Klaus Winzer

Contact

• workRoom B12 BDI - Biodiscovery Institute
  University Park
  Nottingham
  NG7 2RD
  UK
• work0115 8232247
• klaus.winzer@nottingham.ac.uk

Biography

Diploma degree in Biology, Göttingen, Germany, 1992

PhD, Göttingen, 1995

Research Fellow, School of University of Nottingham, 1996

Senior Research Fellow, University of Nottingham, 2000

Lecturer, Department of Biological Sciences, University of Lancaster, 2006

Associate Professor, School of Life Sciences, University of Nottingham, 2008

Expertise Summary

Bacterial metabolism and physiology, Clostridia, CO-utilising bacteria, quorum-sensing, metabolic engineering, synthetic biology

Research Summary

Klaus Winzer is a microbiologist with more than 25 years' experience in bacterial physiology and metabolism. He is experienced in bacterial strain isolation, characterisation and engineering, and has… read more

Selected Publications

• ARENAS-LÓPEZ C, LOCKER J, OROL D, WALTER F, BUSCHE T, KALINOWSKI J, MINTON NP, KOVÁCS K and WINZER K, 2019. The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16. Biotechnology for Biofuels. 12, 150
• MAGENNIS E.P., FERNANDEZ-TRILLO F., SUI C., SPAIN S.S., CHURCHLEY D., MANTOVANI G, WINZER K. and ALEXANDER C., 2014. Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling. Nature Materials. 13, 748-755
• DARCH, S.E., WEST, S.A., WINZER, K. and DIGGLE, S.P., 2012. Density-dependent fitness benefits in quorum-sensing bacterial populations Proceedings of the National Academy of Sciences of the United States of America. 109(21), 8259-8263
• GARDNER, P.M., WINZER, K. and DAVIS, B.G., 2009. Sugar synthesis in a protocellular model leads to a cell signalling response in bacteria Nature Chemistry. 1(5), 377-383

• View all publications

Current Research

Klaus Winzer is a microbiologist with more than 25 years' experience in bacterial physiology and metabolism. He is experienced in bacterial strain isolation, characterisation and engineering, and has worked extensively in the field of bacterial cell-cell communication. Over his career, Klaus has studied a wide range of physiologically diverse bacteria including numerous pathogens and industrial strains, with an increasing focus on C1-utilising bacteria.

Current efforts focus on understanding and engineering the metabolism of anaerobic clostridia and aerobic gas-fermenting Cupriavidus species, with the ultimate aim of generating platform organisms for the production of biofuels and chemical commodities.

His group is investigating the molecular, genetic, and evolutionary mechanisms underlying bacterial strain degeneration and studying the roles of bacterial signalling in high density fermentations, with the goal of developing more stable and better performing production strains. In collaboration with computational scientists and mathematical modellers the team is working towards integrating complex metabolic pathways into new hosts, using a combination of synthetic biology approaches and in vitro evolution. Current examples include the engineering of carbon monoxide-tolerant and utilising strains for improved fermentation of industrial waste gases.

• HUMPHREYS JR, DEBEBE BJ, DIGGLE SP, WINZER K, 2023. Clostridium beijerinckii strain degeneration is driven by the loss of Spo0A activity Frontiers in Microbiology. 13, 1075609
• DUBERN J-F, HALLIDAY N, CÁMARA M, WINZER K, BARRETT DA, HARDIE KR, WILLIAMS P, 2023. Growth rate and nutrient limitation as key drivers of extracellular quorum sensing signal molecule accumulation in Pseudomonas aeruginosa. Microbiology. 169, 001316
• WICKHAM-SMITH C, MALYS N, WINZER K, 2023. Improving carbon monoxide tolerance of Cupriavidus necator H16 through adaptive laboratory evolution. Front. Bioeng. Biotechnol. 11, 1178536
• HUMPHREYS JR, BEAN Z, TWYCROSS J, WINZER K, 2023. The lanthipeptide synthetase-like protein CA_C0082 is an effector of Agr quorum sensing in Clostridium acetobutylicum. Microorganisms. 11(6), 1460
• DELAVELLE MP, BAKER JP, MILLARD J, WINZER K, MINTON NP, 2023. Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum. Front. Bioeng. Biotechnol. 11, 1213236
• PIATEK P, HUMPHREYS C, RAUT M, WRIGHT PC, SIMPSON S, KÖPKE M, MINTON NP, WINZER K, 2022. Agr quorum sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum. Scientific Reports. 12, 411
• WOODS C, HUMPHREYS CM,TOMI-ANDRINO C, HENSTRA AM, KÖPKE M, SIMPSON SD, WINZER K AND MINTON NP., 2022. Required gene set for autotrophic growth of Clostridium autoethanogenum. Applied and Environmental Microbiology. 88, e0247921
• PEARCY N, GARAVAGLIA M, MILLAT T, GILBERT JP, SONG Y, HARTMAN H, WOODS C, TOMI-ANDRINO C, REDDY BOMMAREDDY R, CHO BK, FELL DA, POOLMAN M, KING JR, WINZER K, TWYCROSS J, MINTON NP, 2022. A genome-scale metabolic model of Cupriavidus necator H16 integrated with TraDIS and transcriptomic data. reveals metabolic insights for biotechnological applications. PLOS Computational Biology. 18, e1010106
• TOMI-ANDRINO C, PANDELE A, WINZER K, KING J, RAHMAN R, KIM DH., 2022. Metabolic modeling-based drug repurposing in Glioblastoma. Scientific Reports. 12, 11189
• SALINAS A, MCGREGOR C, IRORERE V, ARENAS-LÓPEZ C, BOMMAREDDY RR, WINZER K, MINTON NP, KOVÁCS K, 2022. Metabolic engineering of Cupriavidus necator H16 for heterotrophic and autotrophic production of 3-hydroxypropionic acid Metabolic Engineering. 74, 178-190
• TOMI-ANDRINO C, NORMAN R, MILLAT T, SOUCAILLE P, WINZER K, BARRETT DA, KING J AND KIM D-H, 2021. Physicochemical and metabolic constraints for thermodynamics-based stoichiometric modelling under mesophilic growth conditions. PLOS Computational Biology. 17(1), e1007694
• SAFO L, ABDELRAZIG S, GROSSE-HONEBRINK A, MILLAT T, HENSTRA AM, NORMAN R, THOMAS NR, WINZER K, MINTON NP, KIM D-H and BARRETT DA, 2021. Quantitative Bioreactor Monitoring of Intracellular Bacterial Metabolites in Clostridium autoethanogenum using Liquid Chromatography-Isotope Dilution Mass Spectrometry ACS Omega. 6, 3518–13526
• GROSSE-HONEBRINK A, LITTLE GT, BEAN Z, HELDT D, CORNOCK RH, WINZER K, MINTON NP, GREEN E AND ZHANG Y, 2021. Development of Clostridium saccharoperbutylacetonicum as a whole cell biocatalyst for production of chirally pure (R)-1,3-butanediol. Frontiers in Bioengineering and Biotechnology. 9, 659895
• KOTTE A-K, SEVERN O, BEAN Z, SCHWARZ K, MINTON NP and WINZER K, 2020. RRNPP-type quorum sensing affects solvent formation and sporulation in Clostridium acetobutylicum Microbiology. 166, 579–592
• GILBERT J, PEARCY N, NORMAN R, MILLAT, T, WINZER K, KING J, MINTON NP and TWYCROSS J, 2019. Gsmodutils: A python based framework for test-driven genome scale metabolic model development. Bioinformatics. 35, 3397–3403
• NORMAN ROJ, MILLAT, T, SCHATSCHNEIDER S, HENSTRA AM, BREITKOPF R, PANDER B, ANNAN FJ, PIATEK P, HARTMAN HB, POOLMAN MG, FELL DA, WINZER K, MINTON NP and HODGEMAN C, 2019. A genome-scale model of Clostridium autoethanogenum reveals optimal bioprocess conditions for high-value chemical production from carbon monoxide. Engineering Biology. 3, 32-40
• ANNAN FJ, AL-SINAWI B, HUMPHREYS CM, NORMAN R, WINZER K, KÖPKE M, SIMPSON SD, MINTON NP and HENSTRA AM, 2019. Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum. Applied Microbiology and Biotechnology. 103, 4633-4648
• ARENAS-LÓPEZ C, LOCKER J, OROL D, WALTER F, BUSCHE T, KALINOWSKI J, MINTON NP, KOVÁCS K and WINZER K, 2019. The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16. Biotechnology for Biofuels. 12, 150
• PANDER B, HARRIS G, SCOTT DJ, WINZER K, KÖPKE M, SIMPSON SD, MINTON NP and HENSTRA AM, 2019. The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases. Applied Microbiology and Biotechnology. 103, 7275-7286
• WOODS C, HUMPHREYS CM, RODRIGUES RM, INGLE P, ROWE P, HENSTRA AM, KÖPKE M, SIMPSON SD, WINZER K and MINTON NP, 2019. A Novel Conjugal Donor Strain for Improved DNA transfer into Clostridium spp. Anaerobe. 59, 184-191
• LITTLE GT, EHSAAN M, ARENAS-LÓPEZ C, JAWED K, WINZER K, KOVÁCS K and MINTON NP, 2019. Complete Genome Sequence of Cupriavidus necator H16 (DSM 428) Microbiology Resource Announcements. 8, e00814-19
• SCHATSCHNEIDER, S, ABDELRAZIG, S, SAFO, L, HENSTRA, A, MILLAT, T, KIM, D-H, WINZER, K, MINTON, NP and BARRETT, DA, 2018. Quantitative isotope dilution high-resolution mass spectrometry analysis of multiple intracellular metabolites in Clostridium autoethanogenum using uniformly ¹³C-labelled standards derived from Spirulina. Analytical Chemistry. 90(7), 4470-4477
• LITTLE GT, WILLSON BJ, HEAP JT, WINZER K and MINTON NP, 2018. The butanol producing microbe Clostridium beijerinckii NCIMB 14988 manipulated using forward and reverse genetic tools. Biotechnol J. e1700711
• ALAGESAN S, MALYS N, HANKO EKR, EHSAAN M, WINZER K and MINTON NP, 2018. Functional genetic elements for controlling gene expression in Cupriavidus necator H16. Appl Environ Microbiol. 84, e00878-18
• NORMAN ROJ, MILLAT T, WINZER K, MINTON N and HODGMAN C, 2018. Progress towards platform chemical production using Clostridium autoethanogenum. Biochemical Society Transactions. 46(3), 523-535
• MILLAT T and WINZER K, 2017. Mathematical modelling of clostridial acetone-butanol-ethanol fermentation. Applied Microbiology and Biotechnology. 101(6), 2251-2271
• SHENG S, KOVÁCS K, WINZER K, ZHANG Y and MINTON NP, 2017. Development and implementation of rapid metabolic engineering tools for chemical and fuel production in Geobacillus thermoglucosidasius NCIMB 11955. Biotechnology for Biofuels. 10, 5
• LIEW F, HENSTRA AM, KӦPKE M, WINZER K, SIMPSON SD and MINTON NP, 2017. Metabolic Engineering of Clostridium autoethanogenum for Selective Alcohol Production. Metabolic Engineering. 40, 104–114
• POEHLEIN A, MONTOYA SOLANO JD, FLITSCH SK, KRABBEN P, WINZER K, REID SJ, JONES DT, GREEN E, MINTON NP, DANIEL R and DÜRRE P, 2017. Microbial solvent formation revisited by comparative genome analysis. Biotechnology for Biofuels. 10, 58
• MAGENNIS EP, FRANCINI N, MASTROTTO F, CATANIA R, REDHEAD M, FERNANDEZ-TRILLO F, BRADSHAW D, CHURCHLEY D, WINZER K, ALEXANDER C and MANTOVANI G, 2017. Polymers for Binding of the Gram-positive Oral Pathogen Streptococcus mutans. PLoS ONE. 12(7), e0180087
• EHSAAN M, KUIT W, ZHANG Y, CARTMAN S, HEAP J, WINZER K and MINTON NP, 2016. Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels. 9, 4
• LEIRE E, AMARAL SP, LOUZAO I, WINZER K, ALEXANDER C and FERNANDEZ-MEGIA E, 2016. Dendrimer mediated clustering of bacteria: Improved aggregation and evaluation of bacterial response and viability. Biomaterials Science. 4, 998-1006
• LIEW F, HENSTRA AM, WINZER K, KӦPKE M, SIMPSON SD and MINTON NP, 2016. Insights into CO2 fixation pathway of Clostridium autoethanogenum by targeted mutagenesis. mBio. 7, e00427-16
• WILSON B, KOVÁCS K, WILDING-STEELE T, MARKUS R, WINZER K and MINTON NP, 2016. Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels. 9, 109
• WALKER DJ, HEAP JT, WINZER K and MINTON NP, 2016. A genetic assay for gene essentiality in Clostridium. Anaerobe. 42, 40-43
• MAGENNIS EP, FERNANDEZ-TRILLO F, SUI C, SPAIN SG, BRADSHAW DJ, CHURCHLEY D, MANTOVANI G, WINZER K and ALEXANDER C, 2016. Corrigendum: Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling. Nature Materials. 15(2), 243
• LITTLE G., WINZER K. and MINTON N.P., 2015. Genome sequence of the solvent-producing Clostridium beijerincki strain 59B, isolated from Staffordshire garden soil. Genome Announcements. 3(2), e00108-15
• LOUZAO I., SUI C., WINZER K., FERNANDEZ-TRILLO F. and ALEXANDER C., 2015. Cationic polymer mediated bacterial clustering: Cell-adhesive properties of homo- and copolymers. European Journal of Pharmaceutics and Biopharmaceutics. 95, 47–62
• KUBIAK A., POEHLEIN A., BUDD P., KUEHNE S., WINZER K., THEYS J., LAMBIN P, DANIEL R. and MINTON N.P., 2015. Complete genome sequence of the non-pathogenic, soil dwelling bacterium Clostridium sporogenes strain NCIMB 10696. Genome Announcements. e00942-15
• HUMPHREYS, CM, MCLEAN, S, SCHATSCHNEIDER, S, MILLAT, T, HENSTRA, AM, ANNAN, FJ, BREITKOPF, R, PANDER, B, PIATEK, PM, ROWE, P, WICHLACZ, AT, WOODS, C, NORMAN, R, BLOM, J, GOESMAN, A, HODGMAN, C, BARRETT, D, THOMAS, NR, WINZER, K and MINTON, NP, 2015. Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium BMC Genomics. 16(1), 1058
• SCHWARZ K, ZHANG Y, KUIT W, EHSAAN M, KOVÁCS K, WINZER K and MINTON NP, 2015. New tools for the genetic modification of industrial Clostridia. In: MICHAEL E HIMMEL, ed., Direct Microbial Conversion of Biomass to Advanced Biofuels Part 4: Fuels from Bacteria. 241-289
• LEIRE, E., AMARAL, S. P., FERNANDEZ-TRILLO, F., CORREA, J., WINZER, K., ALEXANDER, C. and FERNANDEZ-MEGIA, E., 2015. Multifunctional Dendrimers as Platforms for the Development of New Microbicides Abstracts of the Interscience Conference on Antimicrobial Agents and Chemotherapy. 55, 345-346
• MAGENNIS E.P., FERNANDEZ-TRILLO F., SUI C., SPAIN S.S., CHURCHLEY D., MANTOVANI G, WINZER K. and ALEXANDER C., 2014. Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling. Nature Materials. 13, 748-755
• MILLAT T, VOIGT C, JANSSEN H, COOKSLEY CM, WINZER K, MINTON NP, BAHL H, FISCHER R and WOLKENHAUER O, 2014. Coenzyme A-transferase-independent butyrate re-assimilation in Clostridium acetobutylicum-evidence from a mathematical model. Applied Microbiology and Biotechnology. 98(21), 9059-72
• CARTMAN S.T., HEAP J.T., KUEHNE S., COOKSLEY C.M., EHSAAN M., WINZER K. and MINTON N.P., 2014. Clostridial Gene Tools. In: Systems Biology of Clostridium Imperial College Press. 21-55
• KELLY, M. L., VICKERS, R. J., WINZER, K., MINTON, N. P. and KUEHNE, S. A., 2014. The Effect of SMT19969, Fidaxomicin and Vancomycin on Sporulation in Clostridium difficile Abstracts of the Interscience Conference on Antimicrobial Agents and Chemotherapy. 54, F-241
• JABBARI S., KING J., MINTON N.P. and WINZER K., 2014. MODELLING AGR-DEPENDENT QUORUM SENSING IN GRAM-POSITIVE BACTERIA. In: P. DÜRRE, ed., Systems Biology of Clostridium. Imperial College Press. pp173-192
• KARAVOLOS M, WINZER K, WILLIAMS P and KHAN CMA, 2013. Pathogen Espionage: Multiple Bacterial Adrenergic Sensors Eavesdrop on Host Communication Systems. Molecular Microbiology. 87(3), 455-465
• JABBARI S, STEINER E, HEAP JT, WINZER K, MINTON NP and KING JR, 2013. The putative influence of the agr operon upon survival mechanisms used by Clostridium acetobutylicum. Mathematical Biosciences. 243(2), 223-39
• KOVÁCS K, WILLSON BJ, SCHWARZ K, HEAP JT, JACKSON A, BOLAM DN, WINZER K and MINTON NP, 2013. Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium. Biotechnology for Biofuels. 6, 117
• LUI, L.T., XUE, X., SUI, C., BROWN, A., PRITCHARD, D.I., HALLIDAY, N., WINZER, K., HOWDLE, S.M., FERNANDEZ-TRILLO, F., KRASNOGOR, N. and ALEXANDER, C., 2013. Bacteria clustering by polymers induces the expression of quorum-sensing-controlled phenotypes Nature Chemistry. 5(12), 1058-1065
• STEINER, E., SCOTT, J., MINTON, N.P. and WINZER, K., 2012. An agr quorum sensing system that regulates granulose formation and sporulation in Clostridium acetobutylicum Applied and Environmental Microbiology. 78(4), 1113-1122
• HEAP, J.T., EHSAAN, M., COOKSLEY, C.M., NG, Y.-H., CARTMAN, S.T., WINZER, K. and MINTON, N.P., 2012. Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker Nucleic Acids Research. 40(8), e59
• DARCH, S.E., WEST, S.A., WINZER, K. and DIGGLE, S.P., 2012. Density-dependent fitness benefits in quorum-sensing bacterial populations Proceedings of the National Academy of Sciences of the United States of America. 109(21), 8259-8263
• WEST S, WINZER K, GARDNER A and DIGGLE SP, 2012. Quorum sensing and the confusion about diffusion. Trends in Microbiology. 20, 586–594
• COOKSLEY, C.M., ZHANG, Y., WANG, H., REDL, S., WINZER, K. and MINTON, N.P., 2012. Targeted mutagenesis of the Clostridium acetobutylicum acetone-butanol-ethanol fermentation pathway Metabolic Engineering. 14(6), 630-641
• XUE, X., PASPARAKIS, G., HALLIDAY, N., WINZER, K., HOWDLE, S.M., CRAMPHORN, C.J., CAMERON, N.R., GARDNER, P.M., DAVIS, B.G., FERNÁNDEZ-TRILLO, F. and ALEXANDER, C., 2011. Synthetic polymers for simultaneous bacterial sequestration and quorum sense interference Angewandte Chemie International Edition. 50(42), 9852-9856
• KARAVOLOS, M.H., BULMER, D.M., SPENCER, H., RAMPIONI, G., SCHMALEN, I., BAKER, S., PICKARD, D., GRAY, J., FOOKES, M., WINZER, K., IVENS, A., DOUGAN, G., WILLIAMS, P. and ANJAM KHAN, C.M., 2011. Salmonella Typhi sense host neuroendocrine stress hormones and release the toxin haemolysin E EMBO Reports. 12(3), 252-258
• MINTON, NP and WINZER K, 2011. Solvent production-not to be sniffed at! Biobutanol: 21st century biofuel. Biochemist. 33, 8-13
• HALLIDAY, N M, HARDIE, K R, WILLIAMS, P, WINZER, K and BARRETT, D A, 2010. Quantitative liquid chromatography-tandem mass spectrometry profiling of activated methyl cycle metabolites involved in LuxS-dependent quorum sensing in Escherichia coli. Analytical Biochemistry. 403(1-2), 20-9
• SPENCER, H, KARAVOLOS, M H, BULMER, D M, ALDRIDGE, P, CHHABRA, S R, WINZER, K, WILLIAMS, P and KHAN, C M A, 2010. Genome-wide transposon mutagenesis identifies a role for host neuroendocrine stress hormones in regulating the expression of virulence genes in Salmonella. Journal of Bacteriology. 192(3), 714-24
• DOHERTY, N. C., SHEN, F., HALLIDAY, N. M., BARRETT, D. A., HARDIE, K. R., WINZER, K. and ATHERTON, J. C., 2010. Helicobacter pylori, LuxS is a key enzyme in cysteine provision through a reverse trassulfuration pathway Journal of Bacteriology. 192, 1184 - 1192
• COOKSLEY, CLARE M, DAVIS, IAN J, WINZER, KLAUS, CHAN, WENG C, PECK, MICHAEL W and MINTON, NIGEL P, 2010. Regulation of neurotoxin production and sporulation by a putative agrBD signaling system in proteolytic Clostridium botulinum. Applied and Environmental Microbiology. 76(13), 4448-60
• PERRETT, CHARLOTTE A, KARAVOLOS, MICHAIL H, HUMPHREY, SUZANNE, MASTROENI, PIETRO, MARTINEZ-ARGUDO, ISABEL, SPENCER, HANNAH, BULMER, DAVID, WINZER, KLAUS, MCGHIE, EMMA, KORONAKIS, VASSILIS, WILLIAMS, P, KHAN, C M A and JEPSON, M A, 2009. LuxS-based quorum sensing does not affect the ability of Salmonella enterica serovar Typhimurium to express the SPI-1 type 3 secretion system, induce membrane ruffles, or invade epithelial cells. Journal of Bacteriology. 191(23), 7253-9
• HEURLIER, K., VENDEVILLE, A., HALLIDAY, N., GREEN, A., WINZER, K., TANG, C.M. and HARDIE, K.R., 2009. Growth deficiencies of Neisseria meningitidis pfs and luxS mutants are not due to inactivation of quorum sensing Journal of Bacteriology. 191(4), 1293-1302
• HOLMES, K., TAVENDER, T.J., WINZER, K., WELLS, J.M. and HARDIE, K.R., 2009. AI-2 does not function as a quorum sensing molecule in Campylobacter jejuni during exponential growth in vitro BMC Microbiology. 9, 214
• GARDNER, P.M., WINZER, K. and DAVIS, B.G., 2009. Sugar synthesis in a protocellular model leads to a cell signalling response in bacteria Nature Chemistry. 1(5), 377-383
• KARAVOLOS, M H, BULMER, D M, WINZER, K, WILSON, M, MASTROENI, P, WILLIAMS, P and KHAN, C M A, 2008. LuxS affects flagellar phase variation independently of quorum sensing in Salmonella enterica serovar typhimurium. Journal of Bacteriology. 190(2), 769-71
• JENSEN, R.O., WINZER, K., CLARKE, S.R., CHAN, W.C. and WILLIAMS, P., 2008. Differential recognition of Staphylococcus aureus quorum-sensing signals depends on both extracellular loops 1 and 2 of the transmembrane sensor AgrC Journal of Molecular Biology. 381(2), 300-309
• KARAVOLOS, M H, SPENCER, H, BULMER, D M, THOMPSON, A, WINZER, K, WILLIAMS, P, HINTON, J C D and KHAN, C M ANJAM, 2008. Adrenaline modulates the global transcriptional profile of Salmonella revealing a role in the antimicrobial peptide and oxidative stress resistance responses. BMC Genomics. 9, 458
• TAVENDER, TIMOTHY J, HALLIDAY, NIGEL M, HARDIE, KIM R and WINZER, KLAUS, 2008. LuxS-independent formation of AI-2 from ribulose-5-phosphate. BMC Microbiology. 8, 98
• WILLIAMS, PAUL, WINZER, KLAUS, CHAN, WENG C and CÁMARA, MIGUEL, 2007. Look who's talking: communication and quorum sensing in the bacterial world. Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 362(1483), 1119-34
• DIGGLE, S.P., LUMJIAKTASE, P., DIPILATO, F., WINZER, K., KUNAKORN, M., BARRETT, D.A., CHHABRA, S.R., CAMARA, M. and WILLIAMS, P., 2006. Functional genetic analysis reveals a 2-alkyl-4-quinolone signaling system in the human pathogen Burkholderia pseudomallei and related bacteria Chemistry & Biology. 13(7), 701-710
• DIGGLE, S.P., STACEY, R.E., DODD, C., CÁMARA, M., WILLIAMS, P. and WINZER, K., 2006. The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa Environmental Microbiology. 8(6), 1095-1104
• DOHERTY, N., HOLDEN, M.T.G., QAZI, S.N., WILLIAMS, P. and WINZER, K., 2006. Functional analysis of luxS in Staphylococcus aureus reveals a role in metabolism but not quorum sensing Journal of Bacteriology. 188(8), 2885-2897
• WAND, M.E., SOCKETT, R.E., EVANS, K.J., DOHERTY, N., SHARP, P.M., HARDIE, K.R. and WINZER, K., 2006. Helicobacter pylori FlhB function: the FlhB C-terminal homologue HP1575 acts as a "spare part" to permit flagellar export when the HP0770 FlhB_CC domain is deleted Journal of Bacteriology. 188(21), 7531-7541
• VENDEVILLE, A., WINZER, K., HEURLIER, K., TANG, C. M. and HARDIE, K. R., 2005. Making `sense' of metabolism: autoinducer-2, LuxS and pathogenic bacteria Nature Reviews: Microbiology. VOL 3(NUMB 5), 383-396
• DIGGLE, S.P., WINZER, K., CHHABRA, S.R., WORRALL, K.E., CÁMARA, M. and WILLIAMS, P., 2003. The Pseudomonas aeruginosa quinolone signal molecule overcomes the cell density-dependency of the quorum sensing hierarchy, regulates rhl-dependent genes at the onset of stationary phase and can be produced in the absence of LasR Molecular Microbiology. 50(1), 29-43
• WINZER,K., HARDIE,K.R. and WILLIAMS,P., 2003. LuxS and autoinducer-2: their contribution to quorum sensing and metabolism in bacteria. In: LASKIN, A.I., BENNETT, J.W. and GADD, G.M., eds., Advances in applied microbiology Vol. 53. Elsevier Academic Press. 291-396
• WINZER, K. and WILLIAMS, P., 2003. Escherichia coli gets the message Nature Medicine. 9(9), 1118-1119
• HARDIE, K.R., COOKSLEY, C., GREEN, A.D. and WINZER, K., 2003. Autoinducer 2 activity in Escherichia coli culture supernatants can be actively reduced despite maintenance of an active synthase, LuxS Microbiology. 149(3), 715-728
• WINZER, K., HARDIE, K.R., BURGESS, N., DOHERTY, N., KIRKE, D., HOLDEN, M.T.G., LINFORTH, R., CORNELL, K.A., TAYLOR, A.J., HILL, P.J. and WILLIAMS P., 2002. LuxS: its role in central metabolism and the in vitro synthesis of 4-hydroxy-5-methyl-3(2H)-furanone Microbiology. 148(4), 909-922
• DIGGLE, S.P., WINZER, K., LAZDUNSKI, A., WILLIAMS, P. and CÁMARA, M., 2002. Advancing the quorum in Pseudomonas aeruginosa: MvaT and the regulation of N-acylhomoserine lactone production and virulence gene expression Journal of Bacteriology. 184(10), 2576-2586
• WINZER, K., HARDIE, K.R. and WILLIAMS, P., 2002. Bacterial cell-to-cell communication: sorry, can't talk now - gone to lunch! Current Opinion in Microbiology. 5(2), 216-222
• BURGESS,N.A., KIRKE,D.F., WILLIAMS,P., WINZER,K., HARDIE,K.R., MEYER,N.L., ADUSE-OPOKU,J., CURTIS,M.A. and CAMARA,M., 2002. LuxS-dependent quorum sensing in Porphyromonas gingivalis modulates protease and haemagglutinin activities but is not essential for virulence Microbiology. 148(3), 763-772
• WINZER, K., SUN, Y.-H., GREEN, A., DELORY, M., BLACKLEY, D., HARDIE, K.R., BALDWIN, T.J. and TANG, C.M., 2002. Role of Neisseria meningitidis luxS in cell-to-cell signaling and bacteremic infection Infection and Immunity. 70(4), 2245-2248
• QAZI, S.N.A., COUNIL, E., MORRISSEY, J., REES, C.E.D., COCKAYNE, A., WINZER, K., CHAN, W.C., WILLIAMS, P. and HILL, P.J., 2001. agr expression precedes escape of internalized Staphylococcus aureus from the host endosome Infection and Immunity. 69(11), 7074-7082
• WINZER, K. and WILLIAMS, P., 2001. Quorum sensing and the regulation of virulence gene expression in pathogenic bacteria International Journal of Medical Microbiology. 291(2), 131-143
• WINZER, K., FALCONER, C., GARBER, N.C., DIGGLE, S.P., CAMARA, M. and WILLIAMS, P., 2000. The Pseudomonas aeruginosa lectins PA-IL and PA-IIL are controlled by quorum sensing and by RpoS Journal of Bacteriology. 182(22), 6401-6411
• WILLIAMS, P, CAMARA, M, HARDMAN, A, SWIFT, S, MILTON, D, HOPE, V J, WINZER, K, MIDDLETON, B, PRITCHARD, D I and BYCROFT, B W, 2000. Quorum sensing and the population-dependent control of virulence. Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 355(1397), 667-80
• WINZER, K, LORENZ, K, ZICKNER, B and DÜRRE, P, 2000. Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792. Journal of molecular microbiology and biotechnology. 2(4), 531-41
• EXTERNBRINK, T, HUJER, S, WINZER, K and DÜRRE, P, 2000. Sequence analysis of the atp operon of Clostridium acetobutylicum DSM 792 encoding the F0F1 ATP synthase. DNA sequence : the journal of DNA sequencing and mapping. 11(1-2), 109-18
• WINZER, K, LORENZ, K and DÜRRE, P, 1997. Acetate kinase from Clostridium acetobutylicum: a highly specific enzyme that is actively transcribed during acidogenesis and solventogenesis. Microbiology (Reading, England). 143 ( Pt 10), 3279-86
• DÜRRE, P, FISCHER, R J, KUHN, A, LORENZ, K, SCHREIBER, W, STÜRZENHOFECKER, B, ULLMANN, S, WINZER, K and SAUER, U, 1995. Solventogenic enzymes of Clostridium acetobutylicum: catalytic properties, genetic organization, and transcriptional regulation. FEMS microbiology reviews. 17(3), 251-62