SLIM Image Gallery

0aa_W3CMSNP2a__Goulding_print_cropWeb

Uploaded: Oct 04, 2017
Description: 1st Place, Joelle GouldingThe image is a human embryonic stem cell derived cardiomyocyte which has been labelled for alpha-actinin and the nuclear stain Hoechst. Alpha-actinin is a cardiomyocyte marker and is used to establish correct differentiation of the stem cell. The image was taken on the Zeiss LSM 710 confocal microscope.What it means to me…….'Silver Linings' despite my experiment not working the resultant image is eye-catching and beautiful.

0bRuiter_3T3 cells_Printweb

Uploaded: Oct 04, 2017
Description: 2nd Place, Floor RuiterHereby, my best image I have obtained from SLIMs on the superresultion Zeiss Elyra in CBS. Short description: Red fluorescence protein expressing 3T3 fibroblast on PLA/thermo-responsive fluorescent labelled electrospun fibrous scaffold. The green expression shows the distribution of the thermo-responsive polymer within the fibres. This image shows that the cells don't mind the polymer and grow nicely on the fibres.

0cSLIM Image Competition - Othman_Print

Uploaded: Oct 04, 2017
Description: 3rd Place, Othman OthmanThe attached file which is one of the best images I took during my PhD study using the Zeiss LSM 710 confocal microscope at 1 Airy Unit (AU) with the following excitation/emission filters: DAPI/Hoechst: 405 nm/420 – 480 nm, YFP: 488 nm/505 – 550 nm, Alexa Fluor 568: 561 nm/575 – 615 nm.The image is a 40x image of sagittal brain sections (20 µm) of 6-months old APPswe/PS1?E9/YFP-H mouse show cortical neurons with severe axonal dystrophies co-localized with amyloid-ß plagues.

533P-356P YES TCRed Pha~ 04 CH-3

Uploaded: Oct 04, 2017
Description: Cheng HuaitaoThe thrid one is DEF6 533-536 mutant exhibited in activated T cell (Jurkat T cell line) that conjugated with a B cell (Raji B cell line). These two cells were stained by phallodine as well that indicated the immunological synapse (the junction between T and B cells). It can be seen, DEF6 533-536 mutant translocated in this junction.

6mA vs 5mC in LN18 mitotic cells with DAPI as counterstain

Uploaded: Oct 04, 2017
Description: Abdulkadir AbakirWhat is the image?Co-staining of human glioma cell line (LN-18) for 6mA (N6-adenine methylation) (green) and 5mC (5-methylcytosine) (red) arrested during mitotic metaphase. In addition, DAPI was used as a nuclearstain. The image was acquired with a Zeiss Elyra Super Resolution confocal Microscope in CBS.What means to me?This is the first ever demonstration of 6mA staining in mitotic metaphase of cancer cells. We arecurrently addressing the functional role of this modification on cell cycle. Basically this imageprovided me with the essential proof of principle for my PhD project.

image 1 YA

Uploaded: Oct 04, 2017
Description: Yasir AlmuhannaThis is an image of human macrophage cells stained in blue (DNA) and red (Actin) co-cultured with Pseudomonas aeruginosa biofilms (green). Macrophage work as the guardians of our body that detect invading microorganisms. By forming biofilms, the bacteria is no longer visible for macrophage.

image 2 YA

Uploaded: Oct 04, 2017
Description: Yasir AlmuhannaThis is an image of Pseudomonas aeruginosa biofilms formation process. Biofilms are bacterial communities formed by many individual bacteria embedded in an extracellular matrix. The image shows how bacteria stained (green and blue) moves towards specific locations as if it follows a lead that will guide them towards these specific locations. The image represents the organization and the team work in this tiny world!

image 3 YA

Uploaded: Oct 04, 2017
Description: Yasir AlmuhannaBiofilms are bacterial communities embedded in an extracellular matrix. In this image, the bacterial community stained in green, red, and blue is moving in streamed motion to disperse the bacteria in another area of the surface.

Microalgae_with scale

Uploaded: Oct 04, 2017
Description: Patchaniya EakpetchVisualization of the disrupted microalgae (C.reinhardtii) by micro-cavitation using confocal microscopy, single optical slices are shown. Cells were stained with Nile Red fluorescent dye. The chlorophyll is shown in green; Oil droplets are yellow in the overview and red in the inserts. Inserts are channel unmixed zoomed images of the microalgae. (Scale bars = 10 µm and 5 µm for inserts).

Neurons - NT

Uploaded: Oct 04, 2017
Description: Nel TaylorImage of neurons in the dentate gyrus of the hippocampus in a 56 day old male mouse with transgenic overexpression of NMNAT1. Cells stained for doublecortin (DCX), a marker of newly developing neuronal cells and neurogenesis levels.Image obtained with the Axioplan/Axiophot brightfield microscope

PHDH2-ALL10 No TCRed ~03 CH-2

Uploaded: Oct 04, 2017
Description: Cheng HuaitaoDEF6 PHDH2-All-10 mutant expressed in resting T cells (Jurkat T cell line). They both localised indicated cell protrusions. These cells were also stained by red phalloidine that binds to F-actin.

SLIM competition 2017

Uploaded: Oct 04, 2017
Description: Liaque LatifHere is a Cross section of a Porcine coronary artery stained with three dyes: Blue = DAPI, Red = Smooth muscle Beta actin and Green = P2Y14 receptor. This was imaged in SLIM for preliminary data staining for the first time the presence of a P2Y14 receptor on the epithelial cells of a porcine coronary artery and was submitted as a part of a successful grant application resulting in the recruitment of a PhD student.

SLIM IMAGE_U.B._4181919

Uploaded: Oct 04, 2017
Description: Ujjal BoseRatiometrically analysed image of a cluster of ovarian cancer cells (SK-OV-3) in monolayer,cultured in physiological extracellular pH (pHe = 7.42) and oxygen tension (0.21 atm partial pressureof oxygen, PO2). The variability in distribution of intracellular pH (pHi) in the imaged live cancer cellswas determined and mapped on to the monolayer of SK-OV-3 cells. The ratio image recorded a pHiof 7.47 in the cancer cells under the given conditions.he image was part of PhD study under the supervision of Professor Raheela Khan (School of Medicine,University of Nottigham).The original signals (Figure 2.) acquired using the Olympus Deltavision wide field microscope wereratiometrically analysed to produce the above image (Figure 1) that provides a detailed informationon the distribution of protons in live ovarian cancer cells. This data generated important informationon the pHi distribution and its mapping in live cancer cells during experimental interventions. The datareveals the inhomogeneity in the pHidistribution that exists in cancer cells also known to effect cellularmetabolism under the influence of a physiological microenvironment. This opened avenues ofCytosolic regions with low pH Cell membrane withhigh pHAcidic intracellular pHhotspotsFigure 1: Ratiometrically analysed image of a cluster of ovarian cancer cells (SK-OV-3) in monolayer,cultured in physiological extracellular pH (pHe = 7.42) and oxygen tension (0.21 atm partial pressureof oxygen, PO2). The variability in distribution of intracellular pH (pHi) in the imaged live cancer cellswas determined and mapped on to the monolayer of SK-OV-3 cells. The ratio image recorded a pHiof 7.47 in the cancer cells under the given conditions.investigation of cancer metabolism and to understand the role of microenvironment on cancerprogression.The long experimental protocols with 'Live cell setup' (aided with controlled CO2 delivery) demandedthe prevention of phototoxicity while collecting fluorescence data from the pH-sensitive dye cSNARFAM. By avoiding any scanning time, as required in confocal imaging, phototoxicity was majorly avoidedby recruiting the wide-field imaging technique. Moreover, since the dye was intracellularly activatedin viable cells the efficiency of the 'Wide-Field Microscopy' technique provided the dynamic control ofthe researcher over the experimental manoeuvre and while perfusing the cells with the experimentalsolutions and buffers. The unique arrangement and range of the filter sets and flexibility of reorganising their setup further helped the experiment process to use different models

SLIM_COMP_NH-1

Uploaded: Oct 04, 2017
Description: Nicola HumphryThis is a human osteosarcoma cell co-expressing an RFP-tagged mutant of survivin, and GFP-tagged microtubule-associated protein light chain 3 (LC3) to highlight autophagosomes, with NucBlue Live Ready Probe (Thermo Fisher Scientific) staining the nuclei. The dense RFP signal around the nuclear membrane was an unexpected but fascinating result, and represents how science can frustrate and surprise in equal measure. The image is a projection of a deconvolved z-stack of live cells taken with the DeltaVision Elite widefield microscope.

SLIM_COMP_NH-2

Uploaded: Oct 04, 2017
Description: Nicola HumphryThis is a bi-nucleated human osteosarcoma cell co-expressing an RFP-tagged histone H2B, and cerulean-tagged survivin. What can I say – it looks like an alien! The image is a projection of a deconvolved z-stack of live cells taken with the DeltaVision Elite widefield microscope.

SLIM_COMP_NH-3

Uploaded: Oct 04, 2017
Description: Nicola HumphryThis is a human osteosarcoma cell expressing GFP-tagged microtubule-associated protein light chain 3 (LC3) to highlight autophagosomes, with NucBlue Live Ready Probe (Thermo Fisher Scientific) staining the nuclei. The cell has been treated with a drug to accumulate autophagosomes. This is the clearest image I have taken of autophagosomes, showing the LC3-decorated membranes of spherical bodies of varying size in the cytoplasm. The image is a projection of a deconvolved z-stack of live cells taken with the DeltaVision Elite widefield microscope.

STAT2 version2crop1 (2)

Uploaded: Oct 04, 2017
Description: Uwe VinkemeierWhat is it? Fluorescent micrograph of HeLa cells transfected with STAT2 (green) and stained for nuclei (blue) and STAT1 (red). Nuclei with STAT1 appear intensely pink, in contrast to the dark blue holes seen in STAT2-expressing cells, which demonstrates the inhibition of STAT1 nuclear import and function by STAT2.What does it mean? STAT2 is known for more than a quarter century to bind to STAT1. This interaction was thought to be required solely to promote type 1 interferon responses. The image shows that the long-known interaction of STAT2 and STAT1 has an important additional function, namely to inhibit cytokine activities. The discovery of STAT2's duplicitous nature was published in the October 2016 issue of PLOS Biology (Ho et al.), when this image was used as the backdrop for the journal's homepage.

SW Image 1

Uploaded: Oct 04, 2017
Description: Sally WheatleyWheatley - Image 1.Human cervical cancer cells (HeLa) ectopically expressing survivin-GFP (green), imaged live on the wide field Delta Vision inverted fluorescence microscope using x63 (NA 1.4) lens. A single 0.3 micron optical slice is shown and cells are labelled with mitotracker to visualise the mitochondria (red), and nucleoblue to visualise the nuclei (blue). Cells were imaged 5 minutes after addition of fluorescent dyes, and have not fully integrated into their respective organelles, thus giving a gradient effect to the labelling (red and blue). Cells are approximately 70 microns in size.

SW Image 2

Uploaded: Oct 04, 2017
Description: Sally WheatleyWheatley - Image 2.Human cervical cancer cell (HeLa) ectopically expressing survivin-GFP (green) undergoing aberrant mitosis. The cell is in metaphase and preparing to divide into three. Genomic instability of this sort is a hallmark of cancer. At this stage survivin is found at the centromeres, the button-like constrictions at the centre of each chromosome. The cell was fixed with formaldehyde, permeablised with triton-x-100, and immunostained with anti-tubulin antibodies to reveal the microtubules (red), before counterstaining with DAPI to visualise the chromosomes (blue). The cell was imaged in 0.3 micron optical slices using the Delta Vision inverted fluorescence microscope using x63 (NA 1.4) lens, which were then deconvolved. A 2D projection of the 3D stack is presented and was prepared using Adobe Photoshop. Cell diameter approximately 100 microns.

SW image 3

Uploaded: Oct 04, 2017
Description: Sally WheatleyWheatley - Image 3. Human cervical cancer cells fixed and immunostained to reveal the focal adhesions with anti-vinculin antibodies (green), and counterstained with rhodamine phalloidin to visualise actin filaments (red), and DAPI to highlight the nucleus (blue). The cell was imaged in 0.3 micron optical slices using the Delta Vision inverted fluorescence microscope using x63 (NA 1.4) lens, which were then deconvolved. A 2D projection of the 3D stack is presented and was prepared using Adobe Photoshop.

WT T cells No TCRed but PHAed_02-Z-stack CH-1

Uploaded: Oct 04, 2017
Description: Cheng HuaitaoThe first and second one (WT, T cells No TCRed and PHDH2-ALL10..) are respectively WT DEF6 and DEF6 PHDH2-All-10 mutant expressed in resting T cells (Jurkat T cell line). They both localised indicated cell protrusions. These cells were also stained by red phalloidine that binds to F-actin.

July 2017 Image Competition

0aa_W3CMSNP2a__Goulding_print_cropWeb

0bRuiter_3T3 cells_Printweb

0cSLIM Image Competition - Othman_Print

533P-356P YES TCRed Pha~ 04 CH-3

6mA vs 5mC in LN18 mitotic cells with DAPI as counterstain

image 1 YA

image 2 YA

image 3 YA

Microalgae_with scale

Neurons - NT

PHDH2-ALL10 No TCRed ~03 CH-2

SLIM competition 2017

SLIM IMAGE_U.B._4181919

SLIM_COMP_NH-1

SLIM_COMP_NH-2

SLIM_COMP_NH-3

STAT2 version2crop1 (2)

SW Image 1

SW Image 2

SW image 3

WT T cells No TCRed but PHAed_02-Z-stack CH-1

School of Life Sciences

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