Image Analysis
Software
There is a range of equipment for image analysis, deconvolution, tracking and colocalisation:
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Volocity is imaging software used with 2D and 3D images. It has many features including: colocalisation, deconvolution (‘fast’ to improve an image for presentation and ‘iterative’ to sharpen an image for analysis) and movie sequence capabilities. There are also many other options including: collating and viewing data graphically, creating 3D representations of data and tracking specific objects through a time sequence.
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Huygens Professional is tailored for deconvolution/restoration of microscopic images. Our version also has modules for advanced object finding/tracking, 3D rendering, chromatic aberration correction and colocalisation.
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Fiji is an image processing package. It can be described as a "batteries-included" distribution of ImageJ (and ImageJ2), bundling Java, Java3D and a lot of plugins organized into a coherent menu structure.
It is freely available.
Applications
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Deconvolution of data stacks.
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Measurement of colocalisation.
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Quantification of areas, volumes and regions of interest.
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Cell/molecule tracking in 3D and 4D.
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Image analysis and quantification of areas, volumes, ROIs, lengths, thresholding, object size and distribution, and 3D image rendering and animation.