPHEN, 2004. Complete backbone resonance assignments of p47: the 41kDa adaptor protein of the AAA ATPase p97. Journal of Biomolecular NMR. 28(3), 309-10
• DREVENY, I, PYE, V E, BEURON, F, BRIGGS, L C, ISAACSON, R L, MATTHEWS, S J, MCKEOWN, C, YUAN, X, ZHANG, X and FREEMONT, P S, 2004. p97 and close encounters of every kind: a brief review. Biochemical Society Transactions. 32(Pt 5), 715-20
• YUAN, XUEMEI, SIMPSON, PETER, MCKEOWN, CIARAN, KONDO, HISAO, UCHIYAMA, KEIJI, WALLIS, RUSSELL, DREVENY, INGRID, KEETCH, CATHERINE, ZHANG, XIAODONG, ROBINSON, CAROL, FREEMONT, PAUL and MATTHEWS, STEPHEN, 2004. Structure, dynamics and interactions of p47, a major adaptor of the AAA ATPase, p97. EMBO Journal. 23(7), 1463-73
• GLIEDER, ANTON, WEIS, ROLAND, SKRANC, WOLFGANG, POECHLAUER, PETER, DREVENY, INGRID, MAJER, SANDRA, WUBBOLTS, MARCEL, SCHWAB, HELMUT and GRUBER, KARL, 2003. Comprehensive step-by-step engineering of an (R)-hydroxynitrile lyase for large-scale asymmetric synthesis. Angewandte Chemie International Edition. 42(39), 4815-8
• DREVENY, INGRID, KRATKY, CHRISTOPH and GRUBER, KARL, 2002. The active site of hydroxynitrile lyase from Prunus amygdalus: modeling studies provide new insights into the mechanism of cyanogenesis. Protein Science. 11(2), 292-300
• DREVENY, I, GRUBER, K, GLIEDER, A, THOMPSON, A and KRATKY, C, 2001. The hydroxynitrile lyase from almond: a lyase that looks like an oxidoreductase. Structure. 9(9), 803-15