Fluorescence and Confocal Microscopy
Equipment
- Zeiss LSM880 confocal laser scanning microscope. The Zeiss LSM 880 is a modern, flexible, confocal fluorescence microscope capable of looking at both fixed and live cells / animals.
- DeltaVision Elite widefield restoration/deconvolution microscope. The DeltaVision Elite imaging system is a deconvolution fluorescence microscope system optimized for low light and live cell imaging applications.
Equipped with temperature controlled environmental chamber, CO2 and humidity for live-cell imaging. Laser module (405, 488, 514, 565nm) for FRAP, FLIP and TIRF imaging.
- Leica SP2 confocal laser scanning microscope (CLSM). An inverted system with heated stage, environmental chamber and laser lines from 405nm (UV) to 633nm (far red). Equipped with a spectrophotometer scan head allowing user-designed fluorescence detection across the whole spectrum. Laser lines available are 405nm, 448nm, 476nm, 488nm, 514nm, 543nm and 633nm. This system is particularly useful for live cell imaging using GFP, CFP and YFP, time lapse imaging and 3D reconstruction.
- Leica IRE2 time-lapse DiC and fluorescence microscope with full stage incubation (37C, 5% CO2) and a Hammamatsu OrcaER monochrome camera. For longer time-lapse and multi-channel acquisitions (filters for FITC/GFP, TRITC, DAPI) and image analysis.
- Zeiss Axiophot upright fluorescence microscope with filters for DAPI, FITC and Texas red. Equipped with a monochrome digital camera (Hammamatsu C4742-95) and Openlab acquisition and analysis software.
Applications
- Confocal imaging of live/fixed cells, tissues and cell-material tissue engineering constructs. 3D and 4D image reconstruction.
- Region of interest (ROI) scanning, FRET, FRAP, TIRF and image quantification.
- Live-cell, multi-channel time-lapse imaging of cultured cells or fluorescence/GFP-tagged novel molecules.
- Fluorescence imaging of tissues, cells and clinical samples in multiple channels.
- Reflectance imaging of surfaces and materials to visualise material surfaces, polymer scaffolds or to provide roughness measurements.
Image Analysis
Image analysis is supported within SLIM by a dedicated team member, Seema Rajana, new projects should be initiated through Tim Self. More information about our image analysis facilities and experience can be found on the associated pages.
Contacts
For advice, discussions regarding experimental design for light or fluorescence microscopy please contact Tim Self.
For advice with grant applications, or general access queries, please contact Tim Self.
Selected Recent Publications
Jennifer T. Patel, Hannah R. Belsham, Alexandra J. Rathbone, Bill Wickstead, Christopher Gell, Claire T. Friel The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition Open Biol. 2016 6 160223; DOI: 10.1098/rsob.160223.
Dunajová L, Cash E, Markus R, Rochette S, Townley AR, Wheatley SP. The NH2terminus of survivin is a mitochondrial targeting sequence and C-Src regulator. JCell Sci. 2016 May 31. pii: jcs.183277.
Xu L, Nwosu LN, Burston JJ, Millns PJ, Sagar DR, Mapp PI, Meesawatsom P, Li L,Bennett AJ, Walsh DA, Chapman V. The anti-NGF antibody muMab 911 both preventsand reverses pain behaviour and subchondral osteoclast numbers in a rat model of osteoarthritis pain. Osteoarthritis Cartilage. 2016 May 18. pii:S1063-4584(16)30101-7. doi: 10.1016/j.joca.2016.05.015
Goode A, Butler K, Long J, Cavey J, Scott D, Shaw B, Sollenberger J, Gell C, Johansen T, Oldham NJ, Searle MS, Layfield R. Defective recognition of LC3B bymutant SQSTM1/p62 implicates impairment of autophagy as a pathogenic mechanism inALS-FTLD. Autophagy. 2016 May 9:1-11.
Wheatley SP. The functional repertoire of survivin's tails. Cell Cycle. 2015;14(2):261-8.
Aljaberi AM, Webster JR, Wheatley SP. Analysis of the functional repertoire of a mutant form of survivin, K129E, which has been linked to lung cancer. Cancer Cell Int. 2014 Aug 19;14:78.
Burston JJ, Sagar DR, Shao P, Bai M, King E, Brailsford L, Turner JM, Hathway GJ, Bennett AJ, Walsh DA, Kendall DA, Lichtman A, Chapman V. Cannabinoid CB2 receptors regulate central sensitization and pain responses associated with osteoarthritis of the knee joint. PLoS One. 2013 Nov 25;8(11):e80440.
View all SLIM publications