Contact

• workRoom B13 Clinical Sciences Building
  Nottingham City Hospital
  Hucknall Road
  Nottingham
  NG5 1PB
  UK
• work+44 (0) 115 82 31106
• Chitra.Joseph@nottingham.ac.uk

Research Summary

I am a Research Fellow within the Centre for Respiratory Research/ Nottingham BRC in Translational Medical Sciences.

My research is focused on the study of cellular and molecular pathology mechanisms underlying chronic lung diseases (including idiopathic pulmonary fibrosis, asthma, COPD) and viral infections. I also participate in studies evaluating computational image analysis to quantify fibrosis/cellularity, expression profiling and reverse phase proteomics quantifying biomarkers.

Expertise Summary

Isolation, culture and characterisation of a variety of primary cells from human and animal tissue;

Molecular genetics, tissue microarrays

RNAScope, Pyrosequencing

Image analysis, Immunohistochemistry, immunocytochemistry, immunofluorescence and histological staining

Western blotting and electrophoresis

Recent Publications

• KARIRI, YOUSIF, TOSS, MICHAEL S., ALSALEEM, MANSOUR, ELSHARAWY, KHLOUD A., JOSEPH, CHITRA, MONGAN, NIGEL P., GREEN, ANDREW R. and RAKHA, EMAD A., 2022. Ubiquitin-conjugating enzyme 2C (UBE2C) is a poor prognostic biomarker in invasive breast cancer BREAST CANCER RESEARCH AND TREATMENT.
• JOSEPH, CHITRA and TATLER, AMANDA L., 2022. Pathobiology of Airway Remodeling in Asthma: The Emerging Role of Integrins JOURNAL OF ASTHMA AND ALLERGY. 15, 595-610
• JOSEPH, CHITRA, ALSALEEM, MANSOUR A., TOSS, MICHAEL S., KARIRI, YOUSIF A., ALTHOBITI, MARYAM, ALSAEED, SAMI, ALJOHANI, ABRAR I., NARASIMHA, PAVAN L., MONGAN, NIGEL P., GREEN, ANDREW R. and RAKHA, EMAD A., 2021. The ITIM-Containing Receptor: Leukocyte-Associated Immunoglobulin-Like Receptor-1 (LAIR-1) Modulates Immune Response and Confers Poor Prognosis in Invasive Breast Carcinoma CANCERS. 13(1),
• KARIRI, YOUSIF A., ALSALEEM, MANSOUR, JOSEPH, CHITRA, ALSAEED, SAMI, ALJOHANI, ABRAR, SHIINO, SHO, MOHAMMED, OMAR J., TOSS, MICHAEL S., GREEN, ANDREW R. and RAKHA, EMAD A., 2021. The prognostic significance of interferon-stimulated gene 15 (ISG15) in invasive breast cancer BREAST CANCER RESEARCH AND TREATMENT. 185(2), 293-305

• View all publications

Associate Fellowship of Higher Education Academy (AFHEA)

• KARIRI, YOUSIF, TOSS, MICHAEL S., ALSALEEM, MANSOUR, ELSHARAWY, KHLOUD A., JOSEPH, CHITRA, MONGAN, NIGEL P., GREEN, ANDREW R. and RAKHA, EMAD A., 2022. Ubiquitin-conjugating enzyme 2C (UBE2C) is a poor prognostic biomarker in invasive breast cancer BREAST CANCER RESEARCH AND TREATMENT.
• JOSEPH, CHITRA and TATLER, AMANDA L., 2022. Pathobiology of Airway Remodeling in Asthma: The Emerging Role of Integrins JOURNAL OF ASTHMA AND ALLERGY. 15, 595-610
• JOSEPH, CHITRA, ALSALEEM, MANSOUR A., TOSS, MICHAEL S., KARIRI, YOUSIF A., ALTHOBITI, MARYAM, ALSAEED, SAMI, ALJOHANI, ABRAR I., NARASIMHA, PAVAN L., MONGAN, NIGEL P., GREEN, ANDREW R. and RAKHA, EMAD A., 2021. The ITIM-Containing Receptor: Leukocyte-Associated Immunoglobulin-Like Receptor-1 (LAIR-1) Modulates Immune Response and Confers Poor Prognosis in Invasive Breast Carcinoma CANCERS. 13(1),
• KARIRI, YOUSIF A., ALSALEEM, MANSOUR, JOSEPH, CHITRA, ALSAEED, SAMI, ALJOHANI, ABRAR, SHIINO, SHO, MOHAMMED, OMAR J., TOSS, MICHAEL S., GREEN, ANDREW R. and RAKHA, EMAD A., 2021. The prognostic significance of interferon-stimulated gene 15 (ISG15) in invasive breast cancer BREAST CANCER RESEARCH AND TREATMENT. 185(2), 293-305
• JOHN, ALISON E., JOSEPH, CHITRA, JENKINS, GISLI and TATLER, AMANDA L., 2021. COVID-19 and pulmonary fibrosis: A potential role for lung epithelial cells and fibroblasts IMMUNOLOGICAL REVIEWS.
• ALTHOBITI, MARYAM, EL-SHARAWY, KHLOUD A., JOSEPH, CHITRA, ALESKANDARANY, MOHAMMED, TOSS, MICHAEL S., GREEN, ANDREW R. and RAKHA, EMAD A., 2021. Oestrogen-regulated protein SLC39A6: a biomarker of good prognosis in luminal breast cancer BREAST CANCER RESEARCH AND TREATMENT. 189(3), 621-630
• BUI, LINH T., WINTERS, NICHELLE I., CHUNG, MEI-I, JOSEPH, CHITRA, GUTIERREZ, AUSTIN J., HABERMANN, ARUN C., ADAMS, TAYLOR S., SCHUPP, JONAS C., POLI, SERGIO, PETER, LANCE M., TAYLOR, CHASE J., BLACKBURN, JESSICA B., RICHMOND, BRADLEY W., NICHOLSON, ANDREW G., RASSL, DORIS, WALLACE, WILLIAM A., ROSAS, IVAN O., JENKINS, R. GISLI, KAMINSKI, NAFTALI, KROPSKI, JONATHAN A. and BANOVICH, NICHOLAS E., 2021. Chronic lung diseases are associated with gene expression programs favoring SARS-CoV-2 entry and severity NATURE COMMUNICATIONS. 12(1),
• MICHAEL S. TOSS, ASIMA ABIDI, DOROTHEA LESCHE, CHITRA JOSEPH, SAKSHI MAHALE, HUGO SAUNDERS, TANJINA KADER, ISLAM M. MILIGY, ANDREW R. GREEN, KYLIE L. GORRINGE & EMAD A. RAKHA, 2020. The prognostic significance of immune microenvironment in breast ductal carcinoma in situ British Journal of Cancer.
• CAIRNS, JENNIFER T., HABGOOD, ANTHONY, EDWARDS-PRITCHARD, ROCHELLE C., JOSEPH, CHITRA, JOHN, ALISON E., WILKINSON, CHLOE, STEWART, IAIN D., LESLIE, JACK, BLAXALL, BURNS C., SUSZTAK, KATALIN, ALBERTI, SIEGFRIED, NORDHEIM, ALFRED, OAKLEY, FIONA, JENKINS, GISLI and TATLER, AMANDA L., 2020. Loss of ELK1 has differential effects on age-dependent organ fibrosis INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY. 120,
• JOSEPH C, ALSALEEM M, ORAH N, NARASIMHA PL, MILIGY IM, KUROZUMI S, ELLIS IO, MONGAN NP, GREEN AR and RAKHA EA, 2020. Elevated MMP9 expression in breast cancer is a predictor of shorter patient survival. Breast cancer research and treatment. 182(2), 267-282
• AGUIAR, JENNIFER A., TREMBLAY, BENJAMIN J-M, MANSFIELD, MICHAEL J., WOODY, OWEN, LOBB, BRIALLEN, BANERJEE, ARINJAY, CHANDIRAMOHAN, ABIRAM, TIESSEN, NICHOLAS, CAO, QUYNH, DVORKIN-GHEVA, ANNA, REVILL, SPENCER, MILLER, MATTHEW S., CARLSTEN, CHRISTOPHER, ORGAN, LOUISE, JOSEPH, CHITRA, JOHN, ALISON, HANSON, PAUL, AUSTIN, RICHARD C., MCMANUS, BRUCE M., JENKINS, GISLI, MOSSMAN, KAREN, ASK, KJETIL, DOXEY, ANDREW C. and HIROTA, JEREMY A., 2020. Gene expression and in situ protein profiling of candidate SARS-CoV-2 receptors in human airway epithelial cells and lung tissue EUROPEAN RESPIRATORY JOURNAL. 56(3),
• KARIRI, YOUSIF A., ALESKANDARANY, MOHAMMED A., JOSEPH, CHITRA, KUROZUMI, SASAGU, MOHAMMED, OMAR J., TOSS, MICHAEL S., GREEN, ANDREW R. and RAKHA, EMAD A., 2020. Molecular Complexity of Lymphovascular Invasion: The Role of Cell Migration in Breast Cancer as a Prototype PATHOBIOLOGY. 87(4), 218-231
• ALTHOBITI, MARYAM, EL ANSARI, ROKAYA, ALESKANDARANY, MOHAMMED, JOSEPH, CHITRA, TOSS, MICHAEL S., GREEN, ANDREW R. and RAKHA, EMAD A., 2020. The prognostic significance of ALDH1A1 expression in early invasive breast cancer HISTOPATHOLOGY. 77(3), 437-447
• ALJOHANI, ABRAR, I, JOSEPH, CHITRA, KUROZUMI, SASAGU, MOHAMMED, OMAR J., MILIGY, ISLAM M., GREEN, ANDREW R. and RAKHA, EMAD A., 2020. Myxovirus resistance 1 (MX1) is an independent predictor of poor outcome in invasive breast cancer BREAST CANCER RESEARCH AND TREATMENT. 181(3), 541-551
• ALTHOBITI, MARYAM, MUFTAH, ABIR A., ALESKANDARANY, MOHAMMED A., JOSEPH, CHITRA, TOSS, MICHAEL S., GREEN, ANDREW and RAKHA, EMAD, 2020. The prognostic significance of BMI1 expression in invasive breast cancer is dependent on its molecular subtypes BREAST CANCER RESEARCH AND TREATMENT. 182(3), 581-589
• JOSEPH, CHITRA, ALSALEEM, MANSOUR, ORAH, NNAMDI, NARASIMHA, PAVAN L., MILIGY, ISLAM M., KUROZUMI, SASAGU, ELLIS, IAN O., MONGAN, NIGEL P., GREEN, ANDREW R. and RAKHA, EMAD A., 2020. Elevated MMP9 expression in breast cancer is a predictor of shorter patient survival BREAST CANCER RESEARCH AND TREATMENT. 182(2), 267-282
• ALSALEEM, MANSOUR A., BALL, GRAHAM, TOSS, MICHAEL S., RAAFAT, SARA, ALESKANDARANY, MOHAMMED, JOSEPH, CHITRA, OGDEN, ANGELA, BHATTARAI, SHRISTI, RIDA, PADMASHREE C. G., KHANI, FRANCESCA, DAVIS, MELISSA, ELEMENTO, OLIVIER, ANEJA, RITU, ELLIS, IAN O., GREEN, ANDREW, MONGAN, NIGEL P. and RAKHA, EMAD, 2020. A novel prognostic two-gene signature for triple negative breast cancer MODERN PATHOLOGY. 33(11), 2208-2220
• KUROZUMI, SASAGU, JOSEPH, CHITRA, SONBUL, SULTAN, ALSAEED, SAMI, KARIRI, YOUSIF, ALJOHANI, ABRAR, RAAFAT, SARA, ALSALEEM, MANSOUR, OGDEN, ANGELA, JOHNSTON, SIMON J, ALESKANDARANY, MOHAMMED A, FUJII, TAKAAKI, SHIRABE, KEN, CALDAS, CARLOS, ASHANKYTY, IBRAHEEM, DALTON, LESLIE, ELLIS, IAN O, DESMEDT, CHRISTINE, GREEN, ANDREW R, MONGAN, NIGEL P and RAKHA, EMAD A, 2019. A key genomic subtype associated with lymphovascular invasion in invasive breast cancer. British journal of cancer. 120(12), 1129-1136
• KUROZUMI, SASAGU, JOSEPH, CHITRA, RAAFAT, SARA, SONBUL, SULTAN, KARIRI, YOUSIF, ALSAEED, SAMI, PIGERA, MARIAN, ALSALEEM, MANSOUR, NOLAN, CHRISTOPHER C, JOHNSTON, SIMON J, ALESKANDARANY, MOHAMMED A, OGDEN, ANGELA, FUJII, TAKAAKI, SHIRABE, KEN, MARTIN, STEWART G, ALSHANKYTY, IBRAHEEM, MONGAN, NIGEL P, ELLIS, IAN O, GREEN, ANDREW R and RAKHA, EMAD A, 2019. Utility of ankyrin 3 as a prognostic marker in androgen-receptor-positive breast cancer. Breast cancer research and treatment. 176(1), 63-73
• ALSALEEM, MANSOUR, TOSS, MICHAEL S., JOSEPH, CHITRA, ALESKANDARANY, MOHAMMED, KUROZUMI, SASAGU, ALSHANKYTY, IBRAHIM, OGDEN, ANGELA, RIDA, PADMASHREE C. G., ELLIS, IAN O., ANEJA, RITU, GREEN, ANDREW R., MONGAN, NIGEL P. and RAKHA, EMAD A., 2019. The molecular mechanisms underlying reduced E-cadherin expression in invasive ductal carcinoma of the breast: high throughput analysis of large cohorts MODERN PATHOLOGY. 32(7), 967-976
• KARIRI YA, JOSEPH C, KUROZUMI S, TOSS MS, ALSALEEM M, RAAFAT S, MONGAN NP, ALESKANDARANY MA, GREEN AR and RAKHA EA, 2019. Prognostic significance of KN motif and ankyrin repeat domains 1 (KANK1) in invasive breast cancer. Breast cancer research and treatment. 179, 79–90
• ALJOHANI AI, TOSS MS, KUROZUMI S, JOSEPH C, ALESKANDARANY MA, MILIGY IM, ANSARI RE, MONGAN NP, ELLIS IO, GREEN AR and RAKHA EA, 2019. The prognostic significance of wild-type isocitrate dehydrogenase 2 (IDH2) in breast cancer. Breast cancer research and treatment. (In Press.)
• JOSEPH C, AL-IZZI S, ALSALEEM M, KUROZUMI S, TOSS MS, ARSHAD M, GOH FQ, ALSHANKYTY IM, ALESKANDARANY MA, ALI S, ELLIS IO, MONGAN NP, GREEN AR and RAKHA EA, 2019. Retinoid X receptor gamma (RXRG) is an independent prognostic biomarker in ER-positive invasive breast cancer. British journal of cancer. 121(9), 776-785
• KUROZUMI S, JOSEPH C, SONBUL S, GORRINGE KL, PIGERA M, ALESKANDARANY MA, DIEZ-RODRIGUEZ M, NOLAN CC, FUJII T, SHIRABE K, KUWANO H, STORR S, MARTIN SG, ELLIS IO, GREEN AR and RAKHA EA, 2018. Clinical and biological roles of Kelch-like family member 7 in breast cancer: a marker of poor prognosis. Breast cancer research and treatment. 170(3), 525-533
• JOSEPH C, MACNAMARA O, CRAZE M, RUSSELL R, PROVENZANO E, NOLAN CC, DIEZ-RODRIGUEZ M, SONBUL SN, ALESKANDARANY MA, GREEN AR, RAKHA EA, ELLIS IO and MUKHERJEE A, 2018. Mediator complex (MED) 7: a biomarker associated with good prognosis in invasive breast cancer, especially ER+ luminal subtypes. British journal of cancer. 118(8), 1142-1151
• SONBUL, S. N., ALESKANDARANY, M. A., KUROZUMI, S., JOSEPH, C., TOSS, M. S., DIEZ-RODRIGUEZ, M., NOLAN, C. C., MUKHERJEE, A., MARTIN, S., CALDAS, C., ELLIS, I. O., GREEN, A. R. and RAKHA, E. A., 2018. Saccharomyces cerevisiae-like 1 (SEC14L1) is a prognostic factor in breast cancer associated with lymphovascular invasion Mod Pathol. 31(11), 1675-1682
• KUROZUMI, S., JOSEPH, C., SONBUL, S., ALESKANDARANY, M. A., PIGERA, M., ALSALEEM, M., ALSAEED, S., KARIRI, Y., NOLAN, C. C., DIEZ-RODRIGUEZ, M., JOHNSTON, S., MONGAN, N. P., FUJII, T., SHIRABE, K., MARTIN, S. G., ELLIS, I. O., GREEN, A. R. and RAKHA, E. A., 2018. Clinicopathological and prognostic significance of Ras association and pleckstrin homology domains 1 (RAPH1) in breast cancer Breast Cancer Res Treat. 172(1), 61-68
• JOSEPH C, ARSHAD M, KUROZOMI S, ALTHOBITI M, MILIGY IM, AL-IZZI S, TOSS MS, GOH FQ, JOHNSTON SJ, MARTIN SG, ELLIS IO, MONGAN NP, GREEN AR and RAKHA EA, 2018. Overexpression of the cancer stem cell marker CD133 confers a poor prognosis in invasive breast cancer. Breast cancer research and treatment. 174(2), 387-399
• CHRYSANTHOU E, GORRINGE KL, JOSEPH C, CRAZE M, NOLAN CC, DIEZ-RODRIGUEZ M, GREEN AR, RAKHA EA, ELLIS IO and MUKHERJEE A, 2017. Phenotypic characterisation of breast cancer: the role of CDC42. Breast cancer research and treatment.
• MUKHERJEE, ABHIK, JOSEPH, CHITRA, CRAZE, MADELEINE, CHRYSANTHOU, ELENI and ELLIS, IAN O., 2015. The role of BUB and CDC proteins in low-grade breast cancers LANCET. 385, 72-72
• JOSEPH, C., HUNTER, M.G., SINCLAIR, K.D. and ROBINSON, R.S., 2012. The expression, regulation and function of secreted protein, acidic, cysteine-rich (sparc) in the follicle-luteal transition Reproduction. 144(3), 361-372