Grantley Lycett

Contact

• workPlant Sciences
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• Grantley.Lycett1@nottingham.ac.uk
• http://www.nottingham.ac.uk/biosciences/people/grantley.lycett1

Research Summary

The main focus of my recent research has been on the Rab GTPases, which control trafficking from the trans-Golgi network to the cell membrane. We have shown that manipulation of these can cause… read more

Recent Publications

• LAWSON, T., LYCETT, G.W., MAYES, S., HO, W.K. and CHIN, C.F., 2020. Transcriptome-wide identification and characterization of the Rab GTPase family in mango. Molecular Biology Reports. 47(6), 4183-4197
• LAWSON, T., LYCETT, G.W., ALI, A. and CHIN, C.F., 2019. Characterization of Southeast Asian mangoes (Mangifera indica L.) according to their physicochemical attributes. Scientia Horticulturae. 243, 189-196
• CHEE, M.J.Y., LYCETT, G.W. and CHIN, C.F., 2018. Development of a direct transformation method by GFP screening and in vitro whole plant regeneration of Capsicum frutescens L. Electronic Journal of Biotechnology. 34, 51-58
• LAWSON, T., MAYES, S., LYCETT, G.W. and CHIN, C.F., 2018. Plant Rabs and the role in fruit ripening Biotechnology and Genetic Engineering Reviews. 34(2), 181-197

• View all publications

Current Research

The main focus of my recent research has been on the Rab GTPases, which control trafficking from the trans-Golgi network to the cell membrane. We have shown that manipulation of these can cause changes in the composition of the cell wall and in important processes such as shelf life of fruit and the ability to break down cell wall material for potential use as biofuel.

In addition, I have worked on other biotechnological problems such as vanilla production in transgenic plants, ethylene production in plants and seed storage proteins.

• LAWSON, T., LYCETT, G.W., MAYES, S., HO, W.K. and CHIN, C.F., 2020. Transcriptome-wide identification and characterization of the Rab GTPase family in mango. Molecular Biology Reports. 47(6), 4183-4197
• LAWSON, T., LYCETT, G.W., ALI, A. and CHIN, C.F., 2019. Characterization of Southeast Asian mangoes (Mangifera indica L.) according to their physicochemical attributes. Scientia Horticulturae. 243, 189-196
• CHEE, M.J.Y., LYCETT, G.W. and CHIN, C.F., 2018. Development of a direct transformation method by GFP screening and in vitro whole plant regeneration of Capsicum frutescens L. Electronic Journal of Biotechnology. 34, 51-58
• LAWSON, T., MAYES, S., LYCETT, G.W. and CHIN, C.F., 2018. Plant Rabs and the role in fruit ripening Biotechnology and Genetic Engineering Reviews. 34(2), 181-197
• CHEE, M.J.Y., LYCETT, G.W., KHOO, T-J. and CHIN, C.F., 2017. Bioengineering of the plant culture of Capsicum frutescens with vanillin synthase gene for the production of vanillin. Molecular Biotechnology. 59(1), 1-8
• LUNN, D., IBBETT, R., TUCKER, G.A. and LYCETT, G.W., 2015. Impact of altered cell wall composition on saccharification efficiency in stem tissue of Arabidopsis RABA GTPase deficient knockout mutants. Bioenergy Research. 8(3), 1362-1370
• TYLER, A.M., BHANDARI, D.G., POOLE, M., NAPIER, J.A., JONES, H.D., LU, C. and LYCETT, G.W., 2015. Gluten quality of bread wheat is associated with activity of RabD GTPases. Plant Biotechnology Journal. 13(2), 163-176
• LUNN, D., GADDIPATI, S.R., TUCKER, G.A. and LYCETT, G.W., 2013. Null mutants of individual RABA genes impact the proportion of different cell wall components in stem tissue of Arabidopsis thaliana. PLoS ONE. 8(10), e75724
• LUNN, D., PHAN, T.D., TUCKER, G.A. and LYCETT, G.W., 2013. Cell wall composition of tomato fruit changes during development and inhibition of vesicle trafficking is associated with reduced pectin levels and reduced softening Plant Physiology and Biochemistry. 66, 91-97
• GRIERSON, D., HAMILTON, A.J. and LYCETT, G.W., 2013. The life and times of ACC oxidase, alias TOM13 Molecular Biology Reports. 40(4), 3021-3022
• TYLER, A.M., POOLE, M., BHANDARI, D.G., NAPIER, J.A., JONES, H.D., LU, C. and LYCETT, G.W., 2011. Manipulating Rab GTPase activity in wheat to improve gluten quality for breadmaking. Aspects of Applied Biology. 110, 15-23
• REHMAN, R.U., STIGLIANO, E., LYCETT, G.W., STICHER, L., SBANO, F., FARACO, M., DALESSANDRO, G. and DI SANSEBASTIANO, G-P., 2008. Tomato Rab11a characterization evidenced a difference between Syp121-dependent and Syp122-dependent exocytosis Plant & Cell Physiology. 49(5), 751-766
• LYCETT, G., 2008. The role of Rab GTPases in cell wall metabolism Journal of Experimental Botany. 59(15), 4061-4074
• PHAN, T.D., BO, W., WEST, G., LYCETT, G.W. and TUCKER, GA, 2007. Silencing of the major salt-dependent isoform of pectinesterase in tomato alters fruit softening Plant Physiology. 144(4), 1960-1967
• ZAINAL, Z., ZAINAL, Z. and LYCETT, G.W., 2001. Construction of a mango (Mangifera indica L.) fruit cDNA library Journal of Tropical Agriculture and Food Science. 29, 53-59
• LU, C., ZAINAL, Z., TUCKER, G.A. and LYCETT, G.W., 2001. Developmental abnormalities and reduced fruit softening in tomato plants expressing an antisense Rab11 GTPase gene The Plant Cell. 13(8), 1819-1833
• LYCETT G.W., 2000. The identification of ethene biosynthetic genes by gene silencing. Methods in Molecular Biology. 141, 145-155
• JONES, C. G., SCOTHERN, G. P., LYCETT, G. W. and TUCKER, G. A., 1998. The effect of chimeric transgene architecture on co-ordinated gene silencing Planta. 204(4), 499-505
• LYCETT, G. W., ZAINAL, Z, LOS, M., FINDLAY, C. L. and TUCKER, G. A., 1997. Novel Ripening-Specific cDNA Clones from Mango Fruit Acta Horticulturae. 455(1), 277-286
• JONES, C. G., LYCETT, G. W. and TUCKER, G. A., 1996. Protease inhibitor studies and cloning of a serine carboxypeptidase cDNA from germinating seeds of pea (Pisum sativum L.) European Journal of Biochemistry. 235(3), 574-578
• ZAINAL, Z, TUCKER, G A and LYCETT, G W, 1996. A rab11-like gene is developmentally regulated in ripening mango (Mangifera indica L.) fruit. Biochimica et Biophysica Acta. 1314(3), 187-190
• BAILEY, P.C., LYCETT, G.W. and ROBERTS, J.A., 1996. A molecular study of dormancy-breaking and germination in seeds of Trollius ledebourii. Plant Molecular Biology. 32(3), 559-564
• GRIERSON, D., LYCETT, G.W. and TUCKER, G.A., eds., 1996. Mechanisms and applications of gene silencing. Nottingham University Press.
• JONES, C.G., SEYMOUR, G.B., BIRD, C.R., SCHUCH, W., BONIWELL, J., LYCETT, G.W. and TUCKER, G.A., 1996. Down regulation of two non-homologous endogenous genes with a single chimeric gene construct.. In: GRIERSON, D., LYCETT, G.W. and TUCKER, G.A., eds., Mechanisms and Applications of Gene Silencing Nottingham University Press. 85-89
• JONES, C.G., TUCKER, G.A. and LYCETT, G.W., 1996. Pattern of expression and characteristics of a cysteine proteinase cDNA from germinating seeds of pea (Pisum sativum L.) Biochimica et Biophysica Acta-Protein Structure and Molecular Enzymology. 1296(1), 13-15
• ENGLISH, P.J, LYCETT, G.W and ROBERTS, J.A. AND JACKSON, M.B., 1995. Increased 1-aminocyclopropane-1-carboxylic acid oxidase activity in shoots of flooded tomato plants raises ethylene production to physiologically active levels. Plant Physiology. 109, 1435-1440
• FRAY, R.G, WALLACE, A and GRIERSON, D. AND LYCETT, G.W., 1994. Nucleotide sequence and expression of a ripening and water stress-related cDNA from tomato with homology to the MIP class of membrane channel proteins. Plant Molecular Biology. 24, 539-543
• GRIERSON, D, HAMILTON, AJ, BOUZAYEN, M, KOCK, M, LYCETT, GW and BARTON, S, 1992. Inducible plant proteins: Regulation of gene expression, ethylene synthesis and ripening in transgenic tomatoes. In: S.E.B. Seminar Series, Cambridge University Press.. 49. 155-174
• HAMILTON, A.J., LYCETT, G.W. and GRIERSON, D., 1990. Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants. Nature. 346(6281), 284-287
• LYCETT, G W and PRITCHARD, R H, 1986. Functioning of the F-plasmid origin of replication in an Escherichia coli K12 Hfr strain during exponential growth. Plasmid. 16(3), 168-74
• EVANS, I.M, BOWN, D, LYCETT, G.W, CROY, R.R.D and BOULTER, D. AND GATEHOUSE, J.A., 1985. Transcription of a legumin gene from pea (Pisum sativum L.) in vitro. Planta. 165(4), 554-560
• LYCETT, G W, CROY, R R, SHIRSAT, A H, RICHARDS, D M and BOULTER, D, 1985. The 5'-flanking regions of three pea legumin genes: comparison of the DNA sequences. Nucleic Acids Research. 13(18), 6733-43
• LYCETT, G.W, DELAUNEY, A.J, ZHAO, W, GATEHOUSE, J.A, CROY, R.R.D. and BOULTER, D., 1984. Two cDNA clones coding for the legumin protein of Pisum sativum L. contain sequence repeats. Plant Molecular Biology. 3(2), 91-96
• EVANS, I.M, GATEHOUSE, J.A, LYCETT, G.W. and BOULTER, D., 1984. Use of synthetic oligodeoxyribonucleotides and primed cDNA as probes for pea (Pisum sativum L.) RNA and genomic DNA sequences. Plant Molecular Biology. 3(2), 73-81
• LYCETT, G W, CROY, R R, SHIRSAT, A H and BOULTER, D, 1984. The complete nucleotide sequence of a legumin gene from pea (Pisum sativum L.). Nucleic Acids Research. 12(11), 4493-506
• CROY, R.R.D., LYCETT, G.W., GATEHOUSE, J.A. and BOULTER, D., 1984. Synthesis, cloning and sequencing of pea (Pisum sativum L.) storage protein specific cDNAs. Kulturpflanze. 32, S81-S96
• GATEHOUSE, J A, LYCETT, G W, DELAUNEY, A J, CROY, R R and BOULTER, D, 1983. Sequence specificity of the post-translational proteolytic cleavage of vicilin, a seed storage protein of pea (Pisum sativum L.). Biochemical Journal. 212(2), 427-32
• LYCETT, G W, DELAUNEY, A J, GATEHOUSE, J A, GILROY, J, CROY, R R and BOULTER, D, 1983. The vicilin gene family of pea (Pisum sativum L.): a complete cDNA coding sequence for preprovicilin. Nucleic Acids Research. 11(8), 2367-80
• LYCETT, G.W and DELAUNEY, A.J. AND CROY R.R.D., 1983. Are plant genes different? FEBS Letters. 153(1), 43-46
• CROY, R.R.D, LYCETT, G.W, GATEHOUSE, J.A and YARWOOD, J.N. AND BOULTER, D., 1982. Cloning and analysis of cDNAs encoding plant storage protein precursors. Nature. 295(5844), 76-79
• GATEHOUSE, J A, LYCETT, G W, CROY, R R and BOULTER, D, 1982. The post-translational proteolysis of the subunits of vicilin from pea (Pisum sativum L.). Biochemical Journal. 207(3), 629-32
• LYCETT, G W, ORR, E and PRITCHARD, R H, 1980. Chloramphenicol releases a block in initiation of chromosome replication in a dnaA strain of Escherichia coli K12. Molecular and General Genetics. 178(2), 329-36